Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus

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cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.contributor.donorSwedish International Development Cooperation Agencyen_US
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen_US
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen_US
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen_US
cg.contributor.donorGoogleen_US
cg.coverage.countryUgandaen_US
cg.coverage.iso3166-alpha2UGen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970en_US
cg.creator.identifierAlan S. Orth: 0000-0002-1735-7458en_US
cg.creator.identifierAppolinaire Djikeng: 0000-0001-9271-3419en_US
cg.creator.identifierStephen J Kemp: 0000-0003-4041-1720en_US
cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1186/1743-422x-9-218en_US
cg.isijournalISI Journalen_US
cg.issn1743-422Xen_US
cg.issue1en_US
cg.journalVirology Journalen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriAGRI-HEALTHen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriANIMAL HEALTHen_US
cg.subject.ilriEMERGING DISEASESen_US
cg.subject.ilriHEALTHen_US
cg.subject.ilriPIGSen_US
cg.subject.ilriZOONOTIC DISEASESen_US
cg.volume9en_US
dc.contributor.authorMasembe, C.en_US
dc.contributor.authorMichuki, George N.en_US
dc.contributor.authorOnyango, M.en_US
dc.contributor.authorRumberia, C.en_US
dc.contributor.authorNorling M.en_US
dc.contributor.authorBishop, Richard P.en_US
dc.contributor.authorDjikeng, Appolinaireen_US
dc.contributor.authorKemp, Stephen J.en_US
dc.contributor.authorOrth, Alan S.en_US
dc.contributor.authorSkilton, Robert A.en_US
dc.contributor.authorStahl, K.en_US
dc.contributor.authorFischer, A.en_US
dc.date.accessioned2013-03-10T10:33:26Zen_US
dc.date.available2013-03-10T10:33:26Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/27684en_US
dc.titleViral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virusen_US
dcterms.abstractThe rising demand for pork has resulted in a massive expansion of pig production in Uganda. This has resulted in increased contact between humans and pigs. Pigs can act as reservoirs for emerging infectious diseases. Therefore identification of potential zoonotic pathogens is important for public health surveillance. In this study, during a routine general surveillance for African swine fever, domestic pigs from Uganda were screened for the presence of RNA and DNA viruses using a high-throughput pyrosequencing method. Serum samples from 16 domestic pigs were collected from five regions in Uganda and pooled accordingly. Genomic DNA and RNA were extracted and sequenced on the 454 GS-FLX platform. Among the sequences assigned to a taxon, 53% mapped to the domestic pig (Sus scrofa). African swine fever virus, Torque teno viruses (TTVs), and porcine endogenous retroviruses were identified. Interestingly, two pools (B and C) of RNA origin had sequences that showed 98% sequence identity to Ndumu virus (NDUV). None of the reads had identity to the class Insecta indicating that these sequences were unlikely to result from contamination with mosquito nucleic acids. This is the first report of the domestic pig as a vertebrate host for Ndumu virus. NDUV had been previously isolated only from culicine mosquitoes. NDUV therefore represents a potential zoonotic pathogen, particularly given the increasing risk of human-livestock-mosquito contact.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2012-09-24en_US
dcterms.bibliographicCitationMasembe, C., Michuki, G., Onyango, M., Rumberia, C., Norling M., Bishop, R.P., Djikeng, A., Kemp, S.J., Orth, A., Skilton, R.A., Ståhl, K. and Fischer, A. 2012. Viral metagenomics demonstrates that domestic pigs are a potential reservoir for Ndumu virus. Virology Journal 9:218en_US
dcterms.issued2012-12en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-2.0en_US
dcterms.publisherSpringeren_US
dcterms.subjectanimal diseasesen_US
dcterms.subjectswineen_US
dcterms.subjectzoonosesen_US
dcterms.subjectinfectious diseasesen_US
dcterms.subjectvirologyen_US
dcterms.subjecthealthen_US
dcterms.typeJournal Articleen_US

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