The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationSwedish University of Agricultural Sciencesen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationFunctional Genomics Centre, Switzerlanden
cg.contributor.affiliationUniversity of Marylanden
cg.contributor.affiliationUniversity of Nairobien
cg.contributor.affiliationSwiss Tropical and Public Health Instituteen
cg.contributor.affiliationUniversity of Baselen
cg.contributor.crpLivestock and Fish
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierSonal P Henson: 0000-0002-2002-5462
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970
cg.creator.identifierRoger Pelle: 0000-0003-1053-085X
cg.creator.identifierErik Bongcam-Rudloff: 0000-0002-1947-8288
cg.creator.identifierDavid Odongo: 0000-0001-5745-4327
cg.creator.identifierClaudia Daubenberger: 0000-0001-7136-0642
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1186/s12864-015-1910-9en
cg.isijournalISI Journalen
cg.issn1471-2164en
cg.issue1en
cg.journalBMC Genomicsen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriCATTLEen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriECFen
cg.subject.ilriVACCINESen
cg.volume16en
dc.contributor.authorNorling, M.en
dc.contributor.authorBishop, Richard P.en
dc.contributor.authorPelle, Rogeren
dc.contributor.authorWeihong Qien
dc.contributor.authorHenson, S.en
dc.contributor.authorDrábek, E.F.en
dc.contributor.authorTretina, Kyleen
dc.contributor.authorOdongo, D.en
dc.contributor.authorMwaura, S.en
dc.contributor.authorNjoroge, T.en
dc.contributor.authorBongcam-Rudloff, E.en
dc.contributor.authorDaubenberger, C.A.en
dc.contributor.authorSilva, Joana C.en
dc.date.accessioned2016-01-05T17:41:12Zen
dc.date.available2016-01-05T17:41:12Zen
dc.identifier.urihttps://hdl.handle.net/10568/69467
dc.titleThe genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergenceen
dcterms.abstractThere are no commercially available vaccines against human protozoan parasitic diseases, despite the success of vaccination-induced long-term protection against infectious diseases. East Coast fever, caused by the protist Theileria parva, kills one million cattle each year in sub-Saharan Africa, and contributes significantly to hunger and poverty in the region. A highly effective, live, multi-isolate vaccine against T. parva exists, but its component isolates have not been characterized. Here we sequence and compare the three component T. parva stocks within this vaccine, the Muguga Cocktail, namely Muguga, Kiambu5 and Serengeti-transformed, aiming to identify genomic features that contribute to vaccine efficacy. We find that Serengeti-transformed, originally isolated from the wildlife carrier, the African Cape buffalo, is remarkably and unexpectedly similar to the Muguga isolate. The 420 detectable non-synonymous SNPs were distributed among only 53 genes, primarily subtelomeric antigens and antigenic families. The Kiambu5 isolate is considerably more divergent, with close to 40,000 SNPs relative to Muguga, including >8,500 non-synonymous mutations distributed among >1,700 (42.5 %) of the predicted genes. These genetic markers of the component stocks can be used to characterize the composition of new batches of the Muguga Cocktail. Differences among these three isolates, while extensive, represent only a small proportion of the genetic variation in the entire species. Given the efficacy of the Muguga Cocktail in inducing long-lasting protection against infections in the field, our results suggest that whole-organism vaccines against parasitic diseases can be highly efficacious despite considerable genome-wide differences relative to the isolates against which they protect.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2015-09-24
dcterms.bibliographicCitationNorling, M., Bishop, R.P., Pelle, R., Weihong Qi, Henson, S., Drábek, E.F., Tretina, K., Odongo, D., Mwaura, S., Njoroge, T., Bongcam-Rudloff, E., Daubenberger, C.A. and Silva, J.C. 2016. The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence. BMC Genomics 16:729.en
dcterms.issued2015-12
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectanimal diseasesen
dcterms.subjectgeneticsen
dcterms.subjectbiotechnologyen
dcterms.typeJournal Article

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