The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence

Simple item page
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationSwedish University of Agricultural Sciencesen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationFunctional Genomics Centre, Switzerlanden_US
cg.contributor.affiliationUniversity of Marylanden_US
cg.contributor.affiliationUniversity of Nairobien_US
cg.contributor.affiliationSwiss Tropical and Public Health Instituteen_US
cg.contributor.affiliationUniversity of Baselen_US
cg.contributor.crpLivestock and Fishen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierSonal P Henson: 0000-0002-2002-5462en_US
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970en_US
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen_US
cg.creator.identifierErik Bongcam-Rudloff: 0000-0002-1947-8288en_US
cg.creator.identifierDavid Odongo: 0000-0001-5745-4327en_US
cg.creator.identifierClaudia Daubenberger: 0000-0001-7136-0642en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1186/s12864-015-1910-9en_US
cg.isijournalISI Journalen_US
cg.issn1471-2164en_US
cg.issue1en_US
cg.journalBMC Genomicsen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriECFen_US
cg.subject.ilriVACCINESen_US
cg.volume16en_US
dc.contributor.authorNorling, M.en_US
dc.contributor.authorBishop, Richard P.en_US
dc.contributor.authorPelle, Rogeren_US
dc.contributor.authorWeihong Qien_US
dc.contributor.authorHenson, S.en_US
dc.contributor.authorDrábek, E.F.en_US
dc.contributor.authorTretina, Kyleen_US
dc.contributor.authorOdongo, D.en_US
dc.contributor.authorMwaura, S.en_US
dc.contributor.authorNjoroge, T.en_US
dc.contributor.authorBongcam-Rudloff, E.en_US
dc.contributor.authorDaubenberger, C.A.en_US
dc.contributor.authorSilva, Joana C.en_US
dc.date.accessioned2016-01-05T17:41:12Zen_US
dc.date.available2016-01-05T17:41:12Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/69467en_US
dc.titleThe genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergenceen_US
dcterms.abstractThere are no commercially available vaccines against human protozoan parasitic diseases, despite the success of vaccination-induced long-term protection against infectious diseases. East Coast fever, caused by the protist Theileria parva, kills one million cattle each year in sub-Saharan Africa, and contributes significantly to hunger and poverty in the region. A highly effective, live, multi-isolate vaccine against T. parva exists, but its component isolates have not been characterized. Here we sequence and compare the three component T. parva stocks within this vaccine, the Muguga Cocktail, namely Muguga, Kiambu5 and Serengeti-transformed, aiming to identify genomic features that contribute to vaccine efficacy. We find that Serengeti-transformed, originally isolated from the wildlife carrier, the African Cape buffalo, is remarkably and unexpectedly similar to the Muguga isolate. The 420 detectable non-synonymous SNPs were distributed among only 53 genes, primarily subtelomeric antigens and antigenic families. The Kiambu5 isolate is considerably more divergent, with close to 40,000 SNPs relative to Muguga, including >8,500 non-synonymous mutations distributed among >1,700 (42.5 %) of the predicted genes. These genetic markers of the component stocks can be used to characterize the composition of new batches of the Muguga Cocktail. Differences among these three isolates, while extensive, represent only a small proportion of the genetic variation in the entire species. Given the efficacy of the Muguga Cocktail in inducing long-lasting protection against infections in the field, our results suggest that whole-organism vaccines against parasitic diseases can be highly efficacious despite considerable genome-wide differences relative to the isolates against which they protect.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2015-09-24en_US
dcterms.bibliographicCitationNorling, M., Bishop, R.P., Pelle, R., Weihong Qi, Henson, S., Drábek, E.F., Tretina, K., Odongo, D., Mwaura, S., Njoroge, T., Bongcam-Rudloff, E., Daubenberger, C.A. and Silva, J.C. 2016. The genomes of three stocks comprising the most widely utilized live sporozoite Theileria parva vaccine exhibit very different degrees and patterns of sequence divergence. BMC Genomics 16:729.en_US
dcterms.issued2015-12en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherSpringeren_US
dcterms.subjectanimal diseasesen_US
dcterms.subjectgeneticsen_US
dcterms.subjectbiotechnologyen_US
dcterms.typeJournal Articleen_US

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
ILRI Tick unit
ILRI vaccine biosciences program articles
Livestock Fish journal articles