Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections

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cg.contributor.affiliationInstitute of Tropical Medicine, Antwerpen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationKatholieke Universiteit Leuvenen
cg.contributor.donorResearch Foundation Flandersen
cg.identifier.doihttps://doi.org/10.1186/1475-9292-3-3en
cg.issn1475-9292en
cg.issue1en
cg.journalKinetoplastid Biology and Diseaseen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriTRYPANOSOMIASISen
cg.volume3en
dc.contributor.authorClaes, Filipen
dc.contributor.authorRadwanska, Magdaen
dc.contributor.authorUrakawa, Toyoen
dc.contributor.authorMajiwa, Phelix A.O.en
dc.contributor.authorGoddeeris, Bruno M.en
dc.contributor.authorBüscher, Philippeen
dc.date.accessioned2023-03-10T14:33:02Zen
dc.date.available2023-03-10T14:33:02Zen
dc.identifier.urihttps://hdl.handle.net/10568/129298
dc.titleVariable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infectionsen
dcterms.abstractBased on the recently sequenced gene coding for the Trypanosoma evansi (T. evansi) RoTat 1.2 Variable Surface Glycoprotein (VSG), a primer pair was designed targeting the DNA region lacking homology to other known VSG genes. A total of 39 different trypanosome stocks were tested using the RoTat 1.2 based Polymerase Chain Reaction (PCR).This PCR yielded a 205 bp product in all T. evansi and in seven out of nine T. equiperdum strains tested. This product was not detected in the DNA from T. b. brucei, T. b. gambiense, T. b. rhodesiense, T. congolense, T. vivax and T. theileri parasites. The Rotat 1.2 PCR detects as few as 10 trypanosomes per reaction with purified DNA from blood samples, i.e. 50 trypanosomes/ml.PCR amplification of the RoTat 1.2 VSG gene is a specific marker for T. evansi strains, except T. evansi type B, and is especially useful in dyskinetoplastic strains where kDNA based markers may fail to amplify. Furthermore, our data support previous suggestions that some T. evansi stocks have been previously misclassified as T. equiperdum.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationClaes, Filip; Radwanska, Magda; Urakawa, Toyo; Majiwa, Phelix A.O.; Goddeeris, Bruno M.; Büscher, Philippe. 2004. Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections. Kinetoplastid Biology and Disease 3: 3en
dcterms.extent3en
dcterms.issued2004
dcterms.languageen
dcterms.licenseOther
dcterms.publisherSpringeren
dcterms.subjectpcren
dcterms.subjecttrypanosoma evansien
dcterms.subjectdetectionen
dcterms.subjectglycoproteinen
dcterms.subjectdiagnosticen
dcterms.typeJournal Article

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