Ensuring the phytosanitary quality of beans, tropical forages and cassava collections in the “future seeds” genebank: reference strain bank

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cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Center for Tropical Agricultureen_US
cg.contributor.affiliationUniversidad ICESIen_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.initiativeGenebanksen_US
cg.coverage.countryColombiaen_US
cg.coverage.iso3166-alpha2COen_US
cg.coverage.regionAmericasen_US
cg.coverage.regionSouth Americaen_US
cg.coverage.regionLatin America and the Caribbeanen_US
cg.creator.identifierJulio César Ramírez Pretelt: 0000-0003-3364-9562en_US
cg.creator.identifierDiana Patricia Niño Jimenez: 0000-0002-3387-3401en_US
cg.creator.identifierMaritza Cuervo Ibañez: 0000-0002-4418-699Xen_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.alliancebiovciatBEANSen_US
cg.subject.alliancebiovciatHEALTHen_US
cg.subject.alliancebiovciatPLANT GENETIC RESOURCESen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
dc.contributor.authorRamirez Pretelt, Julio Cesaren_US
dc.contributor.authorNiño Jimenez, Diana Patriciaen_US
dc.contributor.authorMuñoz Molina, Christian Daviden_US
dc.contributor.authorDorado Escobar, Edwin Fabianen_US
dc.contributor.authorCuervo Ibanez, Maritzaen_US
dc.contributor.authorVélez-Rojas, Katalinaen_US
dc.date.accessioned2024-11-07T13:17:52Zen_US
dc.date.available2024-11-07T13:17:52Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/159387en_US
dc.titleEnsuring the phytosanitary quality of beans, tropical forages and cassava collections in the “future seeds” genebank: reference strain banken_US
dcterms.abstractThe Germplasm Health Unit of the Bioversity International Alliance and CIAT (GHU-CIAT) ensures that the germplasm of beans, cassava and tropical forages conserved and distributed nationally and internationally by the Germplasm Genebank, and breeding programs are free of quarantine risk pests and diseases. As part of routine activities, GHU-CIAT detects the presence of phytopathogenic fungi using morphological and molecular techniques. In order to ensure the quality and traceability of the results, the GHU-CIAT has created a reference strains bank with those quarantine fungi that can historically be detected in the germplasm evaluated under laboratory conditions. Initially, fungal isolates were identified and characterized morphologically by using conventional taxonomic keys, including photographs of macro and micro morphological features. Additionally, a protocol for DNA extraction was standardized and using Oxford Nanopore Technology, isolates were sequenced and molecularly characterized. Using this methodology, 40 isolates were obtained corresponding to the following genera: Colletotrichum sp., Phoma sp., Alternaria, Curvularia sp., Bipolaris sp., Pestalotiopsis sp., and Fusarium sp. Finally, the morphologically and molecularly characterized strains were conserved under controlled temperature and luminosity conditions. Having this kind of reference strain ensures the quality of the results obtained in the diagnosis of phytopathogenic fungi and works as a quality control mechanism which to evidence and demonstrate the accuracy in the identification and detection of quarantine fungi. This study strengthens the traceability for the validation of the diagnostic method and additionally provides information about the genetic variability of the different species detected. Keywords: Reference strain, Quarantine, Sequencing, Traceability.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.bibliographicCitationRamirez Pretelt, J.C.; Niño Jimenez, D.P.; Muñoz Molina, C.D.; Dorado Escobar, E.F.; Cuervo Ibanez, M.; Vélez-Rojas, K. (2024) Ensuring the phytosanitary quality of beans, tropical forages and cassava collections in the “future seeds” genebank: reference strain bank. Poster presented at the “XX International Plant Protection Congress” on1-5 July 2024 in Athens (Greece). 1 p.en_US
dcterms.extent1 p.en_US
dcterms.issued2024-07-05en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.subjectbeansen_US
dcterms.subjectplant genetic resourcesen_US
dcterms.subjectgene banksen_US
dcterms.subjecttraceabilityen_US
dcterms.subjectquarantineen_US
dcterms.typePosteren_US

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ENSURING THE PHYTOSANITARY QUALITY OF BEANS, TROPICAL FORAGES AND CASSAVA COLLECTIONS IN THE “FUTURE SEEDS” GENEBANK - REFERENCE STRAIN BANK.pdf
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