Species-informative SNP markers for characterising freshwater prawns of genus Macrobrachium in Cameroon

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationUniversity of Buéaen
cg.contributor.affiliationUSOMI Ltden
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationCommonwealth Scientific and Industrial Research Organisation, Australiaen
cg.contributor.affiliationUniversity of Doualaen
cg.contributor.affiliationConcepto Azul, Ecuadoren
cg.contributor.affiliationInstitut de Recherche Agricole pour le Développement, Cameroonen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.coverage.countryCameroonen
cg.coverage.iso3166-alpha2CMen
cg.coverage.regionAfricaen
cg.coverage.regionWestern Africaen
cg.coverage.regionMiddle Africaen
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1371/journal.pone.0263540en
cg.isijournalISI Journalen
cg.issn1932-6203en
cg.issue10en
cg.journalPLOS ONEen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL BREEDINGen
cg.subject.ilriGENETICSen
cg.volume17en
dc.contributor.authorMakombu, J.G.en
dc.contributor.authorCheruiyot, E.K.en
dc.contributor.authorStomeo, Francescaen
dc.contributor.authorThuo, D.N.en
dc.contributor.authorOben, P.M.en
dc.contributor.authorOben, Benedicta O.en
dc.contributor.authorZango, P.en
dc.contributor.authorMialhe, E.en
dc.contributor.authorNgueguim, J.R.en
dc.contributor.authorMujibi, F.D.N.en
dc.date.accessioned2022-10-19T12:55:51Zen
dc.date.available2022-10-19T12:55:51Zen
dc.identifier.urihttps://hdl.handle.net/10568/125118
dc.titleSpecies-informative SNP markers for characterising freshwater prawns of genus Macrobrachium in Cameroonen
dcterms.abstractSingle Nucleotide Polymorphisms (SNPs) are now popular for a myriad of applications in animal and plant species including, ancestry assignment, conservation genetics, breeding, and traceability of animal products. The objective of this study was to develop a customized cost-effective SNP panel for genetic characterisation of Macrobrachium species in Cameroon. The SNPs identified in a previous characterization study were screened as viable candidates for the reduced panel. Starting from a full set of 1,814 SNPs, a total of 72 core SNPs were chosen using conventional approaches: allele frequency differentials, minor allele frequency profiles, and Wright’s Fst statistics. The discriminatory power of reduced set of informative SNPs were then tested using the admixture analysis, principal component analysis, and discriminant analysis of principal components. The panel of prioritised SNP markers (i.e., N = 72 SNPs) distinguished Macrobrachium species with 100% accuracy. However, large sample size is needed to identify more informative SNPs for discriminating genetically closely related species, including M. macrobrachion versus M. vollenhovenii and M. sollaudii versus M. dux. Overall, the findings in this study show that we can accurately characterise Macrobrachium using a small set of core SNPs which could be useful for this economically important species in Cameroon. Given the results obtained in this study, a larger independent validation sample set will be needed to confirm the discriminative capacity of this SNP panel for wider commercial and research applications.en
dcterms.accessRightsOpen Accessen
dcterms.audienceScientistsen
dcterms.available2022-10-03en
dcterms.bibliographicCitationMakombu, J.G., Cheruiyot, E.K., Stomeo, F., Thuo, D.N., Oben, P.M., Oben, B.O., Zango, P., Mialhe, E., Ngueguim, J.R., Mujibi, F.D.N. 2022. Species-informative SNP markers for characterising freshwater prawns of genus Macrobrachium in Cameroon. PLoS ONE 17(10):e0263540.en
dcterms.issued2022-10-03en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherPublic Library of Scienceen
dcterms.subjectmacrobrachiumen
dcterms.subjectsingle nucleotide polymorphismen
dcterms.subjectgenetic markersen
dcterms.subjectfreshwater prawns and shrimpsen
dcterms.subjectgeneticsen
dcterms.subjectanimal breedingen
dcterms.typeJournal Articleen

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