Microbial Community Structure and Diversity in an Integrated System of Anaerobic-Aerobic Reactors and a Constructed Wetland for the Treatment of Tannery Wastewater in Modjo, Ethiopia

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cg.contributor.affiliationAddis Ababa Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.coverage.countryEthiopia
cg.coverage.iso3166-alpha2ET
cg.coverage.regionEastern Africa
cg.creator.identifierAppolinaire Djikeng: 0000-0001-9271-3419
cg.identifier.doihttps://doi.org/10.1371/journal.pone.0115576en
cg.issn1932-6203en
cg.issue12en
cg.journalPLOS ONEen
cg.subject.ilriWASTEen
cg.volume9en
dc.contributor.authorDesta, Adey Felekeen
dc.contributor.authorAssefa, Fassilen
dc.contributor.authorLeta, Seyoumen
dc.contributor.authorStomeo, Francescaen
dc.contributor.authorWamalwa, Marken
dc.contributor.authorNjahira, Moses N.en
dc.contributor.authorDjikeng, Appolinaireen
dc.date.accessioned2023-03-10T14:40:12Zen
dc.date.available2023-03-10T14:40:12Zen
dc.identifier.urihttps://hdl.handle.net/10568/129582
dc.titleMicrobial Community Structure and Diversity in an Integrated System of Anaerobic-Aerobic Reactors and a Constructed Wetland for the Treatment of Tannery Wastewater in Modjo, Ethiopiaen
dcterms.abstractA culture-independent approach was used to elucidate the microbial diversity and structure in the anaerobic-aerobic reactors integrated with a constructed wetland for the treatment of tannery wastewater in Modjo town, Ethiopia. The system has been running with removal efficiencies ranging from 94%–96% for COD, 91%–100% for SO42- and S2-, 92%–94% for BOD, 56%–82% for total Nitrogen and 2%–90% for NH3-N. 16S rRNA gene clone libraries were constructed and microbial community assemblies were determined by analysis of a total of 801 unique clone sequences from all the sites. Operational Taxonomic Unit (OTU) - based analysis of the sequences revealed highly diverse communities in each of the reactors and the constructed wetland. A total of 32 phylotypes were identified with the dominant members affiliated to Clostridia (33%), Betaproteobacteria (10%), Bacteroidia (10%), Deltaproteobacteria (9%) and Gammaproteobacteria (6%). Sequences affiliated to the class Clostridia were the most abundant across all sites. The 801 sequences were assigned to 255 OTUs, of which 3 OTUs were shared among the clone libraries from all sites. The shared OTUs comprised 80 sequences belonging to Clostridiales Family XIII Incertae Sedis, Bacteroidetes and unclassified bacterial group. Significantly different communities were harbored by the anaerobic, aerobic and rhizosphere sites of the constructed wetland. Numerous representative genera of the dominant bacterial classes obtained from the different sample sites of the integrated system have been implicated in the removal of various carbon- containing pollutants of natural and synthetic origins. To our knowledge, this is the first report of microbial community structure in tannery wastewater treatment plant from Ethiopia.en
dcterms.accessRightsOpen Access
dcterms.available2014-12-26
dcterms.bibliographicCitationDesta, Adey Feleke; Assefa, Fassil; Leta, Seyoum; Stomeo, Francesca; Wamalwa, Mark; Njahira, Moses N.; Djikeng, Appolinaire. 2014. Microbial Community Structure and Diversity in an Integrated System of Anaerobic-Aerobic Reactors and a Constructed Wetland for the Treatment of Tannery Wastewater in Modjo, Ethiopia. PLoS ONE 9: e115576en
dcterms.extente115576en
dcterms.issued2014-12-26
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherPublic Library of Scienceen
dcterms.subjectwastewateren
dcterms.subjectethiopiaen
dcterms.subjectstructureen
dcterms.subjectsystemen
dcterms.subjectwetlandsen
dcterms.typeJournal Article

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