Broad-spectrum resistance to bacterial blight in rice using genome editing

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cg.identifier.doihttps://doi.org/10.1038/s41587-019-0267-zen
cg.issn1087-0156en
cg.issue11en
cg.journalNature Biotechnologyen
cg.volume37en
dc.contributor.authorOliva, Ricardoen
dc.contributor.authorJi, Chonghuien
dc.contributor.authorAtienza-Grande, Genelouen
dc.contributor.authorHuguet-Tapia, José C.en
dc.contributor.authorPerez-Quintero, Alvaroen
dc.contributor.authorLi, Tingen
dc.contributor.authorEom, Joon-Seoben
dc.contributor.authorLi, Chenhaoen
dc.contributor.authorNguyen, Hannaen
dc.contributor.authorLiu, Boen
dc.contributor.authorAuguy, Florenceen
dc.contributor.authorSciallano, Colineen
dc.contributor.authorLuu, Van T.en
dc.contributor.authorDossa, Gerbert S.en
dc.contributor.authorCunnac, Sébastienen
dc.contributor.authorSchmidt, Sarah M.en
dc.contributor.authorSlamet-Loedin, Inez H.en
dc.contributor.authorVera Cruz, Casianaen
dc.contributor.authorSzurek, Borisen
dc.contributor.authorFrommer, Wolf B.en
dc.contributor.authorWhite, Frank F.en
dc.contributor.authorYang, Bingen
dc.date.accessioned2024-12-19T12:54:06Zen
dc.date.available2024-12-19T12:54:06Zen
dc.identifier.urihttps://hdl.handle.net/10568/164605
dc.titleBroad-spectrum resistance to bacterial blight in rice using genome editingen
dcterms.abstractBacterial blight of rice is an important disease in Asia and Africa. The pathogen,Xanthomonas oryzaepv.oryzae(Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genesSWEET11,SWEET13andSWEET14, the expression of which is required for disease susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations in all threeSWEETgene promoters. Editing was further informed by sequence analyses of TALe genes in 63Xoostrains, which revealed multiple TALe variants forSWEET13alleles. Mutations were also created inSWEET14, which is also targeted by two TALes from an AfricanXoolineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-editedSWEETpromoters endow rice lines with robust, broad-spectrum resistance.en
dcterms.accessRightsOpen Access
dcterms.available2019-10-28
dcterms.bibliographicCitationOliva, Ricardo; Ji, Chonghui; Atienza-Grande, Genelou; Huguet-Tapia, José C.; Perez-Quintero, Alvaro; Li, Ting; Eom, Joon-Seob; Li, Chenhao; Nguyen, Hanna; Liu, Bo; Auguy, Florence; Sciallano, Coline; Luu, Van T.; Dossa, Gerbert S.; Cunnac, Sébastien; Schmidt, Sarah M.; Slamet-Loedin, Inez H.; Vera Cruz, Casiana; Szurek, Boris; Frommer, Wolf B.; White, Frank F. and Yang, Bing. 2019. Broad-spectrum resistance to bacterial blight in rice using genome editing. Nat Biotechnol, Volume 37 no. 11 p. 1344-1350en
dcterms.extentpp. 1344-1350en
dcterms.issued2019-11
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectapplied microbiology and biotechnologyen
dcterms.subjectbioengineeringen
dcterms.subjectbiomedical engineeringen
dcterms.subjectbiotechnologyen
dcterms.subjectmolecular medicineen
dcterms.typeJournal Article

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