Abundance and diversity of tomato rhizosphere microbes and their effect on bacterial wilt disease

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationMakerere Universityen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.donorAustrian Development Agencyen
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.placeKampala, Ugandaen
cg.subject.iitaAGRIBUSINESSen
cg.subject.iitaBIODIVERSITYen
cg.subject.iitaDISEASE CONTROLen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaFORESTRYen
cg.subject.iitaLAND USEen
cg.subject.iitaPESTS OF PLANTSen
cg.subject.iitaPLANT DISEASESen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.iitaRESEARCH METHODen
dc.contributor.authorNampamya, D.en
dc.date.accessioned2022-08-25T14:05:30Zen
dc.date.available2022-08-25T14:05:30Zen
dc.identifier.urihttps://hdl.handle.net/10568/120942
dc.titleAbundance and diversity of tomato rhizosphere microbes and their effect on bacterial wilt diseaseen
dcterms.abstractThe use of land for agricultural production is always accompanied with changes in microbial diversity and emergency of new pathogens. Microbial imbalances and unevenness have enhanced multiplication of pathogens and proliferation of many soil borne diseases, which are tedious to control. Biocontrols are promising and environmentally friendly agents against such diseases. However, microbiome composition and land use management have been mentioned as core factors in effectiveness of biocontrols. These factors are shaped by management and land use types. The focus of this research was to determine the diversity and abundance of microbes in the tomato rhizosphere and its effect on severity and control of bacterial wilt disease. Tomatoes were grown in a screen house at Namulonge using unsterilized soils from cultivated, fallow and forest land use types. Four biocontrols were used namely; Bacillus subtilis, Bacillus amyloliquefaciens, Peanibacillus polymyxa and Stenotrophomonas rhizophila. A negative control and a positive control were included. Data were collected on plant growth and yield parameters, and bacterial wilt disease incidence. Rhizosphere microbiome data were obtained using amplicon sequencing. Results showed that B. subtilis and P. polymyxa increased plant height in all land use types. Biocontrols B. amyloliquefaciens and S.rhizophila produced most dry biomass in cultivated land use, B. amyloliquefaciens and P. polymyxa in fallow and P. polymyxa and B. subtilis in forest land use type. In cultivated land use, biocontrols B. amyloliquefaciens, B. subtilis and S. rhizophila reduced bacterial wilt incidence by 31 % and P. polymyxa by 28 %. Fallow land use type had the highest microbial diversity and forest land use had the highest microbial abundance. The major phyla were Proteobacteria, Actinobacteria and Acidobacteria in Forest, Fallow and cultivated land uses respectively. Bacillus and Paenibacillus bacteria were more abundant in cultivated while Stenotrophomonas bacteria were more abundant in forest land use type.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationNampamya, D. (2019). Abundance and diversity of tomato rhizosphere microbes and their effect on bacterial wilt disease. Kampala, Uganda: Makerere University. 77p.en
dcterms.descriptionIITA supervisor: Dr. Danny Coyneen
dcterms.extent77p.en
dcterms.issued2019-12
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherMakerere Universityen
dcterms.subjectbiodiversityen
dcterms.subjectbiological controlen
dcterms.subjectpests of plantsen
dcterms.subjecttomatoesen
dcterms.subjectpathogensen
dcterms.subjectland useen
dcterms.subjectresearchen
dcterms.subjectbacteriaen
dcterms.subjectforestsen
dcterms.subjectagricultural productionen
dcterms.typeThesis

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