Molecular characterization of cowpea breeding lines for Striga resistance using SCAR markers

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationUniversity of Agriculture, Makurdien
cg.contributor.affiliationAhmadu Bello Universityen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversity of Virginiaen
cg.contributor.crpClimate Change, Agriculture and Food Security
cg.contributor.donorKirkhouse Trusten
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.howPublishedFormally Publisheden
cg.issn1939-1250en
cg.journalJournal of Agricultural Science and Technologyen
cg.reviewStatusPeer Reviewen
cg.subject.iitaCOWPEAen
dc.contributor.authorOmoigui, L.O.en
dc.contributor.authorYeye, M.en
dc.contributor.authorOusmane, B.en
dc.contributor.authorGowda, B.en
dc.contributor.authorTimko, M.P.en
dc.date.accessioned2017-05-03T08:45:49Zen
dc.date.available2017-05-03T08:45:49Zen
dc.identifier.urihttps://hdl.handle.net/10568/80871
dc.titleMolecular characterization of cowpea breeding lines for Striga resistance using SCAR markersen
dcterms.abstractS. gesnerioides (Willd) Vatke is a major biological constraint to cowpea production in the dry savanna of sub-Saharan Africa. Yield losses caused by S. gesnerioides in these regions are estimated in millions of tons annually, and prevalence of Striga soil infestation is steadily increasing. The availability of molecular markers tightly linked to S. gesnerioides resistance genes opens up the possibility of applying Marker-Assisted Selection (MAS) to cowpea and would fast track the process of developing resistance varieties to the parasite. In the present study, we report the use of Fast Technology for Analysis (FTA) also known as PlantSaver Cards (Whatman® FTA), developed by Flinder Technology associate to retrieve DNA from plant tissue for molecular analysis. A total of 100 F2 individual plants derived from two crosses were validated for SG3 resistance using two different SCAR markers (MahSe2 and C42B) linked to Striga race 3 (SG3) and 5 (SG5) resistance in other segregating populations. Genomic DNA was successfully recovered from leaf tissues of cowpea pressed onto FTA classic card and the DNA obtained from the FTA papers was found to be suitable for molecular analysis by PCR-based techniques. The marker efficiency of SCAR MahSe2 and C42B in detecting SG3 resistance was 98.5% and 93% respectively. This result revealed the utility of SCAR markers in cowpea breeding programme. Therefore, the application of MAS using FTA technology has the potential to increase efficiency of selection and for molecular characterization of cowpea lines for Striga resistance.en
dcterms.accessRightsLimited Access
dcterms.bibliographicCitationOmoigui, L., Yeye, M., Ousmane, B., Gowda, B. & Timko, M.P. (2012). Molecular characterization of cowpea breeding lines for striga resistance using SCAR markers. Journal of Agricultural Science and Technology B, 362-367.en
dcterms.descriptionEarlier title: Journal of Agricultural Science and Technology, ISSN 1939-1250 (DAVID PUBLISHING)en
dcterms.extent362-367en
dcterms.issued2012
dcterms.languageen
dcterms.subjectgenotypesen
dcterms.subjectyielden
dcterms.subjectcowpeasen
dcterms.subjectmarker assisted selectionen
dcterms.subjectraceen
dcterms.subjectfat technologyen
dcterms.typeJournal Article

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