Bean common mosaic : Screening for disease resistance

Morales, F.J. 1989. Bean common mosaic : Screening for disease resistance. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. 31 p. (CIAT Publication no. 33)

Methods used to identify BCMV are described in terms of symptomatology (common mosaic, black root); transmission (seed, insect, manual); responses of differential bean cv. (mosaic, systemic or local necrosis, local lesions, immunity); cytology (light and electron microscopy); purification and serology (double immunodiffusion test, enzyme-linked immunosorbent assay, immunosorbent electron microscopy); and BCMV characterization. Procedures in genetic improvement for resistance to BCMV include sources of resistance, incorporation of BCMV resistance to susceptible bean cv., multiple resistance to mosaic and necrosis- inducing BCMV strains, evaluation of germplasm for resistance, incorporation of the dominant necrosis (I) gene, screening for multiple resistance, and backcrossing. Production of BCMV-free seed, and seed germination and serological tests are presented. A flow chart outlining a general purification procedure for most BCMV strains is appended. Color photographs are included. (CIAT)