Prevalence and genome characterization of field isolates of sugarcane mosaic virus (SCMV) in Nigeria

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationAhmadu Bello Universityen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationTexas A&M Universityen
cg.contributor.crpMaize
cg.contributor.crpRoots, Tubers and Bananas
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.creator.identifierP. Lava Kumar: 0000-0003-4388-6510
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1094/pdis-08-18-1445-reen
cg.identifier.iitathemePLANT PRODUCTION & HEALTH
cg.isijournalISI Journalen
cg.issn0191-2917en
cg.issue5en
cg.journalPlant Diseaseen
cg.reviewStatusPeer Reviewen
cg.subject.iitaPLANT HEALTHen
cg.subject.iitaPLANT PRODUCTIONen
cg.volume103en
dc.contributor.authorYayaya, A.en
dc.contributor.authorDangora, D.B.en
dc.contributor.authorKumar, P. Lavaen
dc.contributor.authorAlegbejo, M.D.en
dc.contributor.authorGregg, L.en
dc.contributor.authorAlabi, O.J.en
dc.date.accessioned2018-11-13T09:10:43Zen
dc.date.available2018-11-13T09:10:43Zen
dc.identifier.urihttps://hdl.handle.net/10568/97926
dc.titlePrevalence and genome characterization of field isolates of sugarcane mosaic virus (SCMV) in Nigeriaen
dcterms.abstractMaize and sugarcane are two economically important crops often grown in adjacent fields or co-cultivated in the northern guinea savannah agroecological zone, a major cereal production region of Nigeria. This study was conducted to determine the prevalence of mosaic disease in sugarcane and maize crops in the northern guinea savannah agroecological zone and to molecularly characterize the associated sugarcane mosaic virus (SCMV, genus Potyvirus) isolates. Surveys were conducted from June to July 2015 and sugarcane mosaic disease (SCMD) incidence was assessed across 21 farmer’s fields. Mean SCMD incidence varied across states with ~82% (308/376), ~66% (143/218) and ~67% (36/54) recorded in Kaduna, Kano and Katsina states, respectively. RT-PCR analysis of 415 field-collected samples using genus-specific primers confirmed potyvirus infection in 63.7% (156/245) of sugarcane, 29.7% (42/141) of maize crops and 45% (13/29) itch grass samples. Cloning and sequencing of gene-specific DNA amplicons from a subset of 45 samples (sugarcane = 33, maize = 9, itch grass = 3) confirmed their specificities to SCMV. Phylogenetic analysis of the partial gene sequences showed that they all belong to a single monophyletic clade of SCMV. These results were supported by analysis of complete polyprotein sequences of representative maize and sugarcane isolates from Nigeria. Both isolates shared 94.9%/97.3% complete polyprotein nucleotide (nt)/amino acid (aa) identities with each other and 75.2%/97.6% nt/aa identities with corresponding sequences of global SCMV isolates. The detection of identical population of SCMV isolates in both crop species and a weed host suggests possible vector mediated interspecies spread within cereal landscapes in the study area with implications for the integrated and sustainable management of SCMD in cereal cropping systems in Nigeria.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationYahaya, A., Dangora, D.B., Kumar, P.L., Alegbejo, M., Gregg, L. & Alabi, O.J. (2019). Prevalence and genome characterization of field isolates of sugarcane mosaic virus (SCMV) in Nigeria. Plant Disease 103(5), 818-824.en
dcterms.extentp. 818-824en
dcterms.issued2019-05
dcterms.languageen
dcterms.licenseOther
dcterms.publisherScientific Societiesen
dcterms.subjectpotyvirusesen
dcterms.subjectcerealen
dcterms.subjectsugarcane mosaic virusen
dcterms.subjectepidemiologyen
dcterms.typeJournal Article

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