Comparison of serological and molecular methods for diagnosis of viruses causing yellowing and stunting symptoms for the pulse crops

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cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areasen_US
cg.contributor.affiliationAgricultural Research Corporationen_US
cg.contributor.affiliationDepartment of Agriculture and Fisheriesen_US
cg.contributor.donorArab Fund for Economic and Social Developmenten_US
cg.contributor.donorGrains Research and Development Corporationen_US
cg.contributor.donorDepartment of Agriculture and Fisheriesen_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.initiativePlant Healthen_US
cg.coverage.countryLebanonen_US
cg.coverage.iso3166-alpha2LBen_US
cg.coverage.regionWestern Asiaen_US
cg.creator.identifierAbdulrahman Moukahel: 0000-0003-4297-771Xen_US
cg.creator.identifierSafaa Kumari: 0000-0002-4492-6257en_US
cg.link.citationSafaa Kumari, Khaled Makkouk, Mejda Daami-Remadi, Asma Najar, Hajer Ben Ghanem, Asaad Nader, Mohammad Kassem, Abdulrahman Moukahel (editors). 2022. Abstracts book of the 13th Arab Congress of Plant Protection, Hammamet, Tunisia, 16-21 October 2022. Arab Journal of Plant Protection, 40 (Special issue), October 2022. 127 Pages (English Version).en_US
cg.subject.actionAreaResilient Agrifood Systemsen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 1 - No povertyen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
dc.contributor.authorMoukahel, Abdulrahmanen_US
dc.contributor.authorKumari, Safaaen_US
dc.contributor.authorHamed, Abdelmagid Adlanen_US
dc.contributor.authorSharman, Murrayen_US
dc.date.accessioned2023-01-17T20:47:59Zen_US
dc.date.available2023-01-17T20:47:59Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/127347en_US
dc.titleComparison of serological and molecular methods for diagnosis of viruses causing yellowing and stunting symptoms for the pulse cropsen_US
dcterms.abstractThe diagnosis of plant viruses that belong to the families Solemoviridae and Tombusviridae, which cause yellowing and stunting symptoms, is very complicated due to the serological cross-reactivity among these viruses. The main objective of this research was to study the efficiency of serological assays (tissue-blot immunoassay-TBIA) using specific monoclonal antibodies (MAbs) versus molecular assays (RT-PCR and sequencing) using generic and specific primer pairs. Legume samples (faba bean, chickpea and lentil) were collected from five different geographic regions: Ethiopia (11 samples), Lebanon (8 samples), Morocco (5 samples), Sudan (33 samples) and Uzbekistan (34 samples). All these samples reacted positively by TBIA with one or more MAbs and amplified by one or more primer pairs by RT-PCR. The comparison of serological with molecular results revealed the similarity between detection methods for both Bean leafroll virus (BLRV) and Soybean dwarf virus (SbDV) (both belong to genus Luteovirus, family Tombusviridae), as BLRV was detected in samples from Uzbekistan, and SbDV was detected in samples from Ethiopia and Uzbekistan only. On the other hand, the results of the serological and molecular methods for the virus species that belong to the genus Polerovirus (family Solemoviridae) were not comparable, especially in the samples collected from Sudan and Uzbekistan, as a number of samples collected from the five countries reacted positively with specific MAbs of Beet western yellows virus (BWYV) and Chickpea chlorotic stunt virus (CpCSV), but CpCSV and BWYV were identified only molecularly in the samples collected from Ethiopia, Lebanon and Morocco. Based on the nucleotide sequences obtained, new virus species that belong to the genus Polerovirus was identified in the samples collected from Sudan and Uzbekistan [e.g. Cucurbit aphid-borne yellows virus (CABYV) and Cotton leafroll dwarf virus (CLRDV)], and CpCSV was not identified molecularly in the samples from Uzbekistan or Sudan, in spite of the fact that a number of samples did react serologically with CpCSV MAb. The results obtained clearly showed that molecular assays are essential along with serological assays for more accurate and specific identification of Solemoviridae members, which is highly needed for the development of management strategies based on virus vector control and breeding for virus diseases resistance.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.available2022-10-16en_US
dcterms.bibliographicCitationMoukahel, A. R. S. G. Kumari, A. Adlan Hamed and M. Sharman. 2022. Comparison of serological and molecular methods for diagnosis of viruses causing yellowing and stunting symptoms for the pulse crops. In: Abstracts book of 13th Arab Congress of Plant Protection, Hammamet, Tunisia, 16-21 October 2022. S. G. Kumari, K. Makkouk, M. Daami-Remadi, A. Najar, H. Ben Ghanem, N. Asaad, M. Kassem and A. R. Moukahel (eds). Arab Journal of Plant Protection, 40 (Special issue), October 2022. Page E-64.en_US
dcterms.formatPDFen_US
dcterms.issued2022-10-16en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-4.0en_US
dcterms.publisherThe Arab Society for Plant Protectionen_US
dcterms.relationhttps://hdl.handle.net/20.500.11766/67949en_US
dcterms.relationhttps://hdl.handle.net/10568/127320en_US
dcterms.subjectmolecular characterizationen_US
dcterms.subjectfaba beansen_US
dcterms.subjectlentilsen_US
dcterms.subjectdetectionen_US
dcterms.subjectchickpeasen_US
dcterms.subjectserological diagnosisen_US
dcterms.subjectfaba beanen_US
dcterms.subjectlentilen_US
dcterms.subjectchickpeaen_US
dcterms.subjectyellowing virusesen_US
dcterms.subjectlegume viral diseasesen_US
dcterms.typeConference Paperen_US

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