Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationMinistry of Agriculture and Food Security, South Sudanen
cg.contributor.affiliationUniversity of Ghanaen
cg.contributor.affiliationInternational Crops Research Institute for the Semi-Arid Tropicsen
cg.contributor.affiliationInternational Maize and Wheat Improvement Centeren
cg.contributor.affiliationUniversity of Jubaen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationJohn Innes Centreen
cg.contributor.donorAfrica Biosciences Challenge Funden
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierOluwaseyi Shorinola: 0000-0003-3516-303X
cg.creator.identifierNasser K. Yao: 0000-0002-1183-708X
cg.creator.identifierPrasanna Boddupalli: 0000-0002-5761-2273
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fpls.2021.649308en
cg.isijournalISI Journalen
cg.issn1664-462Xen
cg.journalFrontiers in Plant Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.ilriCROPSen
cg.subject.ilriFOOD SECURITYen
cg.subject.ilriFOOD SYSTEMSen
cg.volume12en
dc.contributor.authorAwata, L.A.O.en
dc.contributor.authorIfie, B.E.en
dc.contributor.authorDanquah, E.en
dc.contributor.authorJumbo, M.B.en
dc.contributor.authorSuresh, L.M.en
dc.contributor.authorGowda, M.en
dc.contributor.authorMarchelo-Dragga, Philip W.en
dc.contributor.authorOlsen, M.S.en
dc.contributor.authorShorinola, Oluwaseyien
dc.contributor.authorYao, Nasseren
dc.contributor.authorBoddupalli, P.M.en
dc.contributor.authorTongoona, P.B.en
dc.date.accessioned2021-07-14T09:45:48Zen
dc.date.available2021-07-14T09:45:48Zen
dc.identifier.urihttps://hdl.handle.net/10568/114272
dc.titleIntrogression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenyaen
dcterms.abstractMaize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but can significantly be shortened when molecular tools are utilized in the breeding process. We used a donor parent (KS23-6) to transfer quantitative trait loci (QTL) for resistance to MLN into nine adapted but MLN susceptible lines. Nurseries were established in Kiboko, Kenya during 2015–2017 seasons and BC3F2 progeny were developed using marker assisted backcrossing (MABC) approach. Six single nucleotide polymorphism (SNP) markers linked to QTL for resistance to MLN were used to genotype 2,400 BC3F2 lines using Kompetitive Allele Specific PCR (KASP) platform. We detected that two of the six QTL had major effects for resistance to MLN under artificial inoculation field conditions in 56 candidate BC3F2 lines. To confirm whether these two QTL are reproducible under different field conditions, the 56 BC3F2 lines including their parents were evaluated in replicated trials for two seasons under artificial MLN inoculations in Naivasha, Kenya in 2018. Strong association of genotype with phenotype was detected. Consequently, 19 superior BC3F2 lines with favorable alleles and showing improved levels of resistance to MLN under artificial field inoculation were identified. These elite lines represent superior genetic resources for improvement of maize hybrids for resistance to MLN. However, 20 BC3F2 lines were fixed for both KASP markers but were susceptible to MLN under field conditions, which could suggest weak linkage between the KASP markers and target genes. The validated two major QTL can be utilized to speed up the breeding process but additional loci need to be identified between the KASP markers and the resistance genes to strengthen the linkage.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2021-05-03
dcterms.bibliographicCitationAwata, L.A.O., Ifie, B.E., Danquah, E., Jumbo, M.B., Suresh, L.M., Gowda, M., Marchelo-Dragga, P.W., Olsen, M.S., Shorinola, O., Yao, N.K., Boddupalli, P.M. and Tongoona, P.B. 2021. Introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in Naivasha, Kenya. Frontiers in Plant Science 12:649308.en
dcterms.issued2021-05-03
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Mediaen
dcterms.subjectcropsen
dcterms.subjectmaizeen
dcterms.subjectresearchen
dcterms.subjectfood systemsen
dcterms.typeJournal Article

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