A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisation

Simple item page
cg.authorship.typesCGIAR single centreen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.donorGatsby Charitable Foundationen
cg.coverage.countryBenin
cg.coverage.countryBurkina Faso
cg.coverage.countryCameroon
cg.coverage.countryCôte d'Ivoire
cg.coverage.countryGhana
cg.coverage.countryGuinea
cg.coverage.countryNigeria
cg.coverage.countryTogo
cg.coverage.iso3166-alpha2BJ
cg.coverage.iso3166-alpha2BF
cg.coverage.iso3166-alpha2CM
cg.coverage.iso3166-alpha2CI
cg.coverage.iso3166-alpha2GH
cg.coverage.iso3166-alpha2GN
cg.coverage.iso3166-alpha2NG
cg.coverage.iso3166-alpha2TG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.coverage.regionMiddle Africa
cg.identifier.doihttps://doi.org/10.1111/j.1744-7348.2003.tb00250.xen
cg.isijournalISI Journalen
cg.issn0003-4746en
cg.issue3en
cg.journalAnnals of Applied Biologyen
cg.reviewStatusPeer Reviewen
cg.subject.iitaYAMen
cg.subject.iitaPLANT GENETIC RESOURCESen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.volume142en
dc.contributor.authorMignouna, Hodeba D.en
dc.contributor.authorAbang, Mathew M.en
dc.contributor.authorFagbemi, S.A.en
dc.date.accessioned2018-08-09T06:40:30Zen
dc.date.available2018-08-09T06:40:30Zen
dc.identifier.urihttps://hdl.handle.net/10568/96346
dc.titleA comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterisationen
dcterms.abstractSeveral DNA-based marker systems are available for genetic fingerprinting of plants but information on their relative usefulness for yam germplasm characterisation is lacking. The efficiency of RAPD, AFLP and SSR markers for the assessment of genetic relationships, and for cultivar identification and discrimination among 45 West and Central African white yam cultivars belonging to 22 morphotypes/cultivar groups was investigated. Dendrograms were produced based on band pattern scores using the UPGMA method. Results showed that each of the three techniques could unequivocably identify each cultivar, but that techniques differed in the mean number of profiles generated per primer (or primer pair) per cultivar, referred to as genotype index (GI). The order of merit based on this criterion in this study was AFLPs (GI = 2.56), SSRs (GI = 0.39) and RAPDs (GI = 0.35). Yam genotypes classified in the same cultivar group based on morphology were often genetically different, emphasising the need for molecular fingerprinting in yam germplasm characterisation. AFLPs showed the highest efficiency in detecting polymorphism and revealed genetic relationships that most closely reflected morphological classification.en
dcterms.accessRightsLimited Access
dcterms.available2005-03-16
dcterms.bibliographicCitationMignouna, H.D., Abang, M.M. & Fagbemi, S.A. (2003). A comparative assessment of molecular marker assays (AFLP, RAPD and SSR) for white yam (Dioscorea rotundata) germplasm characterization. Annals of Applied Biology, 142(3), 269-276.en
dcterms.extentp. 269-276en
dcterms.issued2003-06
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWileyen
dcterms.subjectdioscorea rotundataen
dcterms.subjectgenetic variationen
dcterms.subjectgenotypesen
dcterms.subjectmolecular markersen
dcterms.subjectdnaen
dcterms.typeJournal Article

Files

Collections

IITA Journal Articles