Nitrate reductase activity in leaves as a plant physiological indicator of in vivo biological nitrification inhibition by Brachiaria humidicola

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cg.contributor.affiliationInternational Center for Tropical Agricultureen
cg.contributor.crpLivestock
cg.creator.identifierJacobo Arango: 0000-0002-4828-9398
cg.creator.identifierIdupulapati M. Rao: 0000-0002-8381-9358
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.plaphy.2019.02.002en
cg.identifier.projectCCAFS: PII-LAM_LivestockPlus
cg.isijournalISI Journalen
cg.issn0981-9428en
cg.journalPlant Physiology and Biochemistryen
cg.reviewStatusPeer Reviewen
cg.subject.ciatTROPICAL FORAGESen
cg.subject.ilriFORAGESen
cg.volume137en
dc.contributor.authorKarwat, Hannesen
dc.contributor.authorSparke, Marc-Andréen
dc.contributor.authorRasche, Franken
dc.contributor.authorArango, Jacoboen
dc.contributor.authorNuñez, Jonathanen
dc.contributor.authorRao, Idupulapati M.en
dc.contributor.authorMoreta, Daniloen
dc.contributor.authorCadisch, Georgen
dc.date.accessioned2019-02-14T19:06:33Zen
dc.date.available2019-02-14T19:06:33Zen
dc.identifier.urihttps://hdl.handle.net/10568/99438
dc.titleNitrate reductase activity in leaves as a plant physiological indicator of in vivo biological nitrification inhibition by Brachiaria humidicolaen
dcterms.abstractThe tropical forage grass Brachiaria humidicola (Bh) controls soil microbial nitrification via biological nitrification inhibition (BNI). The aim of our study was to verify if nitrate reductase activity (NRA) in Bh roots or leaves reflects in vivo performance of BNI in soils. NRA was measured in roots and leaves of contrasting accessions and apomictic hybrids of Bh grown under controlled greenhouse and natural field conditions. Nitrate (NO3−) contents were measured in soil solution and in Bh stem sap to validate NRA data. Potential soil nitrification rates (NRs) and leaf δ15N values were used to verify in vivo BNI by the NRA assay in the field study. NRA was detected in Bh leaves rather than roots, regardless of NO3− availability. NRA correlated with NO3− contents in soils and stem sap of contrasting Bh genotypes substantiating its reflectance of in vivo BNI performance. Additionally, leaf NRA data from the field study significantly correlated with simultaneously collected NRs and leaf δ15N data. The leaf NRA assay facilitated a rapid screening of contrasting Bh genotypes for their differences in in vivo performance of BNI under field and greenhouse conditions, but inconsistency of the BNI potential by Bh germplasm was observed. Among Bh genotypes tested, leaf NRA was closely linked with nitrification activity, and consequently with actual BNI performance. It was concluded that NRA in leaves of Bh can serve as an indicator of in vivo BNI activity when complemented with established BNI methodologies (δ15N, NRs) under greenhouse and field conditions.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationKarwat, Hannes; Sparke, Marc-André; Rasche, Frank; Arango, Jacobo; Nuñez, Jonathan; Rao, Idupulapati M.; Moreta, Danilo & Cadisch, Georg (2019). Nitrate reductase activity in leaves as a plant physiological indicator of in vivo biological nitrification inhibition by Brachiaria humidicola. Plant Physiology and Biochemistry, 137: 113-120 p.en
dcterms.extentp. 113-120en
dcterms.issued2019-04
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherElsevieren
dcterms.subjectfeed cropsen
dcterms.subjecttropical foragesen
dcterms.subjectbrachiariaen
dcterms.subjectnitrase assimilationen
dcterms.subjectasimilación de nitratosen
dcterms.subjectgermoplasmen
dcterms.subjectgenotypesen
dcterms.subjectgeneticsen
dcterms.subjectphysiologyen
dcterms.typeJournal Article

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