Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield

cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationMax-Planck-Institut für Molekulare Pflanzenphysiologieen
cg.contributor.affiliationUniversity of Kaiserslauternen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversity of Erlangen-Nurembergen
cg.contributor.affiliationBoyce Thompson Institute for Plant Researchen
cg.contributor.affiliationInstitute of Molecular Plant Biology, Switzerlanden
cg.contributor.affiliationInstitute for Biomedical Technologies, Italyen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorBill & Melinda Gates Foundationen
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.creator.identifierANDREAS GISEL: 0000-0001-7218-9488
cg.creator.identifierLIVIA STAVOLONE: 0000-0002-0691-1302
cg.creator.identifierLukas Mueller: 0000-0001-8640-1750
cg.creator.identifierUwe Sonnewald: 0000-0003-1835-5339
cg.creator.identifierAlisdair Fernie: 0000-0001-9000-335X
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1111/tpj.14693en
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journalen
cg.issn0960-7412en
cg.issue6en
cg.journalPlant Journalen
cg.reviewStatusPeer Reviewen
cg.subject.iitaAGRONOMYen
cg.subject.iitaCASSAVAen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT PRODUCTIONen
cg.volume102en
dc.contributor.authorObata, T.en
dc.contributor.authorKlemens, P.A.W.en
dc.contributor.authorRosado Souza, L.en
dc.contributor.authorSchlereth, A.en
dc.contributor.authorGisel, A.en
dc.contributor.authorStavolone, L.en
dc.contributor.authorZierer, W.en
dc.contributor.authorMorales, N.en
dc.contributor.authorMuller, L.A.en
dc.contributor.authorZeeman, S.C.en
dc.contributor.authorLudewig, F.en
dc.contributor.authorStitt, M.en
dc.contributor.authorSonnewald, U.en
dc.contributor.authorNeuhaus, H.E.en
dc.contributor.authorFernie, A.R.en
dc.date.accessioned2020-03-17T15:29:58Zen
dc.date.available2020-03-17T15:29:58Zen
dc.identifier.urihttps://hdl.handle.net/10568/107786
dc.titleMetabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yielden
dcterms.abstractCassava is an important staple crop in sub‐Saharan Africa, due to its high productivity even on nutrient poor soils. The metabolic characteristics underlying this high productivity are poorly understood including the mode of photosynthesis, reasons for the high rate of photosynthesis, the extent of source/sink limitation, the impact of environment, and the extent of variation between cultivars. Six commercial African cassava cultivars were grown in a greenhouse in Erlangen, Germany, and in the field in Ibadan, Nigeria. Source leaves, sink leaves, stems and storage roots were harvested during storage root bulking and analyzed for sugars, organic acids, amino acids, phosphorylated intermediates, minerals, starch, protein, activities of enzymes in central metabolism and yield traits. High ratios of RuBisCO:phosphoenolpyruvate carboxylase activity support a C3 mode of photosynthesis. The high rate of photosynthesis is likely to be attributed to high activities of enzymes in the Calvin–Benson cycle and pathways for sucrose and starch synthesis. Nevertheless, source limitation is indicated because root yield traits correlated with metabolic traits in leaves rather than in the stem or storage roots. This situation was especially so in greenhouse‐grown plants, where irradiance will have been low. In the field, plants produced more storage roots. This was associated with higher AGPase activity and lower sucrose in the roots, indicating that feedforward loops enhanced sink capacity in the high light and low nitrogen environment in the field. Overall, these results indicated that carbon assimilation rate, the K battery, root starch synthesis, trehalose, and chlorogenic acid accumulation are potential target traits for genetic improvement.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2020-03-04
dcterms.bibliographicCitationObata, T., Klemens, P.A., Rosado‐Souza, L., Schlereth, A., Gisel, A., Stavolone, L., ... & Fernie, A.R. (2020). Metabolic profiles of six African cultivars of cassava (Manihot esculenta Crantz) highlight bottlenecks of root yield. The Plant Journal, 1-40.en
dcterms.extentp. 1202-1219en
dcterms.issued2020-06
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherWileyen
dcterms.subjectcassavaen
dcterms.subjectyieldsen
dcterms.subjectphotosynthesisen
dcterms.subjectcarbonen
dcterms.subjectenzyme activityen
dcterms.subjectnitrogen metabolismen
dcterms.subjectstarchen
dcterms.subjectcell biologyen
dcterms.subjectgeneticsen
dcterms.typeJournal Article

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