Molecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeria

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationUniversity of Bonnen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversity of Arizonaen
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1080/19440040903551954en
cg.isijournalISI Journalen
cg.issn1944-0049en
cg.issue5en
cg.journalFood Additives and Contaminantsen
cg.reviewStatusPeer Reviewen
cg.subject.iitaAFLATOXINen
cg.subject.iitaDISEASE CONTROLen
cg.subject.iitaFOOD SECURITYen
cg.volume27en
dc.contributor.authorDonner, M.en
dc.contributor.authorAtehnkeng, J.en
dc.contributor.authorSikora, R.A.en
dc.contributor.authorBandyopadhyay, Ranajiten
dc.contributor.authorCotty, P.J.en
dc.date.accessioned2017-09-21T14:08:05Zen
dc.date.available2017-09-21T14:08:05Zen
dc.identifier.urihttps://hdl.handle.net/10568/87993
dc.titleMolecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeriaen
dcterms.abstractAbstract Aflatoxins are highly toxic carcinogens produced by several species in Aspergillus section Flavi. Strains of A. flavus that do not produce aflatoxins, called atoxigenic strains, have been used commercially in North America as tools for limiting aflatoxin contamination. A similar aflatoxin management strategy is being pursued in Nigeria. In the current study, loci across the 68 kb aflatoxin biosynthesis gene cluster were compared among 18 atoxigenic and two aflatoxin-producing vegetative compatibility groups (VCGs) from Nigeria and an atoxigenic VCG used commercially in North America. Five of the atoxigenic VCGs had large deletions (37–65 kb) extending from the teleomeric side of the aflatoxin biosynthesis cluster. In one VCG (AV0222) the deletion extended through the cluster to the adjacent sugar cluster. The remaining twelve atoxigenic VCGs, including the VCG used for aflatoxin management in North America, contained all the aflatoxin pathway genes, but with defects. Two observations support the long-term persistence of atoxigenicity within A. flavus: first, a comparison of pathway genes revealed more changes in atoxigenic than in aflatoxin-producing isolates relative to the aflatoxin-producing strain NRRL 3357; and second, several non-synonymous changes are unique to atoxigenics. Atoxigenic VCG diversity was assessed with phylogenetic analyses. Although some atoxigenics share relatively recent ancestry, several are more closely related to aflatoxin producers than to other atoxigenics. The current study demonstrates VCGs of A. flavus in West Africa with diverse mechanisms of atoxigenicity and potential value in aflatoxin management programmes.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.available2010-05-07
dcterms.bibliographicCitationDonner, M., Atehnkeng, J., Sikora, R.A., Bandyopadhyay, R. & Cotty, P.J. (2010). Molecular characterization of atoxigenic strains for biological control of aflatoxins in Nigeria. Food Additives and Contaminants, 27(5), 576-590.en
dcterms.extent576-590en
dcterms.issued2010-05
dcterms.languageen
dcterms.publisherInforma UK Limiteden
dcterms.subjectpolymerase chain reactionen
dcterms.subjectmycologyen
dcterms.subjectaflatoxinsen
dcterms.subjectcerealsen
dcterms.subjecthealth significanceen
dcterms.subjectaspergillus flavien
dcterms.subjectatoxigenic strainsen
dcterms.subjectvegetative compatibility groupsen
dcterms.subjecttoxicologyen
dcterms.subjectfood scienceen
dcterms.typeJournal Article

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