Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Center for Tropical Agricultureen
cg.contributor.affiliationFrench National Research Institute for Sustainable Developmenten
cg.contributor.affiliationUniversidad Nacional de Colombiaen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeGenome Editing
cg.coverage.countryColombia
cg.coverage.iso3166-alpha2CO
cg.coverage.regionAmericas
cg.coverage.regionSouth America
cg.coverage.regionLatin America and the Caribbean
cg.creator.identifierFrancisco Sanchez: 0000-0001-5716-8945
cg.creator.identifierPaul Chavarriaga-Aguirre: 0000-0001-7579-3250
cg.subject.actionAreaGenetic Innovation
cg.subject.alliancebiovciatAGRICULTUREen
cg.subject.alliancebiovciatCASSAVAen
cg.subject.impactAreaClimate adaptation and mitigation
cg.subject.sdgSDG 13 - Climate actionen
dc.contributor.authorSanchez, Francisco J.en
dc.contributor.authorZárate, Carlos A.en
dc.contributor.authorSzurek, Borisen
dc.contributor.authorDíaz, Paula A.en
dc.contributor.authorChavarriaga, Paulen
dc.date.accessioned2024-11-11T09:31:13Zen
dc.date.available2024-11-11T09:31:13Zen
dc.identifier.urihttps://hdl.handle.net/10568/159487
dc.titleEditing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024en
dcterms.abstractCassava (Manihot esculenta Crantz) is considered the third most important crop globally. The mitigation of diseases like cassava bacterial blight (CBB), caused by the phytopathogenic bacterium Xanthomonas phaseoli pv. manihotis (Xpm) is key for the success of the crop. CBB leads to significant yield losses, ranging from 12% to 100%. One of the infection mechanisms employed by Xpm involves TALE-type proteins, which facilitate bacterial proliferation and the onset of disease symptoms. It has been demonstrated that virulence mechanisms activate certain gene families, including the SWEET gene family, which encodes sugar transporters (such as glucose and sucrose) that provide a carbon source for the bacteria to grow a cause infection. The aim of our research is to assess the resistance of cassava lines edited in the MeSweet 10a and 10e genes using CRISPR/Cas9 to confer resistance to CBB in the model variety 60444, susceptible to CBB. The goal is to evaluate the impact of editions in the promoter or coding regions for resistance to Xpm infection. Lines exhibiting the most promising mutations (INDELS) in targeted regions will be identified through molecular assays, whichthen will be established under in vitro and greenhouse conditions to be infected with Xpm strains and evaluatedisease resistance.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationSanchez, F.J.; Zárate, C.A.; Szurek, B.; Díaz, P.A.; Chavarriaga, P. (2024) Editing cassava sweet genes for resistance to Xanthomonas. Reporting period: Second Semester 2024. 12 p.en
dcterms.extent12 p.en
dcterms.issued2024-10
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.subjectcassavaen
dcterms.subjectgene editingen
dcterms.subjectcrispren
dcterms.typeReport

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