Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationChitipa District Council, Malawien
cg.contributor.affiliationLilongwe University of Agriculture and Natural Resourcesen
cg.contributor.affiliationCentral Veterinary Laboratory, Malawien
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationUniversity of Edinburghen
cg.contributor.affiliationHokkaido Universityen
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen
cg.contributor.donorJapan Society for the Promotion of Scienceen
cg.coverage.countryMalawi
cg.coverage.iso3166-alpha2MW
cg.coverage.regionAfrica
cg.coverage.regionSouthern Africa
cg.creator.identifierLian Thomas: 0000-0001-8447-1210
cg.creator.identifierFlorence Mutua: 0000-0002-1007-5511
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1007/s11259-025-10717-9en
cg.isijournalISI Journalen
cg.issn0165-7380en
cg.issue3en
cg.journalVeterinary Research Communicationsen
cg.reviewStatusPeer Reviewen
cg.speciesFasciola giganticaen
cg.subject.ilriANIMAL HEALTHen
cg.subject.ilriCATTLEen
cg.subject.ilriEPIDEMIOLOGYen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume49en
dc.contributor.authorMogha, L.en
dc.contributor.authorKainga, H.en
dc.contributor.authorKamanga, N.en
dc.contributor.authorKapalamula, T.F.en
dc.contributor.authorWood, C.en
dc.contributor.authorThomas, Lian F.en
dc.contributor.authorMutua, Florence K.en
dc.contributor.authorSargison, N.en
dc.contributor.authorHayashida, K.en
dc.contributor.authorTsutsumi, T.en
dc.contributor.authorHayashi, N.en
dc.contributor.authorNonaka, N.en
dc.contributor.authorNakao, R.en
dc.contributor.authorChatanga, E.en
dc.date.accessioned2025-04-04T12:25:55Zen
dc.date.available2025-04-04T12:25:55Zen
dc.identifier.urihttps://hdl.handle.net/10568/174013
dc.titleGenetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawien
dcterms.abstract<i>Fasciola gigantica</i> is an important trematode that affects the health of animals and humans in tropical and subtropical countries, including Malawi. Information on the genetic diversity and population structure of <i>F. gigantica</i> is important to understanding the parasite's transmission patterns/ and in monitoring the development of resistance to commonly used anthelmintic agents. This study aimed to analyze the genetic diversity and population structure of <i>Fasciola</i> species collected from cattle at slaughter slabs and abattoirs in selected districts of Malawi. A total of 27 adult liver flukes were collected from cattle at slaughter slabs and abattoirs in the northern region (n = 12), central region (n = 5), and southern region (n = 10). The mitochondrial cytochrome c oxidase I (COI) gene and nicotinamide adenine dinucleotide dehydrogenase 1 (ND1) gene were amplified and the amplicons were sequenced for all samples. The sequences obtained were used to investigate genetic diversity through median-joining networks and phylogenetic analysis. Tajima’s D test and Fu’s Fs statistics were used to determine the population structure. Based on the analyzed COI and ND1 sequences, all samples were identified as <i>F. gigantica</i>. Single nucleotide polymorphisms (SNPs) were identified at 18 and 17 positions for COI and ND1 genes, resulting in 10 and 5 haplotypes, respectively. The haplotype diversities were 0.867 and 0.479 for COI and ND1 gene sequences, respectively. The population genetic structure indices showed a population that has undergone a recent expansion. This study provides baseline epidemiological data on the genetic diversity and population structure of <i>F. gigantica</i> in Malawi; which is important for its control.en
dcterms.accessRightsLimited Access
dcterms.audienceAcademicsen
dcterms.audienceScientistsen
dcterms.available2025-04-01
dcterms.bibliographicCitationMogha, L., Kainga, H., Kamanga, N., Kapalamula, T.F., Wood, C., Thomas, L.F., Mutua, F., Sargison, N., Hayashida, K., Tsutsumi, T., Hayashi, N., Nonaka, N., Nakao, R. and Chatanga, E. 2025. Genetic diversity and population structure of <i>Fasciola gigantica</i> isolated from cattle in Malawi. Veterinary Research Communications 49(3): 157.en
dcterms.extent157en
dcterms.issued2025-04-01
dcterms.languageen
dcterms.licenseOther
dcterms.publisherSpringeren
dcterms.subjectanimal healthen
dcterms.subjectcattleen
dcterms.subjectepidemiologyen
dcterms.subjectparasitesen
dcterms.typeJournal Article

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