Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationChitipa District Council, Malawien_US
cg.contributor.affiliationLilongwe University of Agriculture and Natural Resourcesen_US
cg.contributor.affiliationCentral Veterinary Laboratory, Malawien_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationUniversity of Edinburghen_US
cg.contributor.affiliationHokkaido Universityen_US
cg.contributor.donorFederal Ministry for Economic Cooperation and Development, Germanyen_US
cg.contributor.donorJapan Society for the Promotion of Scienceen_US
cg.coverage.countryMalawien_US
cg.coverage.iso3166-alpha2MWen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionSouthern Africaen_US
cg.creator.identifierLian Thomas: 0000-0001-8447-1210en_US
cg.creator.identifierFlorence Mutua: 0000-0002-1007-5511en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1007/s11259-025-10717-9en_US
cg.isijournalISI Journalen_US
cg.issn0165-7380en_US
cg.issue3en_US
cg.journalVeterinary Research Communicationsen_US
cg.reviewStatusPeer Reviewen_US
cg.speciesFasciola giganticaen_US
cg.subject.ilriANIMAL HEALTHen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriEPIDEMIOLOGYen_US
cg.subject.impactAreaNutrition, health and food securityen_US
cg.subject.sdgSDG 2 - Zero hungeren_US
cg.volume49en_US
dc.contributor.authorMogha, L.en_US
dc.contributor.authorKainga, H.en_US
dc.contributor.authorKamanga, N.en_US
dc.contributor.authorKapalamula, T.F.en_US
dc.contributor.authorWood, C.en_US
dc.contributor.authorThomas, Lian F.en_US
dc.contributor.authorMutua, Florence K.en_US
dc.contributor.authorSargison, N.en_US
dc.contributor.authorHayashida, K.en_US
dc.contributor.authorTsutsumi, T.en_US
dc.contributor.authorHayashi, N.en_US
dc.contributor.authorNonaka, N.en_US
dc.contributor.authorNakao, R.en_US
dc.contributor.authorChatanga, E.en_US
dc.date.accessioned2025-04-04T12:25:55Zen_US
dc.date.available2025-04-04T12:25:55Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/174013en_US
dc.titleGenetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawien_US
dcterms.abstract<i>Fasciola gigantica</i> is an important trematode that affects the health of animals and humans in tropical and subtropical countries, including Malawi. Information on the genetic diversity and population structure of <i>F. gigantica</i> is important to understanding the parasite's transmission patterns/ and in monitoring the development of resistance to commonly used anthelmintic agents. This study aimed to analyze the genetic diversity and population structure of <i>Fasciola</i> species collected from cattle at slaughter slabs and abattoirs in selected districts of Malawi. A total of 27 adult liver flukes were collected from cattle at slaughter slabs and abattoirs in the northern region (n = 12), central region (n = 5), and southern region (n = 10). The mitochondrial cytochrome c oxidase I (COI) gene and nicotinamide adenine dinucleotide dehydrogenase 1 (ND1) gene were amplified and the amplicons were sequenced for all samples. The sequences obtained were used to investigate genetic diversity through median-joining networks and phylogenetic analysis. Tajima’s D test and Fu’s Fs statistics were used to determine the population structure. Based on the analyzed COI and ND1 sequences, all samples were identified as <i>F. gigantica</i>. Single nucleotide polymorphisms (SNPs) were identified at 18 and 17 positions for COI and ND1 genes, resulting in 10 and 5 haplotypes, respectively. The haplotype diversities were 0.867 and 0.479 for COI and ND1 gene sequences, respectively. The population genetic structure indices showed a population that has undergone a recent expansion. This study provides baseline epidemiological data on the genetic diversity and population structure of <i>F. gigantica</i> in Malawi; which is important for its control.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.audienceAcademicsen_US
dcterms.audienceScientistsen_US
dcterms.available2025-04-01en_US
dcterms.bibliographicCitationMogha, L., Kainga, H., Kamanga, N., Kapalamula, T.F., Wood, C., Thomas, L.F., Mutua, F., Sargison, N., Hayashida, K., Tsutsumi, T., Hayashi, N., Nonaka, N., Nakao, R. and Chatanga, E. 2025. Genetic diversity and population structure of <i>Fasciola gigantica</i> isolated from cattle in Malawi. Veterinary Research Communications 49(3): 157.en_US
dcterms.extent157en_US
dcterms.issued2025-04-01en_US
dcterms.languageenen_US
dcterms.licenseOtheren_US
dcterms.publisherSpringeren_US
dcterms.subjectanimal healthen_US
dcterms.subjectcattleen_US
dcterms.subjectepidemiologyen_US
dcterms.subjectparasitesen_US
dcterms.typeJournal Articleen_US

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