Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm

Simple item page
cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationNational Crops Resources Research Institute, Ugandaen
cg.contributor.affiliationMakerere Universityen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeAccelerated Breeding
cg.coverage.countryUganda
cg.coverage.iso3166-alpha2UG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierIsmail Rabbi: 0000-0001-9966-2941
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fpls.2022.1017275en
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journalen
cg.issn1664-462Xen
cg.issue1017275en
cg.journalFrontiers in Plant Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.iitaAGRONOMYen
cg.subject.iitaCASSAVAen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 1 - No povertyen
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume13en
dc.contributor.authorEsuma, W.en
dc.contributor.authorEyoo, O.en
dc.contributor.authorGwandu, F.en
dc.contributor.authorMukasa, S.en
dc.contributor.authorAlicai, Titusen
dc.contributor.authorOzimati, A.en
dc.contributor.authorNuwamanya, E.en
dc.contributor.authorRabbi, I.Y.en
dc.contributor.authorKawuki, R.S.en
dc.date.accessioned2023-01-18T12:01:37Zen
dc.date.available2023-01-18T12:01:37Zen
dc.identifier.urihttps://hdl.handle.net/10568/127385
dc.titleValidation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasmen
dcterms.abstractIntroduction The intrinsic high heterozygosity of cassava makes conventional breeding ineffective for rapid genetic improvement. However, recent advances in next-generation sequencing technologies have enabled the use of high-density markers for genome-wide association studies, aimed at identifying single nucleotide polymorphisms (SNPs) linked to major traits such as cassava mosaic disease (CMD) resistance, dry matter content (DMC) and total carotenoids content (TCC). A number of these trait-linked SNPs have been converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers for downstream application of marker assisted selection. Methods We assayed 13 KASP markers to evaluate their effectiveness in selecting for CMD, DMC and TCC in 1,677 diverse cassava genotypes representing two independent breeding populations in Uganda. Results Five KASP markers had significant co-segregation with phenotypes; CMD resistance (2), DMC (1) and TCC (2), with each marker accounting for at least 30% of the phenotypic variation. Markers located within the chromosomal regions for which strong marker-trait association loci have been characterised (chromosome 12 markers for CMD, chromosome 1 markers for DMC and TCC) had consistently superior ability to discriminate the respective phenotypes. Discussion The results indicate varying discriminatory abilities of the KASP markers assayed and the need for their context-based use for MAS, with PSY2_572 particularly effective in selecting for high TCC. Availing the effective KASP markers on cost-effective genotyping platforms could facilitate practical implementation of marker-assisted cassava breeding for accelerated genetic gains for CMD, DMC and provitamin A carotenoids.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2022-11-23
dcterms.bibliographicCitationEsuma, W., Eyoo, O., Gwandu, F., Mukasa, S., Alicai, T., Ozimati, A., ... & Kawuki, R. (2022). Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm. Frontiers in Plant Science, 13: 1017275, 1-12.en
dcterms.extent1-12en
dcterms.issued2022-11-23
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherFrontiers Mediaen
dcterms.subjectgenetic gainen
dcterms.subjectcassavaen
dcterms.subjectmanihot esculentaen
dcterms.subjectgenetic markersen
dcterms.subjectgenotypesen
dcterms.subjectugandaen
dcterms.typeJournal Article

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Validation of KASP markers....pdf
Size:
2.3 MB
Format:
Adobe Portable Document Format
Description:
Journal Article
Download

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

IITA Journal Articles
CGIAR Initiative on Accelerated Breeding