Genetic diversity and symbiotic effectiveness of Bradyrhizobium strains nodulating selected annual grain legumes growing in Ethiopia

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationAdama Science and Technology Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationUniversität Hohenheimen
cg.contributor.crpLivestock
cg.coverage.countryEthiopia
cg.coverage.iso3166-alpha2ET
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierEndalkachew Wolde-meskel: 0000-0001-6433-0162en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1099/ijsem.0.002486en
cg.isijournalISI Journalen
cg.issn1466-5026en
cg.issue1en
cg.journalInternational Journal of Systematic and Evolutionary Microbiologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL FEEDINGen
cg.subject.ilriCROP-LIVESTOCKen
cg.subject.ilriFEEDSen
cg.subject.ilriLEGUMESen
cg.subject.ilriRESEARCHen
cg.volume68en
dc.contributor.authorDegefu, T.en
dc.contributor.authorWoldemeskel, Endalkachewen
dc.contributor.authorRasche, F.en
dc.date.accessioned2018-03-20T13:39:26Zen
dc.date.available2018-03-20T13:39:26Zen
dc.identifier.urihttps://hdl.handle.net/10568/91711
dc.titleGenetic diversity and symbiotic effectiveness of Bradyrhizobium strains nodulating selected annual grain legumes growing in Ethiopiaen
dcterms.abstractVigna unguiculata, Vigna radiata and Arachis hypogaea growing in Ethiopia are nodulated by a genetically diverse group of Bradyrhizobium strains. To determine the genetic identity and symbiotic effectiveness of these bacteria, a collection of 36 test strains originating from the root nodules of the three hosts was investigated using multilocus sequence analyses (MLSA) of core genes including 16S rRNA, recA, glnII, gyrB, atpD and dnaK. Sequence analysis of nodA and nifH genes along with tests for symbiotic effectiveness using δ15N analysis were also carried out. The phylogenetic trees derived from the MLSA grouped most test strains into four well-supported distinct positions designated as genospecies I–IV. The maximum likelihood (ML) tree that was constructed based on the nodA gene sequences separated the entire test strains into two lineages, where the majority of the test strains were clustered on one of a well-supported large branch that comprise Bradyrhizobium species from the tropics. This clearly suggested the monophyletic origin of the nodA genes within the bradyrhizobia of tropical origin. The δ15N-based symbiotic effectiveness test of seven selected strains revealed that strains GN100 (δ15 N=0.73) and GN102 (δ15 N=0.79) were highly effective nitrogen fixers when inoculated to cowpea, thus can be considered as inoculants in cowpea production. It was concluded that Ethiopian soils are a hotspot for rhizobial diversity. This calls for further research to unravel as yet unknown bradyrhizobia nodulating legume host species growing in the country. In this respect, prospective research should also address the mechanisms of symbiotic specificity that could lead to high nitrogen fixation in target legumes.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationDegefu, T., Wolde-meskel, E. and Rasche, F. 2018. Genetic diversity and symbiotic effectiveness of Bradyrhizobium strains nodulating selected annual grain legumes growing in Ethiopia. International Journal of Systematic and Evolutionary Microbiology 68: 449-460en
dcterms.extentp. 449-460en
dcterms.issued2018-01-01en
dcterms.languageen
dcterms.publisherMicrobiology Societyen
dcterms.subjectfeedsen
dcterms.subjectresearchen
dcterms.subjectbradyrhizobiumen
dcterms.subjectlegumesen
dcterms.subjectmicrobiologyen
dcterms.typeJournal Article

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