A milestone in the doubled haploid pathway of cassava

Simple item page
cg.creator.identifierLuis Augusto Becerra Lopez-Lavalle: 0000-0003-3520-2270
cg.identifier.doihttps://doi.org/10.1007/s00709-013-0543-6en
cg.isijournalISI Journalen
cg.issn0033-183Xen
cg.issue1en
cg.journalProtoplasmaen
cg.subject.ciatCASSAVAen
cg.volume251en
dc.contributor.authorPerera, Prasanthi I.P.en
dc.contributor.authorOrdoñez, C.A.en
dc.contributor.authorBecerra López Lavelle, Luis Augustoen
dc.contributor.authorDedicova, Beataen
dc.date.accessioned2014-09-09T16:58:07Zen
dc.date.available2014-09-09T16:58:07Zen
dc.identifier.urihttps://hdl.handle.net/10568/42276
dc.titleA milestone in the doubled haploid pathway of cassavaen
dcterms.abstractThis study was aimed at inducing androgenesis in cultured anthers of cassava (Manihot esculenta Crantz) to develop a protocol for the production of doubled haploids. Microspore reprogramming was induced in cassava by cold or heat stress of anthers. Since the anthers contain both haploid microspores and diploid somatic cells, it was essential to verify the origin of anther-derived calli. The origin of anther-derived calli was assessed by morphological screening followed by histological analysis and flow cytometry (FCM). Additionally, simple sequence repeat (SSR) and amplified fragmented length polymorphism (AFLP) assays were used for the molecular identification of the microspore-derived calli. The study clearly demonstrated the feasibility of producing microspore-derived calli using heat- or cold-pretreated anthers. Histological studies revealed reprogramming of the developmental pathway of microspores by symmetrical division of the nucleus. Flow cytometry analysis revealed different ploidy level cell types including haploids, which confirmed their origin from the microspores. The SSR and AFLP marker assays independently confirmed the histological and FCM results of a haploid origin of the calli at the DNA level. The presence of multicellular microspores in the in vitro system indicated a switch of developmental program, which constitutes a crucial step in the design of protocols for the regeneration of microspore-derived embryos and plants. This is the first detailed report of calli, embryos, and abnormal shoots originated from the haploid cells in cassava, leading to the development of a protocol for the production of doubled haploid plants in cassava.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2013-09-13
dcterms.bibliographicCitationPerera, PIP; Ordoñez, CA; Becerra López-Lavalle, Luis Augusto; Dedicova, Beata. 2014. A milestone in the doubled haploid pathway of cassava. Protoplasma 251(1): 233-246.en
dcterms.extentpp. 233-246en
dcterms.issued2014-01
dcterms.languageen
dcterms.licenseCC-BY-NC-2.0
dcterms.publisherSpringeren
dcterms.subjectmanihot esculentaen
dcterms.subjectanther cultureen
dcterms.subjectandrogenesisen
dcterms.subjectamplified fragment length polymorphismen
dcterms.subjectmicrosatellitesen
dcterms.subjecthistologyen
dcterms.subjectcultivo de anterasen
dcterms.subjectandrogénesisen
dcterms.subjectpolimorfismo de longitud de fragmentos amplificadosen
dcterms.subjectmicrosatélitesen
dcterms.subjecthistologíaen
dcterms.subjectcell biologyen
dcterms.typeJournal Article

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIAT Articles in Journals
CIAT Agrobiodiversity