The wheat durable, multipathogen resistance gene Lr34 confers partial blast resistance in rice

cg.contributor.affiliationUniversity of Zürichen
cg.contributor.affiliationInternational Rice Research Instituteen
cg.contributor.affiliationZhejiang Academy of Agricultural Sciencesen
cg.contributor.affiliationCommonwealth Scientific and Industrial Research Organizationen
cg.contributor.affiliationCentre de Coopération Internationale en Recherche Agronomique pour le Dévelopmenten
cg.contributor.affiliationAachen Universityen
cg.identifier.doihttps://doi.org/10.1111/pbi.12491en
cg.issn1467-7644en
cg.issue5en
cg.journalPlant Biotechnology Journalen
cg.volume14en
dc.contributor.authorKrattinger, Simon G.en
dc.contributor.authorSucher, Justineen
dc.contributor.authorSelter, Liselotte L.en
dc.contributor.authorChauhan, Harshen
dc.contributor.authorZhou, Boen
dc.contributor.authorTang, Mingzhien
dc.contributor.authorUpadhyaya, Narayana M.en
dc.contributor.authorMieulet, Delphineen
dc.contributor.authorGuiderdoni, Emmanuelen
dc.contributor.authorWeidenbach, Deniseen
dc.contributor.authorSchaffrath, Ulrichen
dc.contributor.authorLagudah, Evans S.en
dc.contributor.authorKeller, Beaten
dc.date.accessioned2024-12-19T12:55:02Zen
dc.date.available2024-12-19T12:55:02Zen
dc.identifier.urihttps://hdl.handle.net/10568/165383
dc.titleThe wheat durable, multipathogen resistance gene Lr34 confers partial blast resistance in riceen
dcterms.abstractThe wheat gene Lr34 confers durable and partial field resistance against the obligate biotrophic, pathogenic rust fungi and powdery mildew in adult wheat plants. The resistant Lr34 allele evolved after wheat domestication through two gain‐of‐function mutations in an ATP‐binding cassette transporter gene. An Lr34‐like fungal disease resistance with a similar broad‐spectrum specificity and durability has not been described in other cereals. Here, we transformed the resistant Lr34 allele into the japonica rice cultivar Nipponbare. Transgenic rice plants expressing Lr34 showed increased resistance against multiple isolates of the hemibiotrophic pathogen Magnaporthe oryzae, the causal agent of rice blast disease. Host cell invasion during the biotrophic growth phase of rice blast was delayed in Lr34‐expressing rice plants, resulting in smaller necrotic lesions on leaves. Lines with Lr34 also developed a typical, senescence‐based leaf tip necrosis (LTN) phenotype. Development of LTN during early seedling growth had a negative impact on formation of axillary shoots and spikelets in some transgenic lines. One transgenic line developed LTN only at adult plant stage which was correlated with lower Lr34 expression levels at seedling stage. This line showed normal tiller formation and more importantly, disease resistance in this particular line was not compromised. Interestingly, Lr34 in rice is effective against a hemibiotrophic pathogen with a lifestyle and infection strategy that is different from obligate biotrophic rusts and mildew fungi. Lr34 might therefore be used as a source in rice breeding to improve broad‐spectrum disease resistance against the most devastating fungal disease of rice.en
dcterms.available2015-10-15
dcterms.bibliographicCitationKrattinger, Simon G.; Sucher, Justine; Selter, Liselotte L.; Chauhan, Harsh; Zhou, Bo; Tang, Mingzhi; Upadhyaya, Narayana M.; Mieulet, Delphine; Guiderdoni, Emmanuel; Weidenbach, Denise; Schaffrath, Ulrich; Lagudah, Evans S. and Keller, Beat. 2016. The wheat durable, multipathogen resistance gene Lr34 confers partial blast resistance in rice. Plant Biotechnology Journal, Volume 14 no. 5 p. 1261-1268en
dcterms.extentpp. 1261-1268en
dcterms.issued2016-05
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWileyen
dcterms.typeJournal Article

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