Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves

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cg.contributor.affiliationWashington State Universityen
cg.contributor.affiliationInternational Rice Research Instituteen
cg.contributor.affiliationUniversity of Cambridgeen
cg.contributor.affiliationRussian Academy of Sciencesen
cg.contributor.affiliationUniversity of Sheffielden
cg.contributor.affiliationAustralian National Universityen
cg.identifier.doihttps://doi.org/10.1093/jxb/erz083en
cg.issn1460-2431en
cg.issue10en
cg.journalJournal of Experimental Botanyen
cg.volume70en
dc.contributor.authorGiuliani, Ritaen
dc.contributor.authorKarki, Shantaen
dc.contributor.authorCovshoff, Sarahen
dc.contributor.authorLin, Hsiang-Chunen
dc.contributor.authorCoe, Robert A.en
dc.contributor.authorKoteyeva, Nuria K.en
dc.contributor.authorQuick, W. Paulen
dc.contributor.authorvon Caemmerer, Susanneen
dc.contributor.authorFurbank, Robert T.en
dc.contributor.authorHibberd, Julian M.en
dc.contributor.authorEdwards, Gerald E.en
dc.contributor.authorCousins, Asaph B.en
dc.date.accessioned2024-12-19T12:54:13Zen
dc.date.available2024-12-19T12:54:13Zen
dc.identifier.urihttps://hdl.handle.net/10568/164716
dc.titleKnockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leavesen
dcterms.abstractThe influence of reduced glycine decarboxylase complex (GDC) activity on leaf atmosphere CO2 and (CO2)-C-13 exchange was tested in transgenic Oryza sativa with the GDC H-subunit knocked down in leaf mesophyll cells. Leaf measurements on transgenic gdch knockdown and wild-type plants were carried out in the light under photorespiratory and low photorespiratory conditions (i.e. 18.4 kPa and 1.84 kPa atmospheric O-2 partial pressure, respectively), and in the dark. Under approximately current ambient O-2 partial pressure (18.4 kPa pO(2)), the gdch knockdown plants showed an expected photorespiratory-deficient phenotype, with lower leaf net CO2 assimilation rates (A) than the wild-type. Additionally, under these conditions, the gdch knockdown plants had greater leaf net discrimination against (CO2)-C-13 (Delta(o)) than the wild-type. This difference in Delta(o) was in part due to lower C-13 photorespiratory fractionation (f) ascribed to alternative decarboxylation of photorespiratory intermediates. Furthermore, the leaf dark respiration rate (R-d) was enhanced and the (CO2)-C-13 composition of respired CO2 (delta C-13(Rd)) showed a tendency to be more depleted in the gdch knockdown plants. These changes in R-d and delta C-13(Rd) were due to the amount and carbon isotopic composition of substrates available for dark respiration. These results demonstrate that impairment of the photorespiratory pathway affects leaf (CO2)-C-13 exchange, particularly the C-13 decarboxylation fractionation associated with photorespirationen
dcterms.accessRightsOpen Access
dcterms.available2019-03-06
dcterms.bibliographicCitationGiuliani, Rita; Karki, Shanta; Covshoff, Sarah; Lin, Hsiang-Chun; Coe, Robert A; Koteyeva, Nuria K; Quick, W Paul; Von Caemmerer, Susanne; Furbank, Robert T; Hibberd, Julian M; Edwards, Gerald E and Cousins, Asaph B. 2019. Knockdown of glycine decarboxylase complex alters photorespiratory carbon isotope fractionation in Oryza sativa leaves. Journal Of Experimental Botany, Volume 70, no. 10; pages 2773-2786.en
dcterms.extentpp. 2773-2786en
dcterms.issued2019-05-09
dcterms.languageen
dcterms.licenseCC-BY-NC-4.0
dcterms.publisherOxford University Pressen
dcterms.typeJournal Article

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