A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies

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cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169
cg.creator.identifierRichard Bishop: 0000-0002-3720-9970
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1017/s0031182099005934en
cg.isijournalISI Journalen
cg.issn0031-1820en
cg.issn1469-8161en
cg.issue6en
cg.journalParasitologyen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriVACCINESen
cg.volume120en
dc.contributor.authorSkilton, Robert A.en
dc.contributor.authorMusoke, A.J.en
dc.contributor.authorWells, C.W.en
dc.contributor.authorYagi, Y.en
dc.contributor.authorNene, Vishvanath M.en
dc.contributor.authorSpooner, P.R.en
dc.contributor.authorGachanja, J.en
dc.contributor.authorOsaso, J.en
dc.contributor.authorBishop, Richard P.en
dc.contributor.authorMorzaria, S.P.en
dc.date.accessioned2014-02-26T16:15:35Zen
dc.date.available2014-02-26T16:15:35Zen
dc.identifier.urihttps://hdl.handle.net/10568/35036
dc.titleA 32 kDa surface antigen of Theileria parva: Characterization and immunization studiesen
dcterms.abstractPrevious studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piroplasm surface antigen (mMPSA) of T. parva was cloned and expressed in bacteria. The expressed product reacted strongly with mAb 4C9, demonstrating identity between the p32 and mMPSA of T. parva. Using immunoblot analysis and immunoelectron microscopy with mAb 4C9 it was shown that the mMPSA is a major antigen of the merozoite and piroplasm at the cell surface, while lower levels of antigen are expressed in the sporozoite and schizont stages. Upregulation of the mMPSA occurs at merogony and can be induced by culturing schizont-infected lymphocytes at 42°C. Recombinant mMPSA of T. parva induced high titres of specific antibodies in cattle but failed to confer protection against a T. parva sporozoite stabilate challenge. The pre-challenge sera also failed to neutralize infectivity of sporozoites in an in vitro assay. Possible reasons for the lack of parasite neutralization in vivo and in vitro are discussed.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.available2000-06-01
dcterms.bibliographicCitationSkilton, R.A., Musoke, A.J., Wells, C.W., Yagi, Y., Nene, V., Spooner, P.R., Gachanja, J., Osaso, J., Bishop, R.P. and Morzaria, S.P. 2000. A 32 kDa surface antigen of Theileria parva: Characterization and immunization studies. Parasitology 120(6):553-564.en
dcterms.extentp. 553-564en
dcterms.issued2000-06
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherCambridge University Pressen
dcterms.subjectdisease controlen
dcterms.subjectanimal diseasesen
dcterms.typeJournal Article

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