Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions

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cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationAarhus Universityen
cg.contributor.crpGenebanks
cg.contributor.crpLivestock
cg.contributor.donorGlobal Crop Diversity Trusten
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeGenebanks
cg.coverage.countryEthiopia
cg.coverage.iso3166-alpha2ET
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierChris Jones: 0000-0001-9096-9728en
cg.creator.identifierAlice Muchugi: 0000-0002-8901-1376en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.15258/sst.2023.51.3.12en
cg.isijournalISI Journalen
cg.issn1819-5717en
cg.issue3en
cg.journalSeed Science and Technologyen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.ilriAGRICULTUREen
cg.subject.impactAreaEnvironmental health and biodiversity
cg.volume51en
dc.contributor.authorOlbana, Tamiruen
dc.contributor.authorMuchugi, Aliceen
dc.contributor.authorWoldemariam, Yeshien
dc.contributor.authorHay, Fiona R.en
dc.contributor.authorNdiwa, Nicholasen
dc.contributor.authorJones, Christopher S.en
dc.date.accessioned2023-12-12T08:03:47Zen
dc.date.available2023-12-12T08:03:47Zen
dc.identifier.urihttps://hdl.handle.net/10568/135256
dc.titleOvercoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessionsen
dcterms.abstractDesmanthus virgatus is a perennial forage legume used as a livestock feed source. Seeds of this species are physically dormant and thus, propagation is difficult. The dormancy also impacts the routine viability monitoring of genebank accessions. The present study investigated different methods to break dormancy in seeds collected from various locations at different times. The result showed that most pre-treatments significantly increased germination percentage. Accessions treated with concentrated sulphuric acid (25 or 30 minutes) followed by sandpaper scarification showed the highest proportion of normal seedlings (94.6 and 96%). Boiling water (2.3 or 3 minutes) was also effective in all accessions except for accession 331. However, accessions treated with hot water (3, 5 or 8 minutes) had a considerable proportion of hard seeds. The proportion of dead/abnormal seedlings was high in seeds treated with a hot wire in all accessions, ranging from 27.5 (accession 15489) to 75.5% (accession 16). The untreated seeds remained hard at the final germination count. We conclude that it is important to use concentrated sulphuric acid and mechanical scarification with sandpaper to break the dormancy for the species to enhance germination, obtain uniform field stands and make wise decisions during storage.en
dcterms.accessRightsOpen Access
dcterms.audienceCGIARen
dcterms.audienceDonorsen
dcterms.audienceScientistsen
dcterms.available2023-10-16en
dcterms.bibliographicCitationOlbana, T., Muchugi, A., Woldemariam, Y., Hay, F.R., Ndiwa, N. and Jones, C.S. 2023. Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions. Seed Science and Technology 51(3):421-434.en
dcterms.extentpp. 421-434en
dcterms.issued2023-10-16en
dcterms.languageen
dcterms.licenseCC-BY-NC-4.0
dcterms.publisherInternational Seed Testing Associationen
dcterms.subjectfabaceaeen
dcterms.subjectgenebanken
dcterms.subjecthedge lucerneen
dcterms.subjectphysical dormancyen
dcterms.subjectscarificationen
dcterms.subjectseed longevityen
dcterms.subjectviabilityen
dcterms.typeJournal Article

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ILRI feed and forage development program outputs
CGIAR Initiative on Genebanks
ILRI forage genebank:Team-wide outputs