Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions

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cg.authorship.typesCGIAR and advanced research instituteen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationAarhus Universityen_US
cg.contributor.crpGenebanksen_US
cg.contributor.crpLivestocken_US
cg.contributor.donorGlobal Crop Diversity Trusten_US
cg.contributor.donorCGIAR Trust Funden_US
cg.contributor.initiativeGenebanksen_US
cg.coverage.countryEthiopiaen_US
cg.coverage.iso3166-alpha2ETen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierChris Jones: 0000-0001-9096-9728en_US
cg.creator.identifierAlice Muchugi: 0000-0002-8901-1376en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.15258/sst.2023.51.3.12en_US
cg.isijournalISI Journalen_US
cg.issn1819-5717en_US
cg.issue3en_US
cg.journalSeed Science and Technologyen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.actionAreaGenetic Innovationen_US
cg.subject.ilriAGRICULTUREen_US
cg.subject.impactAreaEnvironmental health and biodiversityen_US
cg.volume51en_US
dc.contributor.authorOlbana, Tamiruen_US
dc.contributor.authorMuchugi, Aliceen_US
dc.contributor.authorWoldemariam, Yeshien_US
dc.contributor.authorHay, Fiona R.en_US
dc.contributor.authorNdiwa, Nicholasen_US
dc.contributor.authorJones, Christopher S.en_US
dc.date.accessioned2023-12-12T08:03:47Zen_US
dc.date.available2023-12-12T08:03:47Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/135256en_US
dc.titleOvercoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessionsen_US
dcterms.abstractDesmanthus virgatus is a perennial forage legume used as a livestock feed source. Seeds of this species are physically dormant and thus, propagation is difficult. The dormancy also impacts the routine viability monitoring of genebank accessions. The present study investigated different methods to break dormancy in seeds collected from various locations at different times. The result showed that most pre-treatments significantly increased germination percentage. Accessions treated with concentrated sulphuric acid (25 or 30 minutes) followed by sandpaper scarification showed the highest proportion of normal seedlings (94.6 and 96%). Boiling water (2.3 or 3 minutes) was also effective in all accessions except for accession 331. However, accessions treated with hot water (3, 5 or 8 minutes) had a considerable proportion of hard seeds. The proportion of dead/abnormal seedlings was high in seeds treated with a hot wire in all accessions, ranging from 27.5 (accession 15489) to 75.5% (accession 16). The untreated seeds remained hard at the final germination count. We conclude that it is important to use concentrated sulphuric acid and mechanical scarification with sandpaper to break the dormancy for the species to enhance germination, obtain uniform field stands and make wise decisions during storage.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceCGIARen_US
dcterms.audienceDonorsen_US
dcterms.audienceScientistsen_US
dcterms.available2023-10-16en_US
dcterms.bibliographicCitationOlbana, T., Muchugi, A., Woldemariam, Y., Hay, F.R., Ndiwa, N. and Jones, C.S. 2023. Overcoming dormancy in Desmanthus virgatu seeds for improved viability monitoring protocols of genebank accessions. Seed Science and Technology 51(3):421-434.en_US
dcterms.extentpp. 421-434en_US
dcterms.issued2023-10-16en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-NC-4.0en_US
dcterms.publisherInternational Seed Testing Associationen_US
dcterms.subjectfabaceaeen_US
dcterms.subjectgenebanken_US
dcterms.subjecthedge lucerneen_US
dcterms.subjectphysical dormancyen_US
dcterms.subjectscarificationen_US
dcterms.subjectseed longevityen_US
dcterms.subjectviabilityen_US
dcterms.typeJournal Articleen_US

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ILRI feed and forage development program outputs
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ILRI forage genebank:Team-wide outputs