Selection for resistance to cassava mosaic disease in African cassava germplasm using single nucleotide polymorphism markers

cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationUniversity of Ibadanen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorAfrican Unionen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeAccelerated Breeding
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.creator.identifierPaterne AGRE: 0000-0003-1231-2530
cg.creator.identifierIsmail Rabbi: 0000-0001-9966-2941
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.17159/sajs.2022/11607en
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journalen
cg.issn0038-2353en
cg.issue1-2: 11607en
cg.journalSouth African Journal of Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.iitaAGRONOMYen
cg.subject.iitaBIOSCIENCEen
cg.subject.iitaCASSAVAen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume118en
dc.contributor.authorCodjia, E.D.en
dc.contributor.authorOlasanmi, B.en
dc.contributor.authorAgre, A.P.en
dc.contributor.authorUwugiaren, R.en
dc.contributor.authorIge, A.D.en
dc.contributor.authorRabbi, I.Y.en
dc.date.accessioned2022-02-22T09:13:55Zen
dc.date.available2022-02-22T09:13:55Zen
dc.identifier.urihttps://hdl.handle.net/10568/118206
dc.titleSelection for resistance to cassava mosaic disease in African cassava germplasm using single nucleotide polymorphism markersen
dcterms.abstractCassava mosaic disease (CMD) is one of the main constraints that hamper cassava production. Breeding for varieties that are CMD resistant is a major aim in cassava breeding programmes. However, the use of the conventional approach has its limitations, including a lengthy growth cycle and a low multiplication rate of planting materials. To increase breeding efficiency as well as genetic gain of traits, SNP markers can be used to screen and identify resistant genotypes. The objective of this study was to predict the performance of 145 cassava genotypes from open-pollinated crosses for CMD resistance using molecular markers. Two SNP markers (S12_7926132 and S14_4626854), previously converted into Kompetitive allele-specific PCR (KASP) assays, as well as CMD incidence and severity scores, were used for selection. About 76% of the genotypes were revealed to be resistant to CMD based on phenotypic scores, while over 24% of the total population were found to be susceptible. Significant effects were observed for alleles associated with marker S12_7926132 while the other marker had non-significant effects. The predictive accuracy (true positives and true negatives) of the major CMD2 locus on chromosome 12 was 77% in the population used in this study. Our study provides insight into the potential use of marker-assisted selection for CMD resistance in cassava breeding programmes. Significance: With an aim towards reducing the food insecurity rate in Africa, we report on the use of genetic tools for a fast and efficient release of new cassava varieties to benefit breeders, farmers and consumers, given the food and industrial importance of this staple crop. This study adds tremendous knowledge to phenotypic and molecular screening for CMD resistance. The outcome will encourage breeders in various cassava breeding programmes to accelerate genetic gains as well as increase breeding accuracy and efficiency for CMD resistance.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2022-01-27
dcterms.bibliographicCitationCodjia, E.D., Olasanmi, B., Agre, A.P., Uwugiaren, R., Ige, A.D. & Rabbi, I.Y. (2022). Selection for resistance to cassava mosaic disease in African cassava germplasm using single nucleotide polymorphism markers. South African Journal of Science, 118(1/2): 11607, 1-7.en
dcterms.extent1-7en
dcterms.issued2022
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherAcademy of Science of South Africaen
dcterms.subjectcassavaen
dcterms.subjectafrican cassava mosaic virusen
dcterms.subjectsingle nucleotide polymorphismen
dcterms.subjectgenotypesen
dcterms.subjectmarker-assisted selectionen
dcterms.typeJournal Article

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