Diagnostic Tools Applied by ICARDA’s Germplasm Health Unit (GHU) for Detection of Pests and Diseases Transmitted via Legume and Cereal Seeds

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cg.contributor.affiliationInternational Center for Agricultural Research in the Dry Areasen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeGenebanks
cg.coverage.countryLebanon
cg.coverage.countryMorocco
cg.coverage.iso3166-alpha2LB
cg.coverage.iso3166-alpha2MA
cg.coverage.regionWestern Asia
cg.coverage.regionNorthern Africa
cg.creator.identifierSafaa Kumari: 0000-0002-4492-6257
cg.creator.identifierAbdulrahman Moukahel: 0000-0003-4297-771X
cg.identifier.doihttps://doi.org/10.22268/ajpp-41.2.197225en
cg.subject.actionAreaGenetic Innovation
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactAreaPoverty reduction, livelihoods and jobs
cg.subject.impactAreaGender equality, youth and social inclusion
cg.subject.impactAreaEnvironmental health and biodiversity
cg.subject.sdgSDG 1 - No povertyen
cg.subject.sdgSDG 4 - Quality educationen
cg.subject.sdgSDG 15 - Life on landen
cg.subject.sdgSDG 17 - Partnerships for the goalsen
dc.contributor.authorKumari, Safaaen
dc.contributor.authorMoukahel, Abdulrahmanen
dc.contributor.authorEl Miziani, Inaamen
dc.contributor.authorDarwish, Imanen
dc.date.accessioned2023-12-11T20:22:25Zen
dc.date.available2023-12-11T20:22:25Zen
dc.identifier.urihttps://hdl.handle.net/10568/135230
dc.titleDiagnostic Tools Applied by ICARDA’s Germplasm Health Unit (GHU) for Detection of Pests and Diseases Transmitted via Legume and Cereal Seedsen
dcterms.abstractSeeds of cereals (wheat, barley) and legumes (faba bean, lentil, chickpea, grasspea) are important exchange materials for farming, crop production, and research at national, regional, and international levels, but with the exchanges comes the danger of introducing new pests (fungi, bacteria, viruses, nematodes, and insects). ICARDA’s Germplasm Health Unit (GHU) is responsible for the monitoring, clearance, and documentation of safe germplasm movement at the Center. All incoming and outgoing genetic resources and breeding germplasm must pass through a strict quarantine monitoring system, including seed health testing. To confirm the health status of seed samples, the GHU conducts different types of tests based on the crops and the nature of the target pest. These tests can be classified into the following four distinct detection groups: direct inspection, incubation tests, immunoassays, and molecular tests. Ideally, seed health tests should be sensitive, specific, rapid, robust, low-cost, and simple to implement and interpret. Thus, ICARDA’s GHU applies different detection assays as part of the monitoring and management system, i.e. (i) visual inspection and floating test for stored insects, (ii) centrifuge washing, agar plate and freezing blotter tests for fungi, (iii) TBIA and ELISA for viruses, (iv) nematode extraction test for nematodes, and (v) agglutination test for bacteria. These tests permit rapid analysis of a large number of samples at a relatively low cost. ICARDA's GHU also developed and applied molecular tools such as high throughput, short lead times, and accurate assays. To fulfill legal and regulatory requirements for certified seed classes and allow for seed movement across international boundaries, ICARDA’s GHU processes annually over 50k samples tested for around 650k diagnostic reactions, for the purpose of short- and long-term conservation, distribution to a minimum of 90 countries and elimination of approximately 10-15% of pest-contaminated samples that could not be curated using phytosanitary treatments. The rejected samples were contaminated generally with fungi, nematodes, and viruses.en
dcterms.accessRightsOpen Access
dcterms.available2023-06-01
dcterms.bibliographicCitationKumari, S. G, A. Moukahel, I. El-Miziani and I. Darwish. 2023. Diagnostic tools applied by ICARDA’s Germplasm Health Unit (GHU) for detection of pests and diseases transmitted via legume and cereal seeds. Abstracts of papers presented in a regional workshop entitled “Advances in Plant Health Technologies in Healthy Plant Propagation Materials Production and Distribution and its role in Food Security”, Basra – Iraq, 15-16 May 2023. Arab Journal of Plant Protection, 41(2): 201-202. https://doi.org/10.22268/AJPP-41.2.197225en
dcterms.formatPDFen
dcterms.issued2023-06-01
dcterms.languagear
dcterms.languageen
dcterms.licenseCC-BY-NC-4.0
dcterms.publisherArab Society for Plant Protectionen
dcterms.subjectlegumesen
dcterms.subjectcerealsen
dcterms.subjectseed and plant healthen
dcterms.subjectdiagnostic toolsen
dcterms.subjectfood secuityen
dcterms.subjectseed-borne diseases/pestsen
dcterms.typeConference Paper

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