Studies on a Nigerian isolate of banana streak badnavirus: II. Effect of intraplant variation on virus accumulation and reliability of diagnosis by ELISA

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cg.authorship.typesCGIAR single centreen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.donorFood and Agriculture Organization of the United Nationsen
cg.contributor.donorWorld Banken
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.identifier.doihttps://doi.org/10.1111/j.1744-7348.1998.tb05202.xen
cg.isijournalISI Journalen
cg.issn0003-4746en
cg.issue2en
cg.journalAnnals of Applied Biologyen
cg.reviewStatusPeer Reviewen
cg.subject.iitaBANANAen
cg.subject.iitaPLANT DISEASESen
cg.subject.iitaPLANTAINen
cg.volume132en
dc.contributor.authorDahal, G.en
dc.contributor.authorPasberg-Gauhl, C.en
dc.contributor.authorGauhl, F.en
dc.contributor.authorThottappilly, G.en
dc.contributor.authorHughes, J.en
dc.date.accessioned2018-07-05T06:30:17Zen
dc.date.available2018-07-05T06:30:17Zen
dc.identifier.urihttps://hdl.handle.net/10568/95945
dc.titleStudies on a Nigerian isolate of banana streak badnavirus: II. Effect of intraplant variation on virus accumulation and reliability of diagnosis by ELISAen
dcterms.abstractMonitoring of banana streak badnavirus (BSV) antigens and symptoms in naturally BSV‐infected plantain and banana (Musa spp.) plants showed a great variation in symptom expression, distribution and relative concentration of BSV between and within plants. Expression and distribution of symptoms was erratic within individual leaves as well as between different leaves of the same plant. The concentration of BSV antigens detected by triple antibody sandwich enzyme linked immunosorbent assay (TAS‐ELISA) varied in different plant parts including leaf lamina, midrib and pseudostem, roots and young ‘cigar' leaf. The concentration of BSV antigens was high in symptomatic tissues but was low or below the limits of detection in most asymptomatic tissues. During ‘hot dry' seasons when symptoms were not fully expressed, the concentration of BSV antigens in leaf tissues declined drastically, often below the detection limit of TAS‐ELISA. These results suggested that for more reliable detection of BSV antigens by TAS‐ELISA, it is advisable to index plants using composite tissue samples comprising as many leaves as possible for each plant and collected during cool and/or rainy seasons when symptom expression is generally severe.en
dcterms.accessRightsLimited Access
dcterms.available2008-06-28
dcterms.bibliographicCitationDahal, G., Pasberg-Gauhl, C., Gauhl, F., Thottappilly, G. & Hughes, J. (1998). Studies on a Nigerian isolate of banana streak badnavirus: II. Effect of intraplant variation on virus accumulation and reliability of diagnosis by ELISA. Annals of Applied Biology, 132(2), 263-275.en
dcterms.extentpp. 263-275en
dcterms.issued1998-04
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherWileyen
dcterms.subjectbanana streak virusen
dcterms.subjectmusabadnavirusen
dcterms.subjectbadnavirusen
dcterms.subjectvirus distributionen
dcterms.typeJournal Article

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