Measuring the genetic diversity of Xanthomonas axonopodis pv. manihotis within different fields in Colombia

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cg.coverage.countryColombia
cg.coverage.iso3166-alpha2CO
cg.coverage.regionSouth America
cg.identifier.doihttps://doi.org/10.1094/phyto.2000.90.7.683en
cg.isijournalISI Journalen
cg.issn0031-949Xen
cg.issue7en
cg.journalPhytopathologyen
cg.reviewStatusPeer Reviewen
cg.subject.ciatBIODIVERSITYen
cg.subject.ciatCASSAVAen
cg.subject.ciatPESTS AND DISEASESen
cg.volume90en
dc.contributor.authorRestrepo, S.en
dc.contributor.authorVelez, C.M.en
dc.contributor.authorVerdier, Valerie M.en
dc.date.accessioned2014-10-02T08:32:48Zen
dc.date.available2014-10-02T08:32:48Zen
dc.identifier.urihttps://hdl.handle.net/10568/43844
dc.titleMeasuring the genetic diversity of Xanthomonas axonopodis pv. manihotis within different fields in Colombiaen
dcterms.abstractCassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis, is a widespread disease that affects cassava (Manihot esculenta). We collected 238 X. axonopodis pv. manihotis strains by intensively sampling single fields in four edaphoclimatic zones (ECZs) in Colombia. DNA polymorphism of different X. axonopodis pv. manihotis populations was assessed by restriction fragment length polymorphism (RFLP) analyses, repetitive sequence-based polymerase chain reaction (rep-PCR), and amplified fragment length polymorphism (AFLP) assays. Genetic diversity, phenetic relationships among strains, and the coefficient of genetic differentiation were determined. All strains were tested for aggressiveness on the susceptible cassava cv. MCOL 1522. Strains were also tested for virulence on cassava differentials adapted to the strains' respective ECZs. Our study showed that the Colombian X. axonopodis pv. manihotis population has a high degree of genetic diversity. The hierarchical analysis of diversity showed genotypic differentiation at all levels, among ECZs, among fields within ECZs, and among strains within fields planted to several cassava genotypes. New RFLP haplotypes were detected, leading to the characterization of a new pathotype. Dendrograms from AFLP were more robust than those from RFLP data. A close association between the strains' geographical origin and DNA polymorphism was obtained using RFLP and AFLP data. We suggest that the host played a role in causing pathogen differentiation.en
dcterms.accessRightsOpen Access
dcterms.extentpp. 683-690en
dcterms.issued2000-07
dcterms.languageen
dcterms.publisherScientific Societiesen
dcterms.subjectmanihot esculentaen
dcterms.subjectxanthomonas axonopodisen
dcterms.subjectgenetic variationen
dcterms.subjectgenetic markersen
dcterms.subjectpolymerase chain reactionen
dcterms.subjectvariación genéticaen
dcterms.subjectmarcadores genéticosen
dcterms.subjectreacción de cadenas de polimerasaen
dcterms.typeJournal Article

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