Identifying and analyzing the diversity of resistance gene analogs in Colombian Rubus genotypes

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cg.coverage.countryColombia
cg.coverage.iso3166-alpha2CO
cg.coverage.regionLatin America
cg.coverage.regionSouth America
cg.identifier.doihttps://doi.org/10.1094/pdis-05-14-0475-reen
cg.isijournalISI Journalen
cg.issn0191-2917en
cg.issue7en
cg.journalPlant Diseaseen
cg.reviewStatusPeer Reviewen
cg.subject.ciatPESTS AND DISEASESen
cg.volume99en
dc.contributor.authorAfanador Kafury, Lucíaen
dc.contributor.authorÁlvarez, Elizabethen
dc.contributor.authorMejía, Juan Fernandoen
dc.contributor.authorGonzález, A.en
dc.date.accessioned2015-08-19T18:19:29Zen
dc.date.available2015-08-19T18:19:29Zen
dc.identifier.urihttps://hdl.handle.net/10568/67950
dc.titleIdentifying and analyzing the diversity of resistance gene analogs in Colombian Rubus genotypesen
dcterms.abstractFive Andean blackberry Rubus genotypes, three resistant and two susceptible to anthracnose, were used to identify regions in the Rubus genome with homology to disease-resistance genes found in other plant species. Polymerase chain reaction amplification with 12 pairs of primers and fragment cloning yielded 520 clones, of which 151 showed inserts between 500 and 700 bp long. When sequenced, 47 clones showed homology with two types of resistance genes, non-Toll/interleukin-1 receptor (TIR) nucleotide binding site (NBS) leucine-rich repeat (LRR) and TIR-NBS-LRR, thereby confirming their designation as resistance gene analogs (RGAs). The number of RGAs detected per Rubus genotype ranged from 7 to 11, with the highest in a wild resistant and a cultivated susceptible genotype. Rubus RGAs were also homologous with several non-TIR- and TIR-type RGAs found in other members of the Rosaceae family (Rosa hybrid cultivar, Rosa roxburghii, Malus × domestica, M. prunifolia, M. baccata, M. floribunda, Pyrus communis, Prunus persica, P. kansuensis, P. avium, and Fragaria vesca). Three RGAs shared identity with two Rosaceae RGAs associated with the CRPM1 locus for powdery mildew resistance in R. roxburghii and the Rosa hybrid cultivar. This is the first report on RGAs present in the Andean blackberry in Colombia.en
dcterms.accessRightsOpen Access
dcterms.bibliographicCitationAfanador Kafury, Lucía; Álvarez, Elizabeth; Mejía, J.F.; González, A.. 2015. Identifying and analyzing the diversity of resistance gene analogs in Colombian Rubus genotypes . Plant Disease. 99(7): 994-1001.en
dcterms.extentp. 994-1001en
dcterms.issued2015-07
dcterms.languageen
dcterms.publisherScientific Societiesen
dcterms.subjectrubusen
dcterms.subjectdisease resistanceen
dcterms.subjectgenotypesen
dcterms.subjectplant diseasesen
dcterms.subjectpests of plantsen
dcterms.subjectdnaen
dcterms.subjectcolletotrichumen
dcterms.subjectresistencia a la enfermedaden
dcterms.subjectgenotiposen
dcterms.subjectenfermedades de las plantasen
dcterms.subjectplagas de plantasen
dcterms.subjectadnen
dcterms.typeJournal Article

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