CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationPennsylvania State Universityen
cg.contributor.affiliationSRM Universityen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationPacific Northwest National Laboratories, USAen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorNational Science Foundation, United Statesen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.coverage.countryKenya
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2KE
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.coverage.regionWestern Africa
cg.creator.identifierLeena Tripathi: 0000-0001-5723-4981
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1007/s11240-021-02210-3en
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.isijournalISI Journalen
cg.issn0167-6857en
cg.issue1en
cg.journalPlant Cell, Tissue and Organ Cultureen
cg.reviewStatusPeer Reviewen
cg.subject.iitaAGRONOMYen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT DISEASESen
cg.subject.iitaPLANT HEALTHen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.iitaVALUE CHAINSen
cg.subject.impactAreaNutrition, health and food security
cg.subject.sdgSDG 1 - No povertyen
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume150en
dc.contributor.authorTrauger, M.en
dc.contributor.authorHile, A.en
dc.contributor.authorSreenivas, K.en
dc.contributor.authorShouse, E.M.en
dc.contributor.authorBhatt, J.en
dc.contributor.authorLai, T.en
dc.contributor.authorMohandass, R.en
dc.contributor.authorTripathi, L.en
dc.contributor.authorOgden, A.J.en
dc.contributor.authorCurtis, W.R.en
dc.date.accessioned2022-06-20T15:26:06Zen
dc.date.available2022-06-20T15:26:06Zen
dc.identifier.urihttps://hdl.handle.net/10568/119873
dc.titleCO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactoren
dcterms.abstractIn vitro plant propagation systems such as temporary immersion bioreactors (TIBs) are valuable tools that enable production of disease-free plants with improved traits. However, TIB systems can be expensive, difficult to implement, and prone to contamination due to sugar rich propagation media. Using rapidly growing chicory root cultures to expedite design-build-test cycles, we report here an improved, low-cost version of a previously reported Hydrostatically-driven TIB (Hy-TIB) that facilitates economical use of gas mixtures. Bioreactor improvements include decreased material costs, expanded modes of operation, and a horizontal orientation of a plastic film plant growth chambers that increase propagule light exposure. To take advantage of these improvements, we describe here experiments that evaluate the impacts of elevated CO2 on propagation of cacao (Theobroma cacao) secondary embryos and nodal cultures of yam (Dioscorea spp.) during both phototrophic and photomixotrophic growth. Our experiments show that elevated CO2 during plant propagation significantly improved both cacao and yam propagule development and eliminated the need for supplemental sugars in tissue culture growth media. Thus, our improved Hy-TIB shows potential as a simple, low-cost, and scalable propagation platform with cost-effective gas composition control and reduced risk of contamination overgrowth. We provide detailed instructions for assembly of this Hy-TIB design and discuss the implications of its adoption in food-insecure regions of the world.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2022-04-18
dcterms.bibliographicCitationTrauger, M., Hile, A., Sreenivas, K., Shouse, E.M., Bhatt, J., Lai, T., ... & Curtis, W.R. (2022). CO2 supplementation eliminates sugar-rich media requirement for plant propagation using a simple inexpensive temporary immersion photobioreactor. Plant Cell, Tissue and Organ Culture, 1-15.en
dcterms.extentp. 57-71en
dcterms.issued2022-07
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.publisherSpringeren
dcterms.subjectbioreactorsen
dcterms.subjectplant propagationen
dcterms.subjecttheobroma cacaoen
dcterms.subjectdioscoreaen
dcterms.subjectfood securityen
dcterms.subjectvalue chainen
dcterms.typeJournal Article

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