Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationChinese Academy of Agricultural Sciencesen
cg.contributor.affiliationZhengzhou Universityen
cg.contributor.affiliationHainan Yazhou Bay Seed Laboratory, Sanyaen
cg.contributor.affiliationLiaocheng Universityen
cg.contributor.affiliationInternational Potato Centeren
cg.contributor.affiliationVali-e-Asr University of Rafsanjanen
cg.contributor.affiliationZagazig Universityen
cg.contributor.affiliationUniversity of Saskatchewanen
cg.contributor.donorNational Natural Science Foundation of Chinaen
cg.contributor.donorChinese Academy of Agricultural Sciencesen
cg.creator.identifierPhilip Kear: 0000-0002-4488-6013
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.jare.2021.10.014en
cg.isijournalISI Journalen
cg.issn2090-1224en
cg.journalJournal of Advanced Researchen
cg.reviewStatusPeer Reviewen
cg.subject.cipGENETIC RESOURCESen
cg.subject.cipGENETICS, GENOMICS AND CROP IMPROVEMENT SCIENCES GGCIen
cg.volume39en
dc.contributor.authorLi, L.en
dc.contributor.authorZhu, T.en
dc.contributor.authorSong, Y.en
dc.contributor.authorFeng, L.en
dc.contributor.authorKear, P.en
dc.contributor.authorRiseh, R.S.en
dc.contributor.authorSitohy, M.en
dc.contributor.authorDatla, R.en
dc.contributor.authorRen, M.en
dc.date.accessioned2022-07-26T20:53:52Zen
dc.date.available2022-07-26T20:53:52Zen
dc.identifier.urihttps://hdl.handle.net/10568/120301
dc.titleSalicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporumen
dcterms.abstractIntroduction: Biofungicides with low toxicity and high efficiency are a global priority for sustainable agricultural development. Phytohormone salicylic acid (SA) is an ancient medicine against various diseases in humans and activates the immune system in plants, but little is known of its function as a biofungicide. Objectives: Here, Fusarium oxysporum, the causal agent of devastating Fusarium wilt and immunodepressed patients, was used as a model system to explore whether SA can enter the pathogen cells and suppress key targets of the pathogen. Methods: Oxford Nanopore MinION sequencing and high-throughput chromosome conformation capture (Hi-C) sequencing were used to analyzed the genome of F. oxysporum. In addition, RNA-seq, qRT-PCR, and western blotting were conducted to detect gene and protein expression levels.Results: We isolated and sequenced the genome of F. oxysporum from potato dry rot, and the F. oxysporum included 12 chromosomes and 52.3 Mb genomic length. Pharmacological assays showed that exogenous application of SA can efficiently arrest hyphal growth, spore production, and pathogenicity of F. oxysporum, whereas endogenous salicylate hydroxylases significantly detoxify SA. The synergistic growth inhibition of F. oxysporum was observed when SA was combined with rapamycin. Kinase assays showed that SA inhibits FoTOR complex 1 (FoTORC1) by activating FoSNF1 in vivo. Transgenic potato plants with the interference of FoTOR1 and FoSAH1 genes inhibited the invasive growth of hyphae and significantly prevented the occurrence of Fusarium wilt. Conclusion: This study revealed the underlying mechanisms of SA against F. oxysporum and provided insights into SA in controlling various fungal diseases by targeting the SNF1-TORC1 pathway of pathogens.en
dcterms.accessRightsOpen Access
dcterms.audienceAcademicsen
dcterms.audienceCGIARen
dcterms.audienceDevelopment Practitionersen
dcterms.audienceDonorsen
dcterms.audienceExtensionen
dcterms.audienceFarmersen
dcterms.audienceGeneral Publicen
dcterms.audienceNGOsen
dcterms.audiencePolicy Makersen
dcterms.audienceScientistsen
dcterms.bibliographicCitationLi, L.; Zhu, T.; Song, Y.; Feng, L.; Kear, P. J.; Riseh, R. S.; Sitohy, M.; Datla, R.; Ren, M. 2022. Salicylic acid fights against Fusarium wilt by inhibiting target of rapamycin signaling pathway in Fusarium oxysporum. Journal of Advanced Research, 39. ISSN 2090-1224. 1–13.en
dcterms.extentp. 1-13en
dcterms.issued2022-07
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherElsevieren
dcterms.subjectsalicylic acidsen
dcterms.subjectpathogenicityen
dcterms.subjectfusarium oxysporumen
dcterms.typeJournal Article

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