Micropropagation of Launaea cornuta - an important indigenous vegetable and medicinal plant

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationUniversity of Eldoreten
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.crpGenebanks
cg.coverage.countryKenya
cg.coverage.countryMalawi
cg.coverage.countryMozambique
cg.coverage.countryTanzania
cg.coverage.countryUganda
cg.coverage.countryZimbabwe
cg.coverage.iso3166-alpha2KE
cg.coverage.iso3166-alpha2MW
cg.coverage.iso3166-alpha2MZ
cg.coverage.iso3166-alpha2TZ
cg.coverage.iso3166-alpha2UG
cg.coverage.iso3166-alpha2ZW
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.coverage.regionSouthern Africa
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.5897/ajb2016.15260en
cg.identifier.iitathemeBIOTECH & PLANT BREEDING
cg.identifier.urlhttps://www.ajol.info/index.php/ajb/article/view/142208en
cg.issn1684-5315en
cg.issue32en
cg.journalAfrican Journal of Biotechnologyen
cg.reviewStatusPeer Reviewen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaTISSUE CULTUREen
cg.volume15en
dc.contributor.authorAmbajo, F.en
dc.contributor.authorMatheka, J.M.en
dc.date.accessioned2016-09-09T12:21:09Zen
dc.date.available2016-09-09T12:21:09Zen
dc.identifier.urihttps://hdl.handle.net/10568/76989
dc.titleMicropropagation of Launaea cornuta - an important indigenous vegetable and medicinal planten
dcterms.abstractAn efficient micropropagation protocol was developed for the medicinal plant Launaea cornuta using green house axillary buds as explants. The best sterility was obtained at 30% (v/v) local bleach (JIK). Maximum shoot induction rate was achieved when axillary buds were cultured on Murashige and Skoog (MS) Media supplemented with 0.5 mg/L of 6-benzylaminopurine (BAP) for 3 weeks. The highest number of shoot multiplication was obtained when induced shoots were culture on MS media supplemented with 0.5 mg/L BAP and 0.2 mg/L NAA for 30 days. The best rooting response with regard to average root length, rooting percentage and number of roots was achieved within 4 weeks of culture of excised shoots on MS media having 0.5 mg/L BAP. Regenerated plants were successfully acclimatized and about 80 to 90% of plantlets survived under ex vitro conditions. About 170 plants were produced from a single nodal bud of L. cornuta after 60 days. A reproducible protocol was established for in vitro propagation of L. cornuta, an important indigenous vegetable with high medicinal value.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2016-08-10
dcterms.bibliographicCitationAmbajo, F. & Matheka, J.M. (2016). Micropropagation of Launaea cornuta-an important indigenous vegetable and medicinal plant. African Journal of Biotechnology, 15(32), 1726-1730.en
dcterms.extent1726-1730en
dcterms.issued2016-08-10
dcterms.languageen
dcterms.publisherAcademic Journalsen
dcterms.subjecttissue cultureen
dcterms.subjectmicropropagationen
dcterms.subjectmedicinal planten
dcterms.typeJournal Article

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