Comparative transcriptomics of the bovine apicomplexan parasite Theileria parva developmental stages reveals massive gene expression variation and potential vaccine antigens

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationPan African Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationUniversity of Edinburghen
cg.contributor.affiliationUniversity of Marylanden
cg.contributor.crpLivestocken
cg.contributor.donorAfrican Unionen
cg.contributor.donorDepartment of Foreign Affairs and Trade, Australiaen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.coverage.regionAfricaen
cg.creator.identifierJohn Juma: 0000-0002-1481-5337en
cg.creator.identifierChristian Keambou Tiambo: 0000-0002-7401-753Xen
cg.creator.identifierEunice Machuka: 0000-0002-1142-3442en
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen
cg.creator.identifierAppolinaire Djikeng: 0000-0001-9271-3419en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3389/fvets.2020.00287en
cg.isijournalISI Journalen
cg.issn2297-1769en
cg.journalFrontiers in Veterinary Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriCATTLEen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriECFen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriVACCINESen
cg.volume7en
dc.contributor.authorAtchou, K.en
dc.contributor.authorOngus, J.en
dc.contributor.authorMachuka, Eunice M.en
dc.contributor.authorJuma, Johnen
dc.contributor.authorTiambo, Christian K.en
dc.contributor.authorDjikeng, Appolinaireen
dc.contributor.authorSilva, Joana C.en
dc.contributor.authorPelle, Rogeren
dc.date.accessioned2020-06-28T18:44:27Zen
dc.date.available2020-06-28T18:44:27Zen
dc.identifier.urihttps://hdl.handle.net/10568/108622
dc.titleComparative transcriptomics of the bovine apicomplexan parasite Theileria parva developmental stages reveals massive gene expression variation and potential vaccine antigensen
dcterms.abstractTheileria parva is a protozoan parasite that causes East Coast fever (ECF), an economically important disease of cattle in Africa. It is transmitted mainly by the tick Rhipicephalus appendiculatus. Research efforts to develop a subunit vaccine based on parasite neutralizing antibodies and cytotoxic T-lymphocytes have met with limited success. The molecular mechanisms underlying T. parva life cycle stages in the tick vector and bovine host are poorly understood, thus limiting progress toward an effective and efficient control of ECF. Transcriptomics has been used to identify candidate vaccine antigens or markers associated with virulence and disease pathology. Therefore, characterization of gene expression throughout the parasite's life cycle should shed light on host-pathogen interactions in ECF and identify genes underlying differences in parasite stages as well as potential, novel therapeutic targets. Recently, the first gene expression profiling of T. parva was conducted for the sporoblast, sporozoite, and schizont stages. The sporozoite is infective to cattle, whereas the schizont is the major pathogenic form of the parasite. The schizont can differentiate into piroplasm, which is infective to the tick vector. The present study was designed to extend the T. parva gene expression profiling to the piroplasm stage with reference to the schizont. Pairwise comparison revealed that 3,279 of a possible 4,084 protein coding genes were differentially expressed, with 1,623 (49%) genes upregulated and 1,656 (51%) downregulated in the piroplasm relative to the schizont. In addition, over 200 genes were stage-specific. In general, there were more molecular functions, biological processes, subcellular localizations, and pathways significantly enriched in the piroplasm than in the schizont. Using known antigens as benchmarks, we identified several new potential vaccine antigens, including TP04_0076 and TP04_0640, which were highly immunogenic in naturally T. parva-infected cattle. All the candidate vaccine antigens identified have yet to be investigated for their capacity to induce protective immune response against ECF.en
dcterms.accessRightsOpen Accessen
dcterms.audienceScientistsen
dcterms.available2020-06-09en
dcterms.bibliographicCitationAtchou, K., Ongus, J., Machuka, E., Juma, J., Tiambo, C., Djikeng, A., Silva, J.C. and Pelle, R. 2020. Comparative transcriptomics of the bovine apicomplexan parasite Theileria parva developmental stages reveals massive gene expression variation and potential vaccine antigens. Frontiers in Veterinary Science 7:287.en
dcterms.issued2020-06-09en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherFrontiers Mediaen
dcterms.subjectanimal diseasesen
dcterms.subjecteast coast feveren
dcterms.subjectcattleen
dcterms.subjectdisease controlen
dcterms.subjectlivestocken
dcterms.subjectvaccinesen
dcterms.subjectparasitesen
dcterms.typeJournal Articleen

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