Sampling strategies for proper quantification of carotenoid content in cassava breeding

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cg.coverage.countryColombia
cg.coverage.iso3166-alpha2CO
cg.coverage.regionLatin America
cg.coverage.regionSouth America
cg.creator.identifierHernan Ceballos: 0000-0002-8744-7918
cg.identifier.urlhttp://www.academicjournals.org/journal/JPBCS/article-abstract/56504BD5026en
cg.isijournalISI Journalen
cg.issn2006-9758en
cg.journalJournal of Plant Breeding and Crop Scienceen
cg.reviewStatusPeer Reviewen
cg.subject.ciatCASSAVAen
cg.subject.ciatPLANT BREEDINGen
dc.contributor.authorOrtíz, D.en
dc.contributor.authorSánchez, T.en
dc.contributor.authorMorante, Nen
dc.contributor.authorCeballos, H.en
dc.contributor.authorPachón, Helenaen
dc.contributor.authorDuque E., Myriam Cristinaen
dc.contributor.authorChavez, Alba L.en
dc.contributor.authorEscobar Salamanca, Andrés F.en
dc.date.accessioned2014-10-02T08:33:11Zen
dc.date.available2014-10-02T08:33:11Zen
dc.identifier.urihttps://hdl.handle.net/10568/44068
dc.titleSampling strategies for proper quantification of carotenoid content in cassava breedingen
dcterms.abstractRapid-cycling recurrent selection has been implemented to produce cassava varieties that deliver provitamin A carotenoids to human populations that are affected by vitamin A deficiency. Rapid-cycling selection relies on quantification in just one plant per genotype. This study was conducted to assess the reliability of single-plant evaluations and the effect of age of the plant on carotenoid content in cassava roots. Variation in aliquot quantifications from the same root was negligible indicating a reliable experimental procedure. A large source of variation for carotenoids was due to differences among genotypes. Root-to-root variation from the same plant in some cases was surprisingly high and accounted for an average of 25% of the total variation. Plant-to-plant variation was not as high and accounted for 20% of the total variance. Carotenoid content was shown to vary depending on the age of the plant. Single-plant evaluations for carotenoid content in cassava, which is a requirement for rapidcycling recurrent selection is acceptable considering that it reduces in half the time required for evaluation and selection. However, it is suggested that 2 to 3 roots per plant be combined together in a sample to represent each genotype at a standard plant age.en
dcterms.accessRightsOpen Access
dcterms.issued2011
dcterms.languageen
dcterms.subjectmanihot esculentaen
dcterms.subjectgenetic gainen
dcterms.subjectcarotenoidsen
dcterms.subjectvarietiesen
dcterms.subjectvitamin deficienciesen
dcterms.subjectgenotypesen
dcterms.subjectmejora genéticaen
dcterms.subjectcarotenoidesen
dcterms.subjectvariedadesen
dcterms.subjectdeficiencia de vitaminasen
dcterms.subjectgenotiposen
dcterms.typeJournal Article

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