Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages

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cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationInstitute of Grassland and Environmental Research, United Kingdomen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationResearch Institute for Animal Production, Indonesiaen
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1017/s0263967x00032766en
cg.issn2053-5953en
cg.journalBSAP Occasional Publicationen
cg.number22en
cg.subject.ilriANIMAL FEEDINGen
cg.subject.ilriCROP-LIVESTOCKen
cg.subject.ilriFORAGESen
cg.volume22en
dc.contributor.authorDhanoa, M.S.en
dc.contributor.authorTanner, J.C.en
dc.contributor.authorOwen, E.en
dc.contributor.authorTheodorou, M.K.en
dc.contributor.authorWinugroho, H.M.en
dc.date.accessioned2018-12-14T17:05:15Zen
dc.date.available2018-12-14T17:05:15Zen
dc.identifier.urihttps://hdl.handle.net/10568/98590
dc.titleUse of an in vitro gas production method to study kinetics of rumen dry matter degradation of foragesen
dcterms.abstractIn addition to assessing rate and extent of gas production from fermenting forages in vitro with rumen micro-organisms, gas production methods (e.g. Theodorou et at, 1994) may be used also to study the degradation kinetics of forage dry matter and its fractions. As the substrate dry matter can be lost only through fermentation or solution, this removes the error inherent in the polyester bag method caused by fine particle losses from bags being deemed part of the ‘soluble’ fraction. The pressure transducer technique (PTT) of Theodorou et al. (1994) was used to measure gas production from nine tropical forage samples (Table 1). Nine bottles were prepared from each of the forage samples. Two bottles of each forage were harvested after 8, 24 and 48 h of incubation and a further three bottles at 72 h, to determine dry matter (DM) and organic matter (OM) losses. The same nine forages were assessed using the polyester bag method (Mehrez and Ørskov, 1977) to obtain DM and OM disappearance after rumen incubations of 4, 8, 24, 48 and 72 h. The soluble fraction was determined by hand washing. The reproducibility of measured losses, at given times, was examined using concordance (rc ) correlation (Lin, 1989) and mean square prediction error (MSPE, Bibby and Toutenberg, 1977). Also the simple exponential model was used to estimate the fractional rate of DM degradation (kd ) and asymptote A (%) for each forage and the values obtained using PTT (kd,ptt ) and in situ (kd,bag) compared using rc and MSPE.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.available2018-02-27
dcterms.bibliographicCitationDhanoa, M.S., Tanner, J.C., Owen, E., Theodorou, M.K. and Winugroho, H.M. 1998. Use of an in vitro gas production method to study kinetics of rumen dry matter degradation of forages. IN: In vitro techniques for measuring nutrient supply to ruminants. BSAP Occasional Publication 22: 249-251.en
dcterms.extentpp. 249-251en
dcterms.isPartOfBSAP Occasional Publicationen
dcterms.issued1998
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherCambridge University Pressen
dcterms.subjectanimal feedingen
dcterms.subjectforageen
dcterms.subjectmixed farmingen
dcterms.typeJournal Article

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