Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversité de Yaoundéen
cg.coverage.countryCameroon
cg.coverage.iso3166-alpha2CM
cg.coverage.regionAfrica
cg.coverage.regionMiddle Africa
cg.creator.identifierVincent Deblauwe: 0000-0001-9881-1052
cg.creator.identifierRachid Hanna: 0000-0002-5715-0144
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.21273/hortsci14556-19en
cg.identifier.iitathemeNATURAL RESOURCE MANAGEMENT
cg.isijournalISI Journalen
cg.issn0018-5345en
cg.issue4en
cg.journalHortScienceen
cg.reviewStatusPeer Reviewen
cg.subject.iitaAGRONOMYen
cg.subject.iitaFORESTRYen
cg.subject.iitaNATURAL RESOURCE MANAGEMENTen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.sdgSDG 1 - No povertyen
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume55en
dc.contributor.authorTchouga, A.O.en
dc.contributor.authorDeblauwe, V.en
dc.contributor.authorDjabou, S.A.M.en
dc.contributor.authorForgione, G.en
dc.contributor.authorHanna, R.en
dc.contributor.authorNiemenak, N.en
dc.date.accessioned2022-04-25T10:18:26Zen
dc.date.available2022-04-25T10:18:26Zen
dc.identifier.urihttps://hdl.handle.net/10568/119377
dc.titleMicropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiernen
dcterms.abstractThe jet-black to streaked ebony wood produced by the African ebony (Diospyros crassiflora Hiern, Ebenaceae) is exploited in Central and West Africa. A conservation effort is currently underway in Cameroon to propagate the tree through seedlings and cuttings. However, the intermittent availability of its seeds and the long time required for rooting formation of cuttings are limiting its propagation. This study aims to develop a successful protocol for ebony micropropagation. In vitro culture of nodal segments from seedlings was performed in half-strength Murashige and Skoog (MS) medium supplemented with either zeatin (0.0, 2.3, 4.6, 9.1, 13.7, 18.2, 22.8, and 27.4 µm) or 6-benzylaminopurine (BAP) (0.0, 2.2, 4.4, 8.8, 13.3, 17.8, 22.2, and 26.6 µm). After 12 weeks, all media allowed shoot budbreak. Shoots displaying the greatest budbreak were observed with 22.8 µm zeatin and 22.2 µm BAP. The shoots were then transferred to a medium supplemented with indole-3-butyric acid (IBA) and phloroglucinol (PG) at different concentrations for root induction. Root induction was observed on the shoots initially induced in the medium with BAP, but not in those grown in the medium with zeatin. In half-strength MS supplemented with 396.5 µm PG plus 14.2 µm IBA, the formation of a single tap root was observed on 79% of shoots, with an average root length of 2.8 ± 0.18 cm. Rooted plants were successfully acclimatized to the greenhouse, with a 50% survival rate. This is the first report on Diospyros crassiflora micropropagation, which paves the way for rapid ebony multiplication to respond to needed conservation efforts.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2020-03-05
dcterms.bibliographicCitationTchouga, A.O., Deblauwe, V., Djabou, S.A.M., Forgione, G., Hanna, R. & Niemenak, N. (2020). Micropropagation and effect of phloroglucinol on rooting of Diospyros crassiflora Hiern. HortScience, 55(4), 424-428.en
dcterms.extent424-428en
dcterms.issued2020-04
dcterms.languageen
dcterms.licenseCC-BY-NC-ND-4.0
dcterms.publisherAmerican Society for Horticultural Scienceen
dcterms.subjectin vitro cultureen
dcterms.subjectmicrocuttingsen
dcterms.subjectplant growth substancesen
dcterms.subjectmicropropagationen
dcterms.typeJournal Article

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