Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes

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cg.coverage.regionAfricaen
cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.ijpara.2009.07.004en
cg.issn0020-7519en
cg.issue1en
cg.journalInternational Journal for Parasitologyen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriANIMAL DISEASESen
cg.volume40en
dc.contributor.authorThekisoe, O.M.M.en
dc.contributor.authorRambritch, N.E.en
dc.contributor.authorNakao, R.en
dc.contributor.authorBazie, R.S.en
dc.contributor.authorMbati, P.en
dc.contributor.authorNamangala, B.en
dc.contributor.authorMalele, I.en
dc.contributor.authorSkilton, Robert A.en
dc.contributor.authorJongejan, F.en
dc.contributor.authorSugimoto, C.en
dc.contributor.authorKawazu, S.I.en
dc.contributor.authorInoue, N.en
dc.date.accessioned2009-12-30T09:00:14Zen
dc.date.available2009-12-30T09:00:14Zen
dc.identifier.urihttps://hdl.handle.net/10568/308
dc.titleLoop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genesen
dcterms.abstractWe have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1 fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries.en
dcterms.accessRightsLimited Accessen
dcterms.audienceScientistsen
dcterms.bibliographicCitationThekisoe, O.M.M.; Rambritch, N.E.; Nakao, R.; Bazie, R.S.; Mbati, P.; Namangala, B.; Malele, I.; Skilton, R.A.; Jongejan, F.; Sugimoto, C.; Kawazu, S.I.; Inoue, N. 2009. Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes. International Journal for Parasitology. v. 40(1). p. 55-61.en
dcterms.extentp. 55-61en
dcterms.issued2010-01en
dcterms.languageenen
dcterms.licenseCopyrighted; all rights reserveden
dcterms.publisherElsevieren
dcterms.subjecttheileria parvaen
dcterms.subjecteast coast feveren
dcterms.typeJournal Articleen

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