High-throughput field screening of cassava brown streak disease resistance for efficient and cost-saving breeding selection

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Culturesen
cg.contributor.affiliationInstitut National pour l’Étude et la Recherche Agronomiques, Democratic Republic of the Congoen
cg.contributor.crpRoots, Tubers and Bananas
cg.contributor.donorOne CGIAR Initiative Research Program on Roots, Tubers, and Bananasen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.initiativeAccelerated Breeding
cg.coverage.countryCongo, Democratic Republic of
cg.coverage.iso3166-alpha2CD
cg.coverage.regionAfrica
cg.coverage.regionWest and Central Africa
cg.creator.identifierMouritala Sikirou: 0000-0002-5164-6456en
cg.creator.identifierNajimu Adeniyi Adetoro: 0000-0003-1584-6394en
cg.creator.identifierSamar Sheat: 0000-0002-5649-7795en
cg.creator.identifierPierre-André Miafuntila: 0009-0006-4854-1166en
cg.creator.identifierFowobaje R. Kayode: 0000-0002-3995-160Xen
cg.creator.identifierIbnou Dieng: 0000-0002-1051-9143en
cg.creator.identifierZoumana BAMBA: 0000-0002-8913-3357en
cg.creator.identifierIsmail Rabbi: 0000-0001-9966-2941en
cg.creator.identifierHapson Mushoriwa: 0000-0001-8772-2409en
cg.creator.identifierStephan Winter: 0000-0003-0309-9934en
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.3390/agronomy15020425en
cg.identifier.iitathemeBIOTECH & PLANT BREEDINGen
cg.isijournalISI Journalen
cg.issn2073-4395en
cg.issue2: 425en
cg.journalAgronomyen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.iitaAGRONOMYen
cg.subject.iitaDISEASE CONTROLen
cg.subject.iitaCASSAVAen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT DISEASESen
cg.subject.iitaPLANT HEALTHen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.impactAreaNutrition, health and food security
cg.subject.impactPlatformNutrition, Health and Food Security
cg.subject.sdgSDG 2 - Zero hungeren
cg.volume25en
dc.contributor.authorSikirou, M.en
dc.contributor.authorAdetoro, N.A.en
dc.contributor.authorSheat, S.en
dc.contributor.authorMusungayi, E.en
dc.contributor.authorMusungangan, R.en
dc.contributor.authorPierre, M.en
dc.contributor.authorFowobaje, K.R.en
dc.contributor.authorDieng, I.en
dc.contributor.authorBamba, Z.en
dc.contributor.authorRabbi, I.Y.en
dc.contributor.authorMushoriwa, H.en
dc.contributor.authorWinter, S.en
dc.date.accessioned2025-03-18T08:21:21Zen
dc.date.available2025-03-18T08:21:21Zen
dc.identifier.urihttps://hdl.handle.net/10568/173680
dc.titleHigh-throughput field screening of cassava brown streak disease resistance for efficient and cost-saving breeding selectionen
dcterms.abstractCassava brown streak disease (CBSD) remains the most severe threat to cassava production in the Great Lakes region and Southern Africa. Screening for virus resistance by subjecting cassava to high virus pressure in the epidemic zone (hotspots) is a common but lengthy process because of unpredictable and erratic virus infections requiring multiple seasons for disease evaluation. This study investigated the feasibility of graft-infections to provide a highly controlled infection process that is robust and reproducible to select and eliminate susceptible cassava at the early stages and to predict the resistance of adapted and economically valuable varieties. To achieve this, a collection of cassava germplasm from the Democratic Republic of Congo and a different set of breeding trials comprising two seed nurseries and one preliminary yield trial were established. The cassava varieties OBAMA and NAROCASS 1 infected with CBSD were planted one month after establishment of the main trials in a 50 m2 plot to serve as the source of the infection and to provide scions to graft approximately 1 ha. Grafted plants were inspected for virus symptoms and additionally tested by RT-qPCR for sensitive detection of the viruses. The incidence and severity of CBSD and cassava mosaic disease (CMD) symptoms were scored at different stages of plant growth and fresh root yield determined at harvesting. The results from the field experiments proved that graft-infection with infected plants showed rapid symptom development in susceptible cassava plants allowing instant exclusion of those lines from the next breeding cycle. High heritability, with values ranging from 0.63 to 0.97, was further recorded for leaf and root symptoms, respectively. Indeed, only a few cassava progenies were selected while clones DSC260 and two species of M. glaziovii (Glaziovii20210005 and Glaziovii20210006) showed resistance to CBSD. Taken together, grafting scions from infected cassava is a highly efficient and cost-effective method to infect cassava with CBSD even under rugged field conditions. It replaces an erratic infection process with a controlled method to ensure precise screening and selection for virus resistance. The clones identified as resistant could serve as elite donors for introgression, facilitating the transfer of resistance to CBSD.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2025-02-08en
dcterms.bibliographicCitationSikirou, M., Adetoro, N.A., Sheat, S., Musungayi, E., Mungangan, R., Pierre, M., ... & Winter, S. (2025). High-throughput field screening of cassava brown streak disease resistance for efficient and cost-saving breeding selection. Agronomy, 15(2): 425, 1-15.en
dcterms.descriptionOpen Access Journalen
dcterms.extent1-15en
dcterms.issued2025en
dcterms.languageen
dcterms.licenseCC-BY-4.0
dcterms.subjectbreedingen
dcterms.subjectscreeningen
dcterms.subjectgraftingen
dcterms.subjectheritabilityen
dcterms.subjectcassavaen
dcterms.subjectfood securityen
dcterms.subjectplant diseasesen
dcterms.typeJournal Article

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