Comparison of field, greenhouse and detachedleaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.contributor.affiliationInternational Institute of Tropical Agricultureen_US
cg.contributor.affiliationUniversity of Ibadanen_US
cg.contributor.affiliationUniversity of Illinoisen_US
cg.contributor.affiliationNational Soybean Research Center, United Statesen_US
cg.coverage.countryNigeriaen_US
cg.coverage.countryUnited Statesen_US
cg.coverage.iso3166-alpha2NGen_US
cg.coverage.iso3166-alpha2USen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionACPen_US
cg.coverage.regionWestern Africaen_US
cg.coverage.regionNorthern Americaen_US
cg.identifier.doihttps://doi.org/10.1094/pdis-91-9-1161en_US
cg.isijournalISI Journalen_US
cg.issn0191-2917en_US
cg.issue9en_US
cg.journalPlant Diseaseen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.iitaNUTRITIONen_US
cg.subject.iitaSOYBEANen_US
cg.subject.iitaFOOD SECURITYen_US
cg.subject.iitaDISEASES CONTROLen_US
cg.subject.iitaGENETIC IMPROVEMENTen_US
cg.subject.iitaPESTS OF PLANTSen_US
cg.subject.iitaPLANT DISEASESen_US
cg.subject.iitaLIVELIHOODSen_US
cg.subject.iitaPLANT PRODUCTIONen_US
cg.subject.iitaHANDLING, TRANSPORT, STORAGE AND PROTECTION OF AGRICULTURAL PRODUCTSen_US
cg.subject.iitaPLANT BREEDINGen_US
cg.subject.iitaPLANT GENETIC RESOURCESen_US
cg.volume91en_US
dc.contributor.authorTwizeyimana, M.en_US
dc.contributor.authorOjiambo, P.S.en_US
dc.contributor.authorIkotun, T.en_US
dc.contributor.authorPaul, C.en_US
dc.contributor.authorHartman, G.L.en_US
dc.contributor.authorBandyopadhyay, Ranajiten_US
dc.date.accessioned2018-04-24T08:40:10Zen_US
dc.date.available2018-04-24T08:40:10Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/92181en_US
dc.titleComparison of field, greenhouse and detachedleaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizien_US
dcterms.abstractFourteen soybean accessions and breeding lines were evaluated for resistance to soybean rust caused by the fungus Phakopsora pachyrhizi. Evaluations were conducted in replicated experiments in growth chambers using detached leaves and under greenhouse and field conditions. In growth-chamber experiments, inoculation of detached leaves with 1 × 106 sporesml resulted in a significantly (P < 0.0001) higher total number of pustules and spores per unit leaf area than inoculations with lower spore concentrations. Amending agar medium with plant hormones significantly (P < 0.0001) aided retention of green leaf color in detached leaves. Leaf pieces on a medium containing kinetin at 10 mgliter had 5% chlorosis at 18 days after plating compared with leaf pieces on media amended with all other plant hormones, which had higher levels of chlorosis. Leaf age significantly affected number of pustules (P = 0.0146) and number of spores per pustule (P = 0.0088), and 3- to 4-week-old leaves had a higher number of pustules and number of spores per pustule compared with leaves that were either 1 to 2 or 5 to 6 weeks old. In detached-leaf and greenhouse screening, plants were evaluated for days to lesion appearance, days to pustule formation, days to pustule eruption, lesion number, lesion diameter, lesion type, number of pustules, and spores per pustule in 1-cm2 leaf area. Plants also were evaluated for diseased leaf area (in greenhouse and field screening) and sporulation (in field screening) at growth stage R6. There were significant (P < 0.0001) differences among genotypes in their response to P. pachyrhizi infection in the detached-leaf, greenhouse, and field evaluations. Accessions PI 594538A, PI 417089A, and UG-5 had very low levels of disease compared with the susceptible checks and all other genotypes. Detached-leaf, greenhouse, and field results were comparable, and there were significant correlations between detached-leaf and greenhouse (absolute r = 0.79; P < 0.0001) and between detached-leaf and field resistance (absolute r = 0.83; P < 0.0001) across genotypes. The overall results show the utility of detached-leaf assay for screening soybean for rust resistance.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.bibliographicCitationTwizeyimana, M., Ojiambo, P.S., Ikotun, T., Paul, C., Hartman, G.L. & Bandyopadhyay, R. (2007). Comparison of field, greenhouse, and detached-leaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi. Plant Disease, 91(9), 1161-1169.en_US
dcterms.extentpp. 1161-1169en_US
dcterms.issued2007-09en_US
dcterms.languageenen_US
dcterms.publisherScientific Societiesen_US
dcterms.subjectdisease resistanceen_US
dcterms.subjectsoybeansen_US
dcterms.subjectplant hormonesen_US
dcterms.subjectchlorosisen_US
dcterms.subjectspores per pustuleen_US
dcterms.subjectgenotypesen_US
dcterms.subjectphakopsora pachyrhizien_US
dcterms.subjectdetached-leafen_US
dcterms.typeJournal Articleen_US

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