Comparison of field, greenhouse and detachedleaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversity of Ibadanen
cg.contributor.affiliationUniversity of Illinoisen
cg.contributor.affiliationNational Soybean Research Center, United Statesen
cg.coverage.countryNigeria
cg.coverage.countryUnited States
cg.coverage.iso3166-alpha2NG
cg.coverage.iso3166-alpha2US
cg.coverage.regionAfrica
cg.coverage.regionACP
cg.coverage.regionWestern Africa
cg.coverage.regionNorthern America
cg.identifier.doihttps://doi.org/10.1094/pdis-91-9-1161en
cg.isijournalISI Journalen
cg.issn0191-2917en
cg.issue9en
cg.journalPlant Diseaseen
cg.reviewStatusPeer Reviewen
cg.subject.iitaNUTRITIONen
cg.subject.iitaSOYBEANen
cg.subject.iitaFOOD SECURITYen
cg.subject.iitaDISEASES CONTROLen
cg.subject.iitaGENETIC IMPROVEMENTen
cg.subject.iitaPESTS OF PLANTSen
cg.subject.iitaPLANT DISEASESen
cg.subject.iitaLIVELIHOODSen
cg.subject.iitaPLANT PRODUCTIONen
cg.subject.iitaHANDLING, TRANSPORT, STORAGE AND PROTECTION OF AGRICULTURAL PRODUCTSen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaPLANT GENETIC RESOURCESen
cg.volume91en
dc.contributor.authorTwizeyimana, M.en
dc.contributor.authorOjiambo, P.S.en
dc.contributor.authorIkotun, T.en
dc.contributor.authorPaul, C.en
dc.contributor.authorHartman, G.L.en
dc.contributor.authorBandyopadhyay, Ranajiten
dc.date.accessioned2018-04-24T08:40:10Zen
dc.date.available2018-04-24T08:40:10Zen
dc.identifier.urihttps://hdl.handle.net/10568/92181
dc.titleComparison of field, greenhouse and detachedleaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizien
dcterms.abstractFourteen soybean accessions and breeding lines were evaluated for resistance to soybean rust caused by the fungus Phakopsora pachyrhizi. Evaluations were conducted in replicated experiments in growth chambers using detached leaves and under greenhouse and field conditions. In growth-chamber experiments, inoculation of detached leaves with 1 × 106 sporesml resulted in a significantly (P < 0.0001) higher total number of pustules and spores per unit leaf area than inoculations with lower spore concentrations. Amending agar medium with plant hormones significantly (P < 0.0001) aided retention of green leaf color in detached leaves. Leaf pieces on a medium containing kinetin at 10 mgliter had 5% chlorosis at 18 days after plating compared with leaf pieces on media amended with all other plant hormones, which had higher levels of chlorosis. Leaf age significantly affected number of pustules (P = 0.0146) and number of spores per pustule (P = 0.0088), and 3- to 4-week-old leaves had a higher number of pustules and number of spores per pustule compared with leaves that were either 1 to 2 or 5 to 6 weeks old. In detached-leaf and greenhouse screening, plants were evaluated for days to lesion appearance, days to pustule formation, days to pustule eruption, lesion number, lesion diameter, lesion type, number of pustules, and spores per pustule in 1-cm2 leaf area. Plants also were evaluated for diseased leaf area (in greenhouse and field screening) and sporulation (in field screening) at growth stage R6. There were significant (P < 0.0001) differences among genotypes in their response to P. pachyrhizi infection in the detached-leaf, greenhouse, and field evaluations. Accessions PI 594538A, PI 417089A, and UG-5 had very low levels of disease compared with the susceptible checks and all other genotypes. Detached-leaf, greenhouse, and field results were comparable, and there were significant correlations between detached-leaf and greenhouse (absolute r = 0.79; P < 0.0001) and between detached-leaf and field resistance (absolute r = 0.83; P < 0.0001) across genotypes. The overall results show the utility of detached-leaf assay for screening soybean for rust resistance.en
dcterms.accessRightsLimited Access
dcterms.bibliographicCitationTwizeyimana, M., Ojiambo, P.S., Ikotun, T., Paul, C., Hartman, G.L. & Bandyopadhyay, R. (2007). Comparison of field, greenhouse, and detached-leaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi. Plant Disease, 91(9), 1161-1169.en
dcterms.extentpp. 1161-1169en
dcterms.issued2007-09
dcterms.languageen
dcterms.publisherScientific Societiesen
dcterms.subjectdisease resistanceen
dcterms.subjectsoybeansen
dcterms.subjectplant hormonesen
dcterms.subjectchlorosisen
dcterms.subjectspores per pustuleen
dcterms.subjectgenotypesen
dcterms.subjectphakopsora pachyrhizien
dcterms.subjectdetached-leafen
dcterms.typeJournal Article

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