Large-scale production and evaluation of marker-free indica rice IR64 expressing phytoferritin genes

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cg.identifier.doihttps://doi.org/10.1007/s11032-013-9931-zen_US
cg.issn1380-3743en_US
cg.issue1en_US
cg.journalMolecular Breedingen_US
cg.volume33en_US
dc.contributor.authorOliva, Normanen_US
dc.contributor.authorChadha-Mohanty, Prabhjiten_US
dc.contributor.authorPoletti, Susannaen_US
dc.contributor.authorAbrigo, Edithaen_US
dc.contributor.authorAtienza, Genelouen_US
dc.contributor.authorTorrizo, Linaen_US
dc.contributor.authorGarcia, Rubyen_US
dc.contributor.authorDueñas, Conradoen_US
dc.contributor.authorPoncio, Mar Aristeoen_US
dc.contributor.authorBalindong, Jeanetteen_US
dc.contributor.authorManzanilla, Marinaen_US
dc.contributor.authorMontecillo, Florenciaen_US
dc.contributor.authorZaidem, Maricrisen_US
dc.contributor.authorBarry, Gerarden_US
dc.contributor.authorHervé, Philippeen_US
dc.contributor.authorShou, Huxiaen_US
dc.contributor.authorSlamet-Loedin, Inez H.en_US
dc.date.accessioned2024-12-19T12:55:16Zen_US
dc.date.available2024-12-19T12:55:16Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/165611en_US
dc.titleLarge-scale production and evaluation of marker-free indica rice IR64 expressing phytoferritin genesen_US
dcterms.abstractBiofortification of rice (Oryza sativa L.) using a transgenic approach to increase the amount of iron in the grain is proposed as a low-cost, reliable, and sustainable solution to help developing countries combat anemia. In this study, we generated and evaluated a large number of rice or soybean ferritin over-accumulators in rice mega-variety IR64, including marker-free events, by introducing soybean or rice ferritin genes into the endosperm for product development. Accumulation of the protein was confirmed by ELISA, in situ immunological detection, and Western blotting. As much as a 37- and 19-fold increase in the expression of ferritin gene in single and co-transformed plants, respectively, and a 3.4-fold increase in Fe content in the grain over the IR64 wild type was achieved using this approach. Agronomic characteristics of a total of 1,860 progenies from 58 IR64 single independent transgenic events and 768 progenies from 27 marker-free transgenic events were evaluated and most trait characteristics did not show a penalty. Grain quality evaluation of high-Fe IR64 transgenic events showed quality similar to that of the wild-type IR64. To understand the effect of transgenes on iron homeostasis, transcript analysis was conducted on a subset of genes involved in iron uptake and loading. Gene expression of the exogenous ferritin gene in grain correlates with protein accumulation and iron concentration. The expression of NAS2 and NAS3 metal transporters increased during the grain milky stage.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.available2013-08-11en_US
dcterms.bibliographicCitationOliva, Norman; Chadha-Mohanty, Prabhjit; Poletti, Susanna; Abrigo, Editha; Atienza, Genelou; Torrizo, Lina; Garcia, Ruby; Dueñas, Conrado; Poncio, Mar Aristeo; Balindong, Jeanette; Manzanilla, Marina; Montecillo, Florencia; Zaidem, Maricris; Barry, Gerard; Hervé, Philippe; Shou, Huxia and Slamet-Loedin, Inez H. 2013. Large-scale production and evaluation of marker-free indica rice IR64 expressing phytoferritin genes. Mol Breeding, Volume 33 no. 1 p. 23-37en_US
dcterms.extentpp. 23-37en_US
dcterms.issued2014-01en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-2.0en_US
dcterms.publisherSpringeren_US
dcterms.subjectelisaen_US
dcterms.subjectferritinen_US
dcterms.subjecthomeostasisen_US
dcterms.subjectindica riceen_US
dcterms.subjectir64 (variety) productionen_US
dcterms.subjecttransgenic riceen_US
dcterms.subjectvarietiesen_US
dcterms.subjectwestern blottingen_US
dcterms.typeJournal Articleen_US

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