Application of fast technology for analysis (FTA) for sampling and recovery of deoxyribonucleic acid (DNA) for molecular characterization of cowpea breeding lines for Striga resistance

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.contributor.affiliationUniversity of Agriculture, Makurdien_US
cg.contributor.affiliationAhmadu Bello Universityen_US
cg.contributor.affiliationInternational Institute of Tropical Agricultureen_US
cg.contributor.affiliationUniversity of Virginiaen_US
cg.coverage.countryNigeriaen_US
cg.coverage.iso3166-alpha2NGen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionWestern Africaen_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.5897/ajb11.419en_US
cg.issn1684-5315en_US
cg.issue85en_US
cg.journalAfrican Journal of Biotechnologyen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.iitaCOWPEAen_US
cg.subject.iitaPESTS OF PLANTSen_US
cg.subject.iitaPLANT BREEDINGen_US
cg.subject.iitaAGRONOMYen_US
cg.volume10en_US
dc.contributor.authorOmoigui, L.O.en_US
dc.contributor.authorIshiyaku, M.F.en_US
dc.contributor.authorOusmane, B.en_US
dc.contributor.authorGowda, B.S.en_US
dc.contributor.authorTimko, M.P.en_US
dc.date.accessioned2017-11-14T08:07:47Zen_US
dc.date.available2017-11-14T08:07:47Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/89361en_US
dc.titleApplication of fast technology for analysis (FTA) for sampling and recovery of deoxyribonucleic acid (DNA) for molecular characterization of cowpea breeding lines for Striga resistanceen_US
dcterms.abstractStriga gesnerioides (Willd) Vatke is a significant constraint to cowpea production in the dry savannas of sub-Saharan Africa. Yield losses caused by S. gesnerioides in these regions are estimated in millions of tons annually and the prevalence of Striga soil infestation is steadily increasing. Conventional breeding efforts have developed some cowpea lines with Striga resistance as well as other important agronomic traits but it is time-consuming and difficult to pyramid favorable traits. The use of marker-assisted selection (MAS) makes it easier to select plant traits and reduce the time needed to develop new varieties. The potential of Fast Technology for Analysis (FTA) as an effective technology for sampling and retrieval of DNA from plant tissue and their subsequent molecular analysis was assessed in the laboratory. DNA was successfully recovered from the leaf tissues of cowpea pressed into the FTA® Classic card and the DNA obtained from the FTA papers was found to be suitable for molecular analysis by PCR-based techniques. The marker efficiency of Sequence Characterized Amplified Region (SCAR) marker MahSe2 and C42B was 93% in detecting SG3 resistance. This study demonstrated that the application of MAS using FTA technology has the potential to put the breeding process on a fast track and increase the efficiency of breeding activities.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2011-12-28en_US
dcterms.bibliographicCitationOmoigui, L.O., Ishiyaku, M.F., Ousmane, B., Gowda, B.S. & Timko, M.P. (2011). Application of fast technology for analysis (FTA)® for sampling and recovery of deoxyribonucleic acid (DNA) for molecular characterization of cowpea breeding lines for Striga resistance. African Journal of Biotechnology, 10(85), 19681-19986.en_US
dcterms.descriptionArticle purchaseden_US
dcterms.extent19681-19986en_US
dcterms.issued2011en_US
dcterms.languageenen_US
dcterms.publisherAcademic Journalsen_US
dcterms.subjectstriga gesnerioidesen_US
dcterms.subjecttissueen_US
dcterms.subjectcowpeasen_US
dcterms.subjectdnaen_US
dcterms.subjectyield lossesen_US
dcterms.subjectstriga resistanceen_US
dcterms.subjectmarker-assisted selectionen_US
dcterms.typeJournal Articleen_US

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