Application of fast technology for analysis (FTA) for sampling and recovery of deoxyribonucleic acid (DNA) for molecular characterization of cowpea breeding lines for Striga resistance

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationUniversity of Agriculture, Makurdien
cg.contributor.affiliationAhmadu Bello Universityen
cg.contributor.affiliationInternational Institute of Tropical Agricultureen
cg.contributor.affiliationUniversity of Virginiaen
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.5897/ajb11.419en
cg.issn1684-5315en
cg.issue85en
cg.journalAfrican Journal of Biotechnologyen
cg.reviewStatusPeer Reviewen
cg.subject.iitaCOWPEAen
cg.subject.iitaPESTS OF PLANTSen
cg.subject.iitaPLANT BREEDINGen
cg.subject.iitaAGRONOMYen
cg.volume10en
dc.contributor.authorOmoigui, L.O.en
dc.contributor.authorIshiyaku, M.F.en
dc.contributor.authorOusmane, B.en
dc.contributor.authorGowda, B.S.en
dc.contributor.authorTimko, M.P.en
dc.date.accessioned2017-11-14T08:07:47Zen
dc.date.available2017-11-14T08:07:47Zen
dc.identifier.urihttps://hdl.handle.net/10568/89361
dc.titleApplication of fast technology for analysis (FTA) for sampling and recovery of deoxyribonucleic acid (DNA) for molecular characterization of cowpea breeding lines for Striga resistanceen
dcterms.abstractStriga gesnerioides (Willd) Vatke is a significant constraint to cowpea production in the dry savannas of sub-Saharan Africa. Yield losses caused by S. gesnerioides in these regions are estimated in millions of tons annually and the prevalence of Striga soil infestation is steadily increasing. Conventional breeding efforts have developed some cowpea lines with Striga resistance as well as other important agronomic traits but it is time-consuming and difficult to pyramid favorable traits. The use of marker-assisted selection (MAS) makes it easier to select plant traits and reduce the time needed to develop new varieties. The potential of Fast Technology for Analysis (FTA) as an effective technology for sampling and retrieval of DNA from plant tissue and their subsequent molecular analysis was assessed in the laboratory. DNA was successfully recovered from the leaf tissues of cowpea pressed into the FTA® Classic card and the DNA obtained from the FTA papers was found to be suitable for molecular analysis by PCR-based techniques. The marker efficiency of Sequence Characterized Amplified Region (SCAR) marker MahSe2 and C42B was 93% in detecting SG3 resistance. This study demonstrated that the application of MAS using FTA technology has the potential to put the breeding process on a fast track and increase the efficiency of breeding activities.en
dcterms.accessRightsOpen Access
dcterms.audienceScientistsen
dcterms.available2011-12-28
dcterms.bibliographicCitationOmoigui, L.O., Ishiyaku, M.F., Ousmane, B., Gowda, B.S. & Timko, M.P. (2011). Application of fast technology for analysis (FTA)® for sampling and recovery of deoxyribonucleic acid (DNA) for molecular characterization of cowpea breeding lines for Striga resistance. African Journal of Biotechnology, 10(85), 19681-19986.en
dcterms.descriptionArticle purchaseden
dcterms.extent19681-19986en
dcterms.issued2011
dcterms.languageen
dcterms.publisherAcademic Journalsen
dcterms.subjectstriga gesnerioidesen
dcterms.subjecttissueen
dcterms.subjectcowpeasen
dcterms.subjectdnaen
dcterms.subjectyield lossesen
dcterms.subjectstriga resistanceen
dcterms.subjectmarker-assisted selectionen
dcterms.typeJournal Article

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