Evaluation of genetic variability in four Nigerian locally-adapted chicken populations using major histocompatibility complex-linked LEI0258 microsatellite marker

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationBowen Universityen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationObafemi Awolowo Universityen
cg.contributor.affiliationFederal University of Agriculture, Nigeriaen
cg.contributor.donorDepartment for Foreign Affairs and Trade, Australiaen
cg.contributor.donorSyngenta Foundation for Sustainable Agricultureen
cg.contributor.donorBill & Melinda Gates Foundationen
cg.contributor.donorDepartment for International Development, United Kingdomen
cg.contributor.donorSwedish International Development Cooperation Agencyen
cg.coverage.countryNigeria
cg.coverage.iso3166-alpha2NG
cg.coverage.regionAfrica
cg.coverage.regionWestern Africa
cg.creator.identifiermartina kyallo: 0000-0003-2837-5325en
cg.creator.identifierJean-Baka Domelevo Entfellner: 0000-0002-8282-1325en
cg.creator.identifierMartina kyalo: 0000-0001-9426-8152en
cg.creator.identifierRoger Pelle: 0000-0003-1053-085Xen
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.22069/psj.2023.20536.1854en
cg.isijournalISI Journalen
cg.issn2345-6566en
cg.issue2en
cg.journalPoultry Science Journalen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL BREEDINGen
cg.subject.ilriBREEDSen
cg.subject.ilriCHICKENSen
cg.subject.ilriGENETICSen
cg.subject.ilriGENETIC RESOURCESen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriPOULTRYen
cg.volume11en
dc.contributor.authorOladejo, O.A.en
dc.contributor.authorOseni, S.O.en
dc.contributor.authorKyallo, Martina M.en
dc.contributor.authorDomelevo Entfellner, Jean-Bakaen
dc.contributor.authorTor, N.E.en
dc.contributor.authorTiambo, Christian K.en
dc.contributor.authorPelle, Rogeren
dc.date.accessioned2023-10-02T12:39:25Zen
dc.date.available2023-10-02T12:39:25Zen
dc.identifier.urihttps://hdl.handle.net/10568/132092
dc.titleEvaluation of genetic variability in four Nigerian locally-adapted chicken populations using major histocompatibility complex-linked LEI0258 microsatellite markeren
dcterms.abstractMajor Histocompatibility Complex (MHC) is a group of genes that generally influence immune response in vertebrates, and it has been explored among different animal species in various countries. However, there is a paucity of information on its application in Nigerian locally-adapted chickens (NLAC). This research investigated genetic polymorphism, allele variability, and genetic relationships using LEI0258 major histocompatibility complex-linked microsatellite marker among four NLAC populations: Fulani × Yoruba ecotypes, FUNNAB Alpha × Noiler breeds. Blood samples were randomly collected from 50 mature birds in each population and DNA was extracted and subsequently subjected to PCR, Sanger sequencing, and bioinformatic analysis. There were two variable numbers of tandem repeats (VNTRs), with 90% of the alleles containing only one R13 and varying numbers of the R12 motifs that ranged from 1 to 19. Additional polymorphism was revealed by the presence of five SNPs and three indels in the upstream and downstream regions of LEI0258. A total of 48 alleles were observed with sizes ranging from 188 to 530 base pairs while the allele frequencies within the populations ranged from 1.9 to 29.2%. However, only 17 out of the 48 alleles had corresponding MHC-B haplotypes. Haplotypes B2, B12, and B21 found in this study had been reported to confer resistance to infectious poultry diseases especially avian influenza in locally adapted chickens. There were high allelic variability and genetic polymorphisms observed via the atypical LEI0258 microsatellite in describing the MHC-B region.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationOladejo, O.A., Oseni, S.O., Kyallo, M., Domelevo Entfellner, J.-B., Tor, N.E., Tiambo, C.K. and Pelle, R. 2023. Evaluation of genetic variability in four Nigerian locally-adapted chicken populations using major histocompatibility complex-linked LEI0258 microsatellite marker. Poultry Science Journal 11(2): 189-201.en
dcterms.extent189-201en
dcterms.issued2023-09-15en
dcterms.languageen
dcterms.licenseOther
dcterms.subjectlivestocken
dcterms.subjectgeneticsen
dcterms.subjectanimal genetic resourcesen
dcterms.subjectimmune responseen
dcterms.subjectchickensen
dcterms.typeJournal Article

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