Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze

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cg.authorship.typesNot CGIAR developing country instituteen_US
cg.contributor.affiliationHokkaido Universityen_US
cg.contributor.affiliationHokkaido Institute of Public Healthen_US
cg.contributor.affiliationYamaguchi Universityen_US
cg.contributor.affiliationNational Institute of Infectious Diseases, Japanen_US
cg.creator.identifierNaftaly Githaka: 0000-0003-4530-7164en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1016/j.ttbdis.2015.09.006en_US
cg.issn1877-959Xen_US
cg.issue1en_US
cg.journalTicks and Tick-borne Diseasesen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCATTLEen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriENVIRONMENTen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriPESTSen_US
cg.subject.ilriVACCINESen_US
cg.volume7en_US
dc.contributor.authorToyomane, K.en_US
dc.contributor.authorKonnai, S.en_US
dc.contributor.authorNiwa, A.en_US
dc.contributor.authorGithaka, Naftaly W.en_US
dc.contributor.authorIsezaki, M.en_US
dc.contributor.authorYamada, S.en_US
dc.contributor.authorIto, T.en_US
dc.contributor.authorTakano, A.en_US
dc.contributor.authorAndo, S.en_US
dc.contributor.authorKawabata, H.en_US
dc.contributor.authorMurata, S.en_US
dc.contributor.authorOhashi, K.en_US
dc.date.accessioned2017-04-10T07:47:07Zen_US
dc.date.available2017-04-10T07:47:07Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/80690en_US
dc.titleIdentification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulzeen_US
dcterms.abstractIxodes ricinus immunosuppressor (Iris) is a tick salivary gland protein derived from I. ricinus. In this study, Iris homolog was identified in the salivary glands of Ixodes persulcatus, which is the specific vector of the Lyme disease agent in Japan. The homolog was named Ipis-1. To investigate the function of Ipis-1, we prepared a recombinant Ipis-1 expressed in COS-7 cells as a rabbit IgG Fc-fused protein (Ipis-1-Ig). Cell proliferation assay and IFN-γ ELISA showed that Ipis-1-Ig inhibits the proliferation and IFN-γ production of bovine peripheral blood mononuclear cells (PBMCs). Notably, Ipis-1-Ig inhibited the cell proliferation and production of IFN-γ in bovine PBMCs even when CD14+ cells were depleted, suggesting that Ipis could directly interact with T cells and inhibit their functions. In conclusion, Ipis could contribute to the establishment of environments suitable for tick blood feeding and pathogen transmission by suppressing the function of immune cells.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationToyomane, K., Konnai, S., Niwa, A., Githaka, N., Isezaki, M., Yamada, S., Ito, T., Takano, A., Ando, S., Kawabata, H., Murata, S. and Ohashi, K. 2016. Identification and the preliminary in vitro characterization of IRIS homologue from salivary glands of Ixodes persulcatus Schulze. Ticks and Tick-borne Diseases 7(1): 119–125.en_US
dcterms.extentp. 119-125en_US
dcterms.issued2016-02en_US
dcterms.languageenen_US
dcterms.licenseCopyrighted; all rights reserveden_US
dcterms.publisherElsevieren_US
dcterms.subjectenvironmenten_US
dcterms.subjectsalivary glandsen_US
dcterms.subjectpathogensen_US
dcterms.subjectanimal diseasesen_US
dcterms.typeJournal Articleen_US

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