Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validation

Simple item page
cg.authorship.typesCGIAR and developing country instituteen
cg.authorship.typesCGIAR and advanced research instituteen
cg.contributor.affiliationCommonwealth Scientific and Industrial Research Organisation, Australiaen
cg.contributor.affiliationAgriculture Victoria, Australiaen
cg.contributor.affiliationInternational Maize and Wheat Improvement Centeren
cg.contributor.affiliationUniversity of Sydneyen
cg.contributor.affiliationInstituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Mexicoen
cg.contributor.donorGrains Research and Development Corporation, Australiaen
cg.contributor.donorCGIAR Trust Funden
cg.contributor.initiativeAccelerated Breeding
cg.creator.identifierBHOJA BASNET: 0000-0002-1693-4807
cg.creator.identifierMandeep Randhawa: 0000-0002-7410-6233
cg.creator.identifierJULIO HUERTA-ESPINO: 0000-0001-8334-9862
cg.creator.identifierRavi Prakash Singh: 0000-0002-4676-5071
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1007/s00122-022-04052-9en
cg.isijournalISI Journalen
cg.issn1432-2242en
cg.issue5en
cg.journalTheoretical and Applied Geneticsen
cg.reviewStatusPeer Reviewen
cg.subject.actionAreaGenetic Innovation
cg.subject.impactAreaNutrition, health and food security
cg.volume135en
dc.contributor.authorMago, Rohiten
dc.contributor.authorChunhong Chenen
dc.contributor.authorXiaodi Xiaen
dc.contributor.authorWhan, Alexen
dc.contributor.authorForrest, Kerrie L.en
dc.contributor.authorBasnet, Bhoja Rajen
dc.contributor.authorPerera, Geethaen
dc.contributor.authorChandramohan, Suthaen
dc.contributor.authorRandhawa, Mandeep S.en
dc.contributor.authorHayden, Matthewen
dc.contributor.authorBansal, Urmilen
dc.contributor.authorHuerta Espino, Julioen
dc.contributor.authorSingh, Ravi P.en
dc.contributor.authorBariana, Harbans S.en
dc.contributor.authorLagudah, Evans S.en
dc.date.accessioned2023-01-05T08:33:10Zen
dc.date.available2023-01-05T08:33:10Zen
dc.identifier.urihttps://hdl.handle.net/10568/126590
dc.titleAdult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker development and validationen
dcterms.abstractAn F3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F6 recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F6:7 population was developed through single seed descent method. F7 and F8 RILs were tested along with the parents at different locations. The F6 individual plants and both parents were genotyped using the 90 K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B (QSrGH.cs-1BL) and 2A (QSrGH.cs-2AL) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL. Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63. SNP markers associated with Sr63 were converted to kompetetive allele-specific PCR (KASP) assays and were validated on a set of durum cultivars.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.available2022-02-23
dcterms.bibliographicCitationMago, R., Chen, C., Xia, X., Whan, A., Forrest, K., Basnet, B.R., Perera, G., Chandramohan, S., Randhawa, M., Hayden, M., Bansal, U., Huerta-Espino, J., Singh, R.P., Bariana, H. and Lagudah, E. 2022. Adult plant stem rust resistance in durum wheat Glossy Huguenot: Mapping, marker develop-ment and validation. Theoretical and Applied Genetics 135(5):1541–1550.en
dcterms.extentp. 1541-1550en
dcterms.issued2022-05
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherSpringeren
dcterms.subjectgenesen
dcterms.subjectplantsen
dcterms.subjecthard wheaten
dcterms.subjectseedsen
dcterms.subjectrustsen
dcterms.subjectdisease resistanceen
dcterms.subjectallelesen
dcterms.typeJournal Article

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CGIAR Initiative on Accelerated Breeding
CIMMYT Journal Articles