Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

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cg.contributor.affiliationUniversity of Nairobien_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.crpAgriculture for Nutrition and Healthen_US
cg.coverage.countryKenyaen_US
cg.coverage.iso3166-alpha2KEen_US
cg.coverage.regionAfricaen_US
cg.coverage.regionEastern Africaen_US
cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1007/s11250-012-0202-5en_US
cg.isijournalISI Journalen_US
cg.issn0049-4747en_US
cg.issn1573-7438en_US
cg.issue1en_US
cg.journalTropical Animal Health and Productionen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriAGRI-HEALTHen_US
cg.subject.ilriDAIRYINGen_US
cg.subject.ilriEMERGING DISEASESen_US
cg.subject.ilriFARMING SYSTEMSen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriZOONOTIC DISEASESen_US
cg.volume44en_US
dc.contributor.authorKang'ethe, Erastus K.en_US
dc.contributor.authorMulinge, E.K.en_US
dc.contributor.authorSkilton, Robert A.en_US
dc.contributor.authorNjahira, Moses N.en_US
dc.contributor.authorMonda, J.G.en_US
dc.contributor.authorNyongesa, C.en_US
dc.contributor.authorMbae, C.K.en_US
dc.contributor.authorKamwati, S.K.en_US
dc.date.accessioned2012-09-29T06:02:37Zen_US
dc.date.available2012-09-29T06:02:37Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/24412en_US
dc.titleCryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenyaen_US
dcterms.abstractA total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twenty-five samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.audienceScientistsen_US
dcterms.available2012-07-15en_US
dcterms.bibliographicCitationKang’ethe, E.K., Mulinge, E.K., Skilton, R.A., Njahira, M., Monda, J.G., Nyongesa, C., Mbae, C.K. and Kamwati, S.K. 2012. Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya. Tropical Animal Health and Production 44(1): 25-31en_US
dcterms.extentp. 25-31en_US
dcterms.issued2012-09en_US
dcterms.languageenen_US
dcterms.licenseCopyrighted; all rights reserveden_US
dcterms.publisherSpringeren_US
dcterms.subjectdairiesen_US
dcterms.subjectfarming systemsen_US
dcterms.subjectlivestocken_US
dcterms.subjectzoonosesen_US
dcterms.typeJournal Articleen_US

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