Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya

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cg.contributor.affiliationUniversity of Nairobien
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.crpAgriculture for Nutrition and Health
cg.coverage.countryKenya
cg.coverage.iso3166-alpha2KE
cg.coverage.regionAfrica
cg.coverage.regionEastern Africa
cg.creator.identifierRobert A. Skilton: 0000-0003-4177-9404
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1007/s11250-012-0202-5en
cg.isijournalISI Journalen
cg.issn0049-4747en
cg.issn1573-7438en
cg.issue1en
cg.journalTropical Animal Health and Productionen
cg.reviewStatusPeer Reviewen
cg.subject.ilriAGRI-HEALTHen
cg.subject.ilriDAIRYINGen
cg.subject.ilriEMERGING DISEASESen
cg.subject.ilriFARMING SYSTEMSen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriZOONOTIC DISEASESen
cg.volume44en
dc.contributor.authorKang'ethe, Erastus K.en
dc.contributor.authorMulinge, E.K.en
dc.contributor.authorSkilton, Robert A.en
dc.contributor.authorNjahira, Moses N.en
dc.contributor.authorMonda, J.G.en
dc.contributor.authorNyongesa, C.en
dc.contributor.authorMbae, C.K.en
dc.contributor.authorKamwati, S.K.en
dc.date.accessioned2012-09-29T06:02:37Zen
dc.date.available2012-09-29T06:02:37Zen
dc.identifier.urihttps://hdl.handle.net/10568/24412
dc.titleCryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenyaen
dcterms.abstractA total of 1,734 cattle faecal samples from 296 dairy-keeping households were collected from urban settings in Nairobi, Kenya. Modified Ziehl–Neelsen staining method and an immunofluorescence assay were used to identify those samples with Cryptosporidium oocyst infection. Oocysts from positive faecal samples were isolated by Sheather's sucrose flotation method and picked from the concentrate using cover slips. Genomic DNA was extracted from 124 of the faecal samples that were positive for Cryptosporidium and was used as template for nested PCR of the 18S rRNA gene. Twenty-five samples (20 %) were PCR-positive for Cryptosporidium, and 24 of the PCR products were successfully cloned and sequenced. Sequence and phylogenetic analysis identified 17 samples (68 %) as Cryptosporidium parvum-like, four samples (16 %) as Cryptosporidium ryanae, three samples (12 %) as Cryptosporidium andersoni and one sample (4 %) as Cryptosporidium hominis. To the best of our knowledge, this is the first genotyping study to report C. parvum-like, C. andersoni and C. hominis in cattle from Kenya. The results of this study show Cryptosporidium infections in calves and cattle may be potential zoonotic reservoirs of the parasite that infects humans.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.available2012-07-15
dcterms.bibliographicCitationKang’ethe, E.K., Mulinge, E.K., Skilton, R.A., Njahira, M., Monda, J.G., Nyongesa, C., Mbae, C.K. and Kamwati, S.K. 2012. Cryptosporidium species detected in calves and cattle in Dagoretti, Nairobi, Kenya. Tropical Animal Health and Production 44(1): 25-31en
dcterms.extentp. 25-31en
dcterms.issued2012-09
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherSpringeren
dcterms.subjectdairiesen
dcterms.subjectfarming systemsen
dcterms.subjectlivestocken
dcterms.subjectzoonosesen
dcterms.typeJournal Article

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