Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation

Simple item page
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.creator.identifierVishvanath Nene: 0000-0001-7066-4169en_US
cg.identifier.doihttps://doi.org/10.1128/mbio.00204-12en_US
cg.isijournalISI Journalen_US
cg.issn2161-2129en_US
cg.issn2150-7511en_US
cg.issue5en_US
cg.journalMBioen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriLIVESTOCKen_US
cg.volume3en_US
dc.contributor.authorHayashida, K.en_US
dc.contributor.authorHara, Y.en_US
dc.contributor.authorAbe, T.en_US
dc.contributor.authorYamasaki, C.en_US
dc.contributor.authorToyoda, A.en_US
dc.contributor.authorKosuge, T.en_US
dc.contributor.authorSuzuki, Y.en_US
dc.contributor.authorSato, Y.en_US
dc.contributor.authorKawashima, S.en_US
dc.contributor.authorKatayama, T.en_US
dc.contributor.authorWakaguri, H.en_US
dc.contributor.authorInoue, N.en_US
dc.contributor.authorHomma, K.en_US
dc.contributor.authorTada-Umezaki, M.en_US
dc.contributor.authorYagi, Y.en_US
dc.contributor.authorFujii, Y.en_US
dc.contributor.authorHabara, T.en_US
dc.contributor.authorKanehisa, M.en_US
dc.contributor.authorWatanabe, H.en_US
dc.contributor.authorIto, K.en_US
dc.contributor.authorGojobori, T.en_US
dc.contributor.authorSugawara, H.en_US
dc.contributor.authorImanishi, T.en_US
dc.contributor.authorWeir, W.en_US
dc.contributor.authorGardner, M.en_US
dc.contributor.authorPain, A.en_US
dc.contributor.authorShiels, B.en_US
dc.contributor.authorHattori, M.en_US
dc.contributor.authorNene, Vishvanath M.en_US
dc.contributor.authorSugimoto, C.en_US
dc.date.accessioned2013-07-02T19:29:11Zen_US
dc.date.available2013-07-02T19:29:11Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/32849en_US
dc.titleComparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformationen_US
dcterms.abstractWe sequenced the genome of Theileria orientalis , a tick-borne apicomplexan protozoan parasite of cattle. The focus of this study was a comparative genome analysis of T . orientalis relative to other highly pathogenic Theileria species, T . parva and T . annulata . T . parva and T . annulata induce transformation of infected cells of lymphocyte or macrophage/monocyte lineages; in contrast, T . orientalis does not induce uncontrolled proliferation of infected leukocytes and multiplies predominantly within infected erythrocytes. While synteny across homologous chromosomes of the three Theileria species was found to be well conserved overall, subtelomeric structures were found to differ substantially, as T . orientalis lacks the large tandemly arrayed subtelomere-encoded variable secreted protein-encoding gene family. Moreover, expansion of particular gene families by gene duplication was found in the genomes of the two transforming Theileria species, most notably, the TashAT/TpHN and Tar/Tpr gene families. Gene families that are present only in T . parva and T . annulata and not in T . orientalis , Babesia bovis , or Plasmodium were also identified. Identification of differences between the genome sequences of Theileria species with different abilities to transform and immortalize bovine leukocytes will provide insight into proteins and mechanisms that have evolved to induce and regulate this process. The T . orientalis genome database is available at http://totdb.czc.hokudai.ac.jp/ .IMPORTANCE Cancer-like growth of leukocytes infected with malignant Theileria parasites is a unique cellular event, as it involves the transformation and immortalization of one eukaryotic cell by another. In this study, we sequenced the whole genome of a nontransforming Theileria species, Theileria orientalis , and compared it to the published sequences representative of two malignant, transforming species, T . parva and T . annulata . The genome-wide comparison of these parasite species highlights significant genetic diversity that may be associated with evolution of the mechanism(s) deployed by an intracellular eukaryotic parasite to transform its host cell.en_US
dcterms.accessRightsOpen Accessen_US
dcterms.bibliographicCitationHayashida, K., Hara, Y., Abe, T., Yamasaki, C., Toyoda, A., Kosuge, T., Suzuki, Y., Sato, Y., Kawashima, S., Katayama, T., Wakaguri, H., Inoue, N., Homma, K., Tada-Umezaki, M., Yagi, Y., Fujii, Y., Habara, T., Kanehisa, M., Watanabe, H., Ito, K., Gojobori, T., Sugawara, H., Imanishi, T., Weir, W., Gardner, M., Pain, A., Shiels, B., Hattori, M., Nene, V. and Sugimoto, C. 2012. Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation. MBio 3(5): e00204en_US
dcterms.issued2012-11en_US
dcterms.languageenen_US
dcterms.licenseCC-BY-NC-SA-3.0en_US
dcterms.publisherAmerican Society for Microbiologyen_US
dcterms.subjectparasitesen_US
dcterms.subjectlivestocken_US
dcterms.typeJournal Articleen_US

Files

License bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ILRI articles in journals
ILRI vaccine biosciences program articles