Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia

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cg.authorship.typesCGIAR and developing country instituteen_US
cg.contributor.affiliationKenya Agricultural and Livestock Research Organizationen_US
cg.contributor.affiliationVaccine Infectious Disease Organizationen_US
cg.contributor.affiliationInternational Livestock Research Instituteen_US
cg.contributor.affiliationUniversity of Nairobien_US
cg.contributor.affiliationUniversity of Bernen_US
cg.creator.identifierJan Naessens: 0000-0002-7075-9915en_US
cg.creator.identifierAnne Liljander: 0000-0002-7543-5493en_US
cg.creator.identifierJoerg Jores: 0000-0003-3790-5746en_US
cg.howPublishedFormally Publisheden_US
cg.identifier.doihttps://doi.org/10.1016/j.vetimm.2016.02.010en_US
cg.isijournalISI Journalen_US
cg.issn0165-2427en_US
cg.journalVeterinary Immunology and Immunopathologyen_US
cg.reviewStatusPeer Reviewen_US
cg.subject.ilriANIMAL DISEASESen_US
cg.subject.ilriCBPPen_US
cg.subject.ilriDISEASE CONTROLen_US
cg.subject.ilriLIVESTOCKen_US
cg.subject.ilriVACCINESen_US
cg.volume171en_US
dc.contributor.authorNkando, I.en_US
dc.contributor.authorPérez Casal, J.en_US
dc.contributor.authorMwirigi, M.en_US
dc.contributor.authorPrysliak, T.en_US
dc.contributor.authorTownsend, H.en_US
dc.contributor.authorBerberov, E.en_US
dc.contributor.authorKuria, J.en_US
dc.contributor.authorMugambi, J.en_US
dc.contributor.authorSoi, R.en_US
dc.contributor.authorLiljander, Anne M.en_US
dc.contributor.authorJores, Joergen_US
dc.contributor.authorGerdts, V.en_US
dc.contributor.authorPotter, A.en_US
dc.contributor.authorNaessens, Janen_US
dc.contributor.authorWesonga, H.O.en_US
dc.date.accessioned2016-03-28T20:40:13Zen_US
dc.date.available2016-03-28T20:40:13Zen_US
dc.identifier.urihttps://hdl.handle.net/10568/72741en_US
dc.titleRecombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumoniaen_US
dcterms.abstractMycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmmstrains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. We used a reverse vaccinology approach to identify 66 Mmm potential vaccine candidates. The selection and grouping of the antigens was based on the presence of specific antibodies in sera from CBPP-positive animals. The antigens were used to immunize male Boran cattle (Bos indicus) followed by a challenge with the Mmm strain Afadé. Two of the groups immunized with five proteins each showed protection after the Mmm challenge (Groups A and C; P < 0.05) and in one group (Group C) Mmm could not be cultured from lung specimens. A third group (Group N) showed a reduced number of animals with lesions and the cultures for Mmm were also negative. While immunization with some of the antigens conferred protection, others may have increased immune-related pathology. This is the first report that Mmm recombinant proteins have been successfully used to formulate a prototype vaccine and these results pave the way for the development of a novel commercial vaccine.en_US
dcterms.accessRightsLimited Accessen_US
dcterms.audienceScientistsen_US
dcterms.bibliographicCitationNkando, I., Perez-Casal, J., Mwirigi, M., Prysliak, T., Townsend, H., Berberov, E., Kuria, J., Mugambi, J., Soi, R., Liljander, A., Jores, J., Gerdts, V., Potter, A., Naessens, J. and Wesonga, H. 2016. Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia. Veterinary Immunology and Immunopathology 171:103–114.en_US
dcterms.extentp. 103-114en_US
dcterms.issued2016-03en_US
dcterms.languageenen_US
dcterms.licenseCopyrighted; all rights reserveden_US
dcterms.publisherElsevieren_US
dcterms.subjectanimal diseasesen_US
dcterms.subjectdisease controlen_US
dcterms.typeJournal Articleen_US

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