Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia

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cg.authorship.typesCGIAR and developing country instituteen
cg.contributor.affiliationKenya Agricultural and Livestock Research Organizationen
cg.contributor.affiliationVaccine Infectious Disease Organizationen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationUniversity of Nairobien
cg.contributor.affiliationUniversity of Bernen
cg.creator.identifierJan Naessens: 0000-0002-7075-9915
cg.creator.identifierAnne Liljander: 0000-0002-7543-5493
cg.creator.identifierJoerg Jores: 0000-0003-3790-5746
cg.howPublishedFormally Publisheden
cg.identifier.doihttps://doi.org/10.1016/j.vetimm.2016.02.010en
cg.isijournalISI Journalen
cg.issn0165-2427en
cg.journalVeterinary Immunology and Immunopathologyen
cg.reviewStatusPeer Reviewen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriCBPPen
cg.subject.ilriDISEASE CONTROLen
cg.subject.ilriLIVESTOCKen
cg.subject.ilriVACCINESen
cg.volume171en
dc.contributor.authorNkando, I.en
dc.contributor.authorPérez Casal, J.en
dc.contributor.authorMwirigi, M.en
dc.contributor.authorPrysliak, T.en
dc.contributor.authorTownsend, H.en
dc.contributor.authorBerberov, E.en
dc.contributor.authorKuria, J.en
dc.contributor.authorMugambi, J.en
dc.contributor.authorSoi, R.en
dc.contributor.authorLiljander, Anne M.en
dc.contributor.authorJores, Joergen
dc.contributor.authorGerdts, V.en
dc.contributor.authorPotter, A.en
dc.contributor.authorNaessens, Janen
dc.contributor.authorWesonga, H.O.en
dc.date.accessioned2016-03-28T20:40:13Zen
dc.date.available2016-03-28T20:40:13Zen
dc.identifier.urihttps://hdl.handle.net/10568/72741
dc.titleRecombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumoniaen
dcterms.abstractMycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP), a devastating respiratory disease mainly affecting cattle in sub-Saharan Africa. The current vaccines are based on live-attenuated Mmmstrains and present problems with temperature stability, duration of immunity and adverse reactions, thus new vaccines are needed to overcome these issues. We used a reverse vaccinology approach to identify 66 Mmm potential vaccine candidates. The selection and grouping of the antigens was based on the presence of specific antibodies in sera from CBPP-positive animals. The antigens were used to immunize male Boran cattle (Bos indicus) followed by a challenge with the Mmm strain Afadé. Two of the groups immunized with five proteins each showed protection after the Mmm challenge (Groups A and C; P < 0.05) and in one group (Group C) Mmm could not be cultured from lung specimens. A third group (Group N) showed a reduced number of animals with lesions and the cultures for Mmm were also negative. While immunization with some of the antigens conferred protection, others may have increased immune-related pathology. This is the first report that Mmm recombinant proteins have been successfully used to formulate a prototype vaccine and these results pave the way for the development of a novel commercial vaccine.en
dcterms.accessRightsLimited Access
dcterms.audienceScientistsen
dcterms.bibliographicCitationNkando, I., Perez-Casal, J., Mwirigi, M., Prysliak, T., Townsend, H., Berberov, E., Kuria, J., Mugambi, J., Soi, R., Liljander, A., Jores, J., Gerdts, V., Potter, A., Naessens, J. and Wesonga, H. 2016. Recombinant Mycoplasma mycoides proteins elicit protective immune responses against contagious bovine pleuropneumonia. Veterinary Immunology and Immunopathology 171:103–114.en
dcterms.extentp. 103-114en
dcterms.issued2016-03
dcterms.languageen
dcterms.licenseCopyrighted; all rights reserved
dcterms.publisherElsevieren
dcterms.subjectanimal diseasesen
dcterms.subjectdisease controlen
dcterms.typeJournal Article

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