Comparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strains

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cg.contributor.affiliationInstitute for Sustainable Agricultureen
cg.contributor.affiliationInternational Livestock Research Instituteen
cg.contributor.affiliationUniversity of Córdoba, Spainen
cg.contributor.donorGovernment of Cataloniaen
cg.contributor.donorMinistry of Economy, Industry and Competitivenessen
cg.creator.identifierAnna Lacasta: 0000-0001-8381-9844en
cg.identifier.doihttps://doi.org/10.1186/s13567-018-0585-zen
cg.issn1297-9716en
cg.issue1en
cg.journalVeterinary Researchen
cg.subject.ilriANIMAL DISEASESen
cg.subject.ilriASFen
cg.subject.ilriPIGSen
cg.volume49en
dc.contributor.authorHerrera-Uribe, Júberen
dc.contributor.authorJiménez-Marín, Ángelesen
dc.contributor.authorLacasta, Annaen
dc.contributor.authorMonteagudo, Paula L.en
dc.contributor.authorPina-Pedrero, Soniaen
dc.contributor.authorRodríguez, Fernandoen
dc.contributor.authorMoreno, Ángelaen
dc.contributor.authorGarrido, Juan J.en
dc.date.accessioned2023-03-10T14:40:10Zen
dc.date.available2023-03-10T14:40:10Zen
dc.identifier.urihttps://hdl.handle.net/10568/129581
dc.titleComparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strainsen
dcterms.abstractAfrican swine fever (ASF) is a pathology of pigs against which there is no treatment or vaccine. Understanding the equilibrium between innate and adaptive protective responses and immune pathology might contribute to the development of strategies against ASFV. Here we compare, using a proteomic approach, the course of the in vivo infection caused by two homologous strains: the virulent E75 and the attenuated E75CV1. Our results show a progressive loss of proteins by day 7 post-infection (pi) with E75, reflecting tissue destruction. Many signal pathways were affected by both infections but in different ways and extensions. Cytoskeletal remodelling and clathrin-endocytosis were affected by both isolates, while a greater number of proteins involved on inflammatory and immunological pathways were altered by E75CV1. 14-3-3 mediated signalling, related to immunity and apoptosis, was inhibited by both isolates. The implication of the Rho GTPases by E75CV1 throughout infection is also evident. Early events reflected the lack of E75 recognition by the immune system, an evasion strategy acquired by the virulent strains, and significant changes at 7 days post-infection (dpi), coinciding with the peak of infection and the time of death. The protein signature at day 31 pi with E75CV1 seems to reflect events observed at 1 dpi, including the upregulation of proteosomal subunits and molecules described as autoantigens (vimentin, HSPB1, enolase and lymphocyte cytosolic protein 1), which allow the speculation that auto-antibodies could contribute to chronic ASFV infections. Therefore, the use of proteomics could help understand ASFV pathogenesis and immune protection, opening new avenues for future research.en
dcterms.accessRightsOpen Accessen
dcterms.available2018-09-12en
dcterms.bibliographicCitationHerrera-Uribe, Júber; Jiménez-Marín, Ángeles; Lacasta, Anna; Monteagudo, Paula L.; Pina-Pedrero, Sonia; Rodríguez, Fernando; Moreno, Ángela; Garrido, Juan J. 2018. Comparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strains. Veterinary Research 49:en
dcterms.issued2018-12en
dcterms.languageenen
dcterms.licenseCC-BY-4.0en
dcterms.publisherSpringeren
dcterms.subjectswineen
dcterms.subjectanalysisen
dcterms.subjectvirusen
dcterms.subjectafrican swine feveren
dcterms.subjectafrican swine fever virusen
dcterms.subjectstrainsen
dcterms.subjectfeveren
dcterms.subjectnodesen
dcterms.subjectswine feveren
dcterms.subjectlymphen
dcterms.subjectswine fever virusen
dcterms.typeJournal Articleen

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